Wangping Zhang , Kaikai Dong , Jing Sun , Zhiwei Huang , Ping Shi
{"title":"小鼠死后时间与蛋白质变化的关系","authors":"Wangping Zhang , Kaikai Dong , Jing Sun , Zhiwei Huang , Ping Shi","doi":"10.1016/j.jflm.2023.102618","DOIUrl":null,"url":null,"abstract":"<div><p><span><span>Postmortem interval (PMI) estimation is important for forensic pathological autopsy. It has been reported that there is a correlation between certain protein changes in cadavers and PMI. However, no specific protein(s) has been used to determine the PMI so far. In this study, the total protein contents of mouse liver and spleen at different time of death were measured. The data showed that they were negatively correlated with the PMI. The degradation of β-actin was found to be positively correlated with the PMI in the liver. Additionally, proteomic technique was used to study the changes of </span>protein expression related to PMI in the liver of mice. By using Two-dimensional </span>electrophoresis<span><span>, the expressions of four proteins were found to be significantly decreased and those of other three proteins were unchanged with the increase of PMI. Among the seven proteins, six were identified with peptide mass fingerprinting<span><span> using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The three altered proteins were SBP2, </span>ENOA, </span></span>ALDH2<span> and three unchanged ones were 3HAO<span><span>, TPIS, </span>CATA, respectively. In the future, those unchanged proteins could be used as internal references to more accurately and reliably infer the time of death by assessing the level of changed proteins.</span></span></span></p></div>","PeriodicalId":16098,"journal":{"name":"Journal of forensic and legal medicine","volume":null,"pages":null},"PeriodicalIF":1.2000,"publicationDate":"2023-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The relationship between postmortem interval and protein changes in mice\",\"authors\":\"Wangping Zhang , Kaikai Dong , Jing Sun , Zhiwei Huang , Ping Shi\",\"doi\":\"10.1016/j.jflm.2023.102618\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><span><span>Postmortem interval (PMI) estimation is important for forensic pathological autopsy. It has been reported that there is a correlation between certain protein changes in cadavers and PMI. However, no specific protein(s) has been used to determine the PMI so far. In this study, the total protein contents of mouse liver and spleen at different time of death were measured. The data showed that they were negatively correlated with the PMI. The degradation of β-actin was found to be positively correlated with the PMI in the liver. Additionally, proteomic technique was used to study the changes of </span>protein expression related to PMI in the liver of mice. By using Two-dimensional </span>electrophoresis<span><span>, the expressions of four proteins were found to be significantly decreased and those of other three proteins were unchanged with the increase of PMI. Among the seven proteins, six were identified with peptide mass fingerprinting<span><span> using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The three altered proteins were SBP2, </span>ENOA, </span></span>ALDH2<span> and three unchanged ones were 3HAO<span><span>, TPIS, </span>CATA, respectively. In the future, those unchanged proteins could be used as internal references to more accurately and reliably infer the time of death by assessing the level of changed proteins.</span></span></span></p></div>\",\"PeriodicalId\":16098,\"journal\":{\"name\":\"Journal of forensic and legal medicine\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.2000,\"publicationDate\":\"2023-11-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of forensic and legal medicine\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1752928X23001361\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"MEDICINE, LEGAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of forensic and legal medicine","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1752928X23001361","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MEDICINE, LEGAL","Score":null,"Total":0}
The relationship between postmortem interval and protein changes in mice
Postmortem interval (PMI) estimation is important for forensic pathological autopsy. It has been reported that there is a correlation between certain protein changes in cadavers and PMI. However, no specific protein(s) has been used to determine the PMI so far. In this study, the total protein contents of mouse liver and spleen at different time of death were measured. The data showed that they were negatively correlated with the PMI. The degradation of β-actin was found to be positively correlated with the PMI in the liver. Additionally, proteomic technique was used to study the changes of protein expression related to PMI in the liver of mice. By using Two-dimensional electrophoresis, the expressions of four proteins were found to be significantly decreased and those of other three proteins were unchanged with the increase of PMI. Among the seven proteins, six were identified with peptide mass fingerprinting using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The three altered proteins were SBP2, ENOA, ALDH2 and three unchanged ones were 3HAO, TPIS, CATA, respectively. In the future, those unchanged proteins could be used as internal references to more accurately and reliably infer the time of death by assessing the level of changed proteins.
期刊介绍:
The Journal of Forensic and Legal Medicine publishes topical articles on aspects of forensic and legal medicine. Specifically the Journal supports research that explores the medical principles of care and forensic assessment of individuals, whether adult or child, in contact with the judicial system. It is a fully peer-review hybrid journal with a broad international perspective.
The Journal accepts submissions of original research, review articles, and pertinent case studies, editorials, and commentaries in relevant areas of Forensic and Legal Medicine, Context of Practice, and Education and Training.
The Journal adheres to strict publication ethical guidelines, and actively supports a culture of inclusive and representative publication.