巴西亚马逊河北部树懒的人畜共患贾第虫组合A

IF 2.5 4区医学 Q2 PARASITOLOGY

Memorias do Instituto Oswaldo Cruz Pub Date : 2023-01-01 DOI:10.1590/0074-02760230088

Lisiane Lappe dos Reis, Lirna Salvioni Silva de Souza, Francisco Carlos de Oliveira Braga, Dayane Costa de Souza Lima, Natália Aparecida de Souza Lima, Jessica da Silva Padinha, Alessandra Ferreira Dales Nava, Ana Carolina Paulo Vicente

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引用次数: 0

摘要

背景十二指肠贾第鞭毛虫寄生虫感染多种脊椎动物宿主，包括家畜和野生动物以及人类。贾第鞭毛虫基因分型为8个组合(A-H)。已经在巴西亚马逊和世界各地的人类、野生动物和家畜(非人灵长类动物和猫)中发现了人畜共患病组合A和B。由于其人畜共患/人畜共患的性质，监测行动和贾第鞭毛虫聚集体的定义对于描述流行病学情况和实施进一步的控制措施非常重要。目的测定巴西亚马逊地区树懒中十二指肠棘虫的分布。方法对亚马孙州树懒粪便进行寄生虫学检查。针对β -栀子苷(BG)的聚合酶链反应(PCR)，以及多位点序列分型(MLST)方案、扩增子测序和系统发育分析的基因。在这里，我们通过显微镜鉴定了两种北方树懒(Bradypus tridactylus)的贾第虫。结果表明，这两份样本均感染了十二指肠螺旋体A组合。系统进化分析表明，它们在人类和野生及家畜的序列中属于A组合。因此，我们的研究结果除了首次表明这种寄生虫目前在树懒中存在外，还揭示了这种野生动物物种可能是巴西亚马逊地区这种寄生虫人畜共患/人畜共患情景的一部分。

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Zoonotic Giardia duodenalis assemblage A in northern sloth from Brazilian Amazon

BACKGROUND The parasite Giardia duodenalis infects a wide range of vertebrate hosts, including domestic and wild animals as well as humans. Giardia is genotyped into eight assemblages (A-H). Zoonotic assemblages A and B have already been identified in humans and wild and domestic animals (non-human primates and cats) from Brazilian Amazon and in the world. Due to its zoonotic/zooanthroponotic nature, surveillance initiatives and the definition of Giardia assemblages are important in order to characterise the epidemiological scenario and to implement further control measures. OBJECTIVES Determine assemblages of G. duodenalis in sloths from the Brazilian Amazon Region. METHODS Faecal parasitological examination of sloths from Amazonas State. Polymerase chain reaction (PCR) targeting the beta giardin (BG), and genes from multilocus sequence typing (MLST) scheme, amplicon sequencing and phylogenetic analysis. FINDINGS Here, we identified, by microscopy, Giardia in two northern sloths (Bradypus tridactylus). These two samples were submitted to molecular assays and it was revealed that both were infected by G. duodenalis assemblage A. Phylogenetic analysis showed that they belong to assemblage A within sequences from humans and wild and domestic animals. CONCLUSION Therefore, besides showing, by the first time, the current presence of this parasite in sloths, our findings reveals that this wild animal species would be part of the zoonotic/zooanthroponotic scenario of this parasite in the Brazilian Amazon.

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来源期刊

Memorias do Instituto Oswaldo Cruz 医学-寄生虫学

CiteScore

5.00

自引率

3.60%

发文量

审稿时长

3-8 weeks

期刊介绍： Memórias do Instituto Oswaldo Cruz is a journal specialized in microbes & their vectors causing human infections. This means that we accept manuscripts covering multidisciplinary approaches and findings in the basic aspects of infectious diseases, e.g. basic in research in prokariotes, eukaryotes, and/or virus. Articles must clearly show what is the main question to be answered, the hypothesis raised, and the contribution given by the study. Priority is given to manuscripts reporting novel mechanisms and general findings concerning the biology of human infectious prokariotes, eukariotes or virus. Papers reporting innovative methods for diagnostics or that advance the basic research with these infectious agents are also welcome. It is important to mention what we do not publish: veterinary infectious agents research, taxonomic analysis and re-description of species, epidemiological studies or surveys or case reports and data re-analysis. Manuscripts that fall in these cases or that are considered of low priority by the journal editorial board, will be returned to the author(s) for submission to another journal.