枯草芽孢杆菌菌株特性对小麦接种培养发育的影响

Anastasia Honchar, Oksana Tonkha, Mykola Patyka
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引用次数: 0

摘要

研究枯草芽孢杆菌菌株对植物生长发育有积极影响,并在最佳细菌负荷下表现出刺激作用,这就预先确定了研究的相关性。该研究对农业、生态学和可持续发展具有重要意义。研究了枯草芽孢杆菌新菌株(H3、H10、H13、H36、H38、H40、H43、H45)与接种作物接种后对冬小麦(Triticum aestivum L.)幼苗发育的影响。研究采用实验室、植被、数理统计等方法:菌株深耕培养,试验植物种子滚发芽法,数据处理采用Statistica 8.0和MS Excel。综述了不同工艺形式和不同稀释度下枯草芽孢杆菌菌株培养液对小麦试验植株生长发育影响的模型试验结果。已经确定,在1:10、1:50、1:100和1:500的稀释度下,可以观察到生物制剂的刺激作用,并在1:100的稀释度下达到最大效果。研究表明,枯草芽孢杆菌接种剂对成熟技术形式的种子(孢子培养,每粒种子2.0 × 107个细胞)萌发的积极影响最大。与对照相比,与枯草芽孢杆菌接种的小麦种子萌发能提高了96.5%,幼苗生重提高了84.0 ~ 109.6%,表明新品系具有促生长的特性。结果表明,使用枯草芽孢杆菌H38、H40和H45的成熟培养物可使根重比未杀菌的对照增加4.8 ~ 11.3%。当枯草芽孢杆菌H3、H10、H13、H36、H43培养液以营养细胞形式处理时,根质量比对照减少11.8% ~ 44.0%。所研究的枯草芽孢杆菌对冬小麦的生长发育有明显的影响,是一种很有前景的促生剂。本研究的实际意义在于了解和确定枯草芽孢杆菌菌株促进植物特别是冬小麦生长发育的潜在益处。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Peculiarities of Bacillus Subtilis strains influence on the development of Triticum Aestivum L. in inoculative cultures
The research relevance is predetermined by the need to study strains of Bacillus subtilis bacteria that have a positive effect on plant growth and development and exhibit a stimulating effect at optimal bacterial loads. The research is of great importance for agriculture, ecology, and sustainable development. The research aims to determine the effect of new strains of B. subtilis (H3, H10, H13, H36, H38, H40, H43, H45) on the development of winter wheat (Triticum aestivum L.) seedlings when inoculated with inoculated crops. Laboratory, vegetation, and mathematical and statistical methods were used in the study: deep cultivation of strains, roll method of germination of test plant seeds, and data processing using Statistica 8.0 and MS Excel. The results of the model experiment on the effect of culture liquids of B. subtilis strains under different technological forms and dilutions on the growth and development of wheat test plants are summarised. It has been established that at dilutions of 1:10, 1:50, 1:100, and 1:500, the stimulating effect of bioagents is observed, and the maximum effect is achieved at a dilution of 1:100. It has been shown that the greatest positive effect on wheat seed germination was observed for B. subtilis inoculants applied to seeds in mature technological forms (spore culture, 2.0 x 107 cells per seed). The germination energy of Triticum aestivum L. seeds increased by 96.5% when interacting with B. subtilis inoculants, and the raw weight of seedlings increased by 84.0-109.6% depending on the experiment variant compared to the control, which indicates the growth-stimulating properties of the new strains. It was proved that the use of mature cultures of B. subtilis H38, H40 and H45 resulted in an increase in root weight by 4.8-11.3% compared to the control without bacterisation. When treated with culture fluids of B. subtilis H3, H10, H13, H36, and H43 in the form of vegetative cells, the root mass decreased by 11.8-44.0% compared to the control. The use of the studied strains of B. subtilis effectively affects the development of winter wheat and is a promising inoculant with a growth-stimulating effect. The practical significance of the study is to understand and determine the potential benefits of using Bacillus subtilis bacterial strains to enhance the growth and development of plants, in particular winter wheat (Triticum aestivum L.)
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