肽链释放因子 2a 基因通过调节线粒体功能调控玉米籽粒发育

IF 6 1区 农林科学 Q1 AGRONOMY
Hui Zhang , Yijian Feng , Kunyang Song , Guofang Li , Jiao Jin , Jingjing Gao , Yongtian Qin , Hongqiu Wang , Jinpeng Cheng , Zonghua Liu , Jihua Tang , Zhiyuan Fu
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引用次数: 0

摘要

线粒体蛋白质翻译对有氧能量生产至关重要,包括线粒体核糖体循环的四个基本步骤,即启动、延伸、多肽终止和核糖体循环。当终止密码子进入线粒体核糖体的 A 位点时,翻译终止就开始了。然而,参与植物线粒体(尤其是单子叶植物)翻译的 RF 和机制在很大程度上仍不为人知。在这里,我们发现了一种皱缩核仁(crk5 等位基因)突变体,其核仁大小、百粒重显著降低,并具有胚胎致死的表型。通过基于图谱的克隆分离出了 Crk5 等位基因,发现它编码线粒体定位 RF2a。由于它是拟南芥线粒体 RF2a 的直向同源物,我们将该基因命名为 ZmmtRF2a。ZmmtRF2a缺失了crk5的第5-7个外显子,导致含有GGQ和SPF的结构域缺失,而这两个结构域对于RF的释放活性、线粒体核糖体结合和终止密码子识别至关重要。Western 印迹和 qRT-PCR 分析表明,crk5 突变导致线粒体结构和功能异常。耐人寻味的是,我们观察到了 crk5 的反馈回路,检测到一些线粒体核糖体和线粒体相关成分的转录水平上调,特别是线粒体复合物 CI、CIV 和与核糖体组装相关的 PPR。总之,我们的数据支持 ZmmtRF2a 在调控玉米线粒体结构和功能中的关键作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A peptide chain release factor 2a gene regulates maize kernel development by modulating mitochondrial function

Mitochondrial protein translation that is essential for aerobic energy production includes four essential steps of the mitochondrial ribosome cycle, namely, initiation, elongation, termination of the polypeptide, and ribosome recycling. Translation termination initiates when a stop codon enters the A site of the mitochondrial ribosome where it is recognized by a dedicated peptide release factor (RF). However, RFs and mechanisms involved in translation in plant mitochondria, especially in monocotyledons, remain largely unknown. Here, we identified a crumpled kernel (crk5 allele) mutant, with significantly decreased kernel size, 100-kernel weight, and an embryo-lethal phenotype. The Crk5 allele was isolated using map-based cloning and found to encode a mitochondrial localization RF2a. As it is an ortholog of Arabidopsis mitochondrial RF2a, we named the gene ZmmtRF2a. ZmmtRF2a is missing the 5th–7th exons in the crk5 resulting in deletion of domains containing motifs GGQ and SPF that are essential for release activity of RF, mitochondrial ribosome binding, and stop codon recognition. Western blot and qRT-PCR analyses indicate that the crk5 mutation results in abnormal mitochondrion structure and function. Intriguingly, we observed a feedback loop in the crk5 with up-regulated transcript levels detected for several mitochondrial ribosome and mitochondrial-related components, in particular mitochondrial complexes CI, CIV, and a ribosome assembly related PPR. Together, our data support a crucial role for ZmmtRF2a in regulation of mitochondrial structure and function in maize.

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来源期刊
Crop Journal
Crop Journal Agricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
9.90
自引率
3.00%
发文量
638
审稿时长
41 days
期刊介绍: The major aims of The Crop Journal are to report recent progresses in crop sciences including crop genetics, breeding, agronomy, crop physiology, germplasm resources, grain chemistry, grain storage and processing, crop management practices, crop biotechnology, and biomathematics. The regular columns of the journal are Original Research Articles, Reviews, and Research Notes. The strict peer-review procedure will guarantee the academic level and raise the reputation of the journal. The readership of the journal is for crop science researchers, students of agricultural colleges and universities, and persons with similar academic levels.
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