同时使用新一代测序分析的Nested Multiplex PCR法，对赖氏菌的耐药性和型别进行新的同时鉴定法

Q4 Medicine

Japanese Journal of Leprosy Pub Date : 2023-01-01 DOI:10.5025/hansen.92.47

Yasuhisa Iwao, Shuichi Mori, Manabu Ato, Noboru Nakata

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引用次数: 0

摘要

麻风分枝杆菌是麻风的主要病原，其基因型可分为4个单核苷酸多态性(SNP)型和16个亚型。麻风分枝杆菌的耐药性和基因型通常是用PCR和桑格DNA测序来确定的，但是这需要付出很大的努力。本文介绍了一种将巢式多重PCR与下一代序列分析相结合，直接从临床标本中快速鉴定麻风分枝杆菌耐药性和SNP基因型的方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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次世代シーケンシング解析を併用したNested Multiplex PCR法による、らい菌の薬剤耐性および型別の新規同時同定法

Mycobacterium leprae is the major etiologic agent of leprosy, and its genotype can be divided into four single nucleotide polymorphism (SNP) types and 16 subtypes. Drug resistance and genotype of M. leprae are typically determined using PCR and Sanger DNA sequencing, however, this requires a great deal of effort. In this review, we present a rapid method that we developed to identify drug resistance and SNP genotype of M. leprae directly from clinical specimens by combining nested multiplex PCR with next generation sequence analysis.

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来源期刊

Japanese Journal of Leprosy Medicine-Dermatology

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