二倍体野生马铃薯Solanum bulbocastanum中Rpi-blb4的鉴定和绘图

IF 6 1区 农林科学 Q1 AGRONOMY
Jie Li , Amanpreet Kaur , Brian Harrower , Miles Armstrong , Daolong Dou , Xiaodan Wang , Ingo Hein
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引用次数: 0

摘要

在造成爱尔兰马铃薯饥荒170多年后,晚疫病仍被认为是最具破坏性的作物病害之一。商业马铃薯育种工作依赖于天然抗性来源,以保护作物免受快速发展的晚疫病病原体Phytophthora infestans的侵害。我们从野生二倍体马铃薯物种Solanum bulbocastanum中鉴定并绘制了一个新型广谱抗病基因,该基因对P. infestans有效。诊断性抗性基因富集测序(dRenSeq)被用来确认所鉴定的抗性的独特性。基于 RenSeq 和 GenSeq 的抗性(称为 Rpi-blb4)图谱以及重组筛选,将抗性基因座定位在马铃薯第 5 号染色体上。该区间跨度约为 2.3 Mb,与 DM 参考基因组的 11.25 和 13.56 Mb 位置相对应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Identification and mapping of Rpi-blb4 in diploid wild potato species Solanum bulbocastanum

More than 170 years after causing the potato famine in Ireland, late blight is still considered one of the most devastating crop diseases. Commercial potato breeding efforts depend on natural sources of resistance to protect crops from the rapidly evolving late blight pathogen, Phytophthora infestans. We have identified and mapped a novel broad-spectrum disease resistance gene effective against P. infestans from the wild, diploid potato species Solanum bulbocastanum. Diagnostic resistance gene enrichment sequencing (dRenSeq) was used to confirm the uniqueness of the identified resistance. RenSeq and GenSeq-based mapping of the resistance, referred to as Rpi-blb4, alongside recombinant screening, positioned the locus responsible for the resistance to potato chromosome 5. The interval spans approximately 2.3 Mb and corresponds to the DM reference genome positions of 11.25 and 13.56 Mb.

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来源期刊
Crop Journal
Crop Journal Agricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
9.90
自引率
3.00%
发文量
638
审稿时长
41 days
期刊介绍: The major aims of The Crop Journal are to report recent progresses in crop sciences including crop genetics, breeding, agronomy, crop physiology, germplasm resources, grain chemistry, grain storage and processing, crop management practices, crop biotechnology, and biomathematics. The regular columns of the journal are Original Research Articles, Reviews, and Research Notes. The strict peer-review procedure will guarantee the academic level and raise the reputation of the journal. The readership of the journal is for crop science researchers, students of agricultural colleges and universities, and persons with similar academic levels.
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