苍术内酯通过ERK途径促进复发性自然流产中滋养细胞的增殖和迁移

IF 0.6 4区医学 Q4 PHARMACOLOGY & PHARMACY

Tropical Journal of Pharmaceutical Research Pub Date : 2023-09-15 DOI:10.4314/tjpr.v22i8.3

Beili Lv, Haiyan Wang, Xinrong Li

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引用次数: 0

摘要

目的:探讨白术内酯治疗复发性自然流产(RSA)的疗效。方法:建立HTR-8/SVneo体外RSA细胞模型。采用CCK8和BrdU染色法检测细胞活力和增殖，采用细胞划痕法和transwell法检测细胞迁移和侵袭。结果:苍术内酯能显著提高细胞活力，增加HTR-8/SVneo brdu阳性细胞数量(p <0.01)。苍术内酯还能显著促进细胞迁移和侵袭(p <MMP-9、MMP-2和N-cadherin蛋白表达升高，Ecadherin蛋白表达降低。苍术内酯也增加了ERK的磷酸化(p <0.01)。结论:苍术内酯通过激活ERK信号通路促进HTR-8/SVneo细胞增殖和迁移。建议使用动物模型进行进一步的研究，以确定白术内酯对体内RSA的保护作用。

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Atractylenolide promotes trophoblast cell proliferation and migration in recurrent spontaneous abortion via ERK pathway

Purpose: To investigate the effect of atractylenolide on recurrent spontaneous abortion (RSA).Methods: The HTR-8/SVneo was established as an in vitro cell model of RSA. Cell viability and proliferation were determined using CCK8 and BrdU staining, while cell migration and invasion were determined by cell scratch and transwell assays.Results: Atractylenolide significantly increased cell viability, and enhanced the number of BrdU-positive cells of HTR-8/SVneo (p < 0.01). Atractylenolide also significantly promoted cell migration and invasion (p < 0.01), and increased protein expression of MMP-9, MMP-2, and N-cadherin, but reduced Ecadherin. Atractylenolide also increased the phosphorylation of ERK (p < 0.01).Conclusion: Atractylenolide enhances cell proliferation and migration of HTR-8/SVneo through activation of ERK signaling. Further studies using animal models are recommended to determine the protective role of atractylenolide against RSA, in vivo.

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来源期刊

Tropical Journal of Pharmaceutical Research 医学-药学

CiteScore

1.00

自引率

33.30%

发文量

490

审稿时长

4-8 weeks

期刊介绍： We seek to encourage pharmaceutical and allied research of tropical and international relevance and to foster multidisciplinary research and collaboration among scientists, the pharmaceutical industry and the healthcare professionals. We publish articles in pharmaceutical sciences and related disciplines (including biotechnology, cell and molecular biology, drug utilization including adverse drug events, medical and other life sciences, and related engineering fields). Although primarily devoted to original research papers, we welcome reviews on current topics of special interest and relevance.