Hui Zhang , Yijian Feng , Kunyang Song , Guofang Li , Jiao Jin , Jingjing Gao , Yongtian Qin , Hongqiu Wang , Jinpeng Cheng , Zonghua Liu , Jihua Tang , Zhiyuan Fu
{"title":"肽链释放因子 2a 基因通过调节线粒体功能调控玉米籽粒发育","authors":"Hui Zhang , Yijian Feng , Kunyang Song , Guofang Li , Jiao Jin , Jingjing Gao , Yongtian Qin , Hongqiu Wang , Jinpeng Cheng , Zonghua Liu , Jihua Tang , Zhiyuan Fu","doi":"10.1016/j.cj.2023.10.006","DOIUrl":null,"url":null,"abstract":"<div><p>Mitochondrial protein translation that is essential for aerobic energy production includes four essential steps of the mitochondrial ribosome cycle, namely, initiation, elongation, termination of the polypeptide, and ribosome recycling. Translation termination initiates when a stop codon enters the A site of the mitochondrial ribosome where it is recognized by a dedicated peptide release factor (RF). However, RFs and mechanisms involved in translation in plant mitochondria, especially in monocotyledons, remain largely unknown. Here, we identified a <em>crumpled kernel</em> (<em>crk5</em> allele) mutant, with significantly decreased kernel size, 100-kernel weight, and an embryo-lethal phenotype. The <em>Crk5</em> allele was isolated using map-based cloning and found to encode a mitochondrial localization RF2a. As it is an ortholog of Arabidopsis mitochondrial RF2a, we named the gene <em>ZmmtRF2a</em>. <em>ZmmtRF2a</em> is missing the 5th–7th exons in the <em>crk5</em> resulting in deletion of domains containing motifs GGQ and SPF that are essential for release activity of RF, mitochondrial ribosome binding, and stop codon recognition. Western blot and qRT-PCR analyses indicate that the <em>crk5</em> mutation results in abnormal mitochondrion structure and function. Intriguingly, we observed a feedback loop in the <em>crk5</em> with up-regulated transcript levels detected for several mitochondrial ribosome and mitochondrial-related components, in particular mitochondrial complexes CI, CIV, and a ribosome assembly related PPR. Together, our data support a crucial role for <em>ZmmtRF2a</em> in regulation of mitochondrial structure and function in maize.</p></div>","PeriodicalId":10790,"journal":{"name":"Crop Journal","volume":null,"pages":null},"PeriodicalIF":6.0000,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2214514123001447/pdfft?md5=cd2a796956f83745002de05ccb91715f&pid=1-s2.0-S2214514123001447-main.pdf","citationCount":"0","resultStr":"{\"title\":\"A peptide chain release factor 2a gene regulates maize kernel development by modulating mitochondrial function\",\"authors\":\"Hui Zhang , Yijian Feng , Kunyang Song , Guofang Li , Jiao Jin , Jingjing Gao , Yongtian Qin , Hongqiu Wang , Jinpeng Cheng , Zonghua Liu , Jihua Tang , Zhiyuan Fu\",\"doi\":\"10.1016/j.cj.2023.10.006\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Mitochondrial protein translation that is essential for aerobic energy production includes four essential steps of the mitochondrial ribosome cycle, namely, initiation, elongation, termination of the polypeptide, and ribosome recycling. Translation termination initiates when a stop codon enters the A site of the mitochondrial ribosome where it is recognized by a dedicated peptide release factor (RF). However, RFs and mechanisms involved in translation in plant mitochondria, especially in monocotyledons, remain largely unknown. Here, we identified a <em>crumpled kernel</em> (<em>crk5</em> allele) mutant, with significantly decreased kernel size, 100-kernel weight, and an embryo-lethal phenotype. The <em>Crk5</em> allele was isolated using map-based cloning and found to encode a mitochondrial localization RF2a. As it is an ortholog of Arabidopsis mitochondrial RF2a, we named the gene <em>ZmmtRF2a</em>. <em>ZmmtRF2a</em> is missing the 5th–7th exons in the <em>crk5</em> resulting in deletion of domains containing motifs GGQ and SPF that are essential for release activity of RF, mitochondrial ribosome binding, and stop codon recognition. Western blot and qRT-PCR analyses indicate that the <em>crk5</em> mutation results in abnormal mitochondrion structure and function. Intriguingly, we observed a feedback loop in the <em>crk5</em> with up-regulated transcript levels detected for several mitochondrial ribosome and mitochondrial-related components, in particular mitochondrial complexes CI, CIV, and a ribosome assembly related PPR. Together, our data support a crucial role for <em>ZmmtRF2a</em> in regulation of mitochondrial structure and function in maize.</p></div>\",\"PeriodicalId\":10790,\"journal\":{\"name\":\"Crop Journal\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":6.0000,\"publicationDate\":\"2023-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2214514123001447/pdfft?md5=cd2a796956f83745002de05ccb91715f&pid=1-s2.0-S2214514123001447-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Crop Journal\",\"FirstCategoryId\":\"91\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2214514123001447\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Crop Journal","FirstCategoryId":"91","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2214514123001447","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}
A peptide chain release factor 2a gene regulates maize kernel development by modulating mitochondrial function
Mitochondrial protein translation that is essential for aerobic energy production includes four essential steps of the mitochondrial ribosome cycle, namely, initiation, elongation, termination of the polypeptide, and ribosome recycling. Translation termination initiates when a stop codon enters the A site of the mitochondrial ribosome where it is recognized by a dedicated peptide release factor (RF). However, RFs and mechanisms involved in translation in plant mitochondria, especially in monocotyledons, remain largely unknown. Here, we identified a crumpled kernel (crk5 allele) mutant, with significantly decreased kernel size, 100-kernel weight, and an embryo-lethal phenotype. The Crk5 allele was isolated using map-based cloning and found to encode a mitochondrial localization RF2a. As it is an ortholog of Arabidopsis mitochondrial RF2a, we named the gene ZmmtRF2a. ZmmtRF2a is missing the 5th–7th exons in the crk5 resulting in deletion of domains containing motifs GGQ and SPF that are essential for release activity of RF, mitochondrial ribosome binding, and stop codon recognition. Western blot and qRT-PCR analyses indicate that the crk5 mutation results in abnormal mitochondrion structure and function. Intriguingly, we observed a feedback loop in the crk5 with up-regulated transcript levels detected for several mitochondrial ribosome and mitochondrial-related components, in particular mitochondrial complexes CI, CIV, and a ribosome assembly related PPR. Together, our data support a crucial role for ZmmtRF2a in regulation of mitochondrial structure and function in maize.
Crop JournalAgricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
9.90
自引率
3.00%
发文量
638
审稿时长
41 days
期刊介绍:
The major aims of The Crop Journal are to report recent progresses in crop sciences including crop genetics, breeding, agronomy, crop physiology, germplasm resources, grain chemistry, grain storage and processing, crop management practices, crop biotechnology, and biomathematics.
The regular columns of the journal are Original Research Articles, Reviews, and Research Notes. The strict peer-review procedure will guarantee the academic level and raise the reputation of the journal. The readership of the journal is for crop science researchers, students of agricultural colleges and universities, and persons with similar academic levels.