Jie Li , Amanpreet Kaur , Brian Harrower , Miles Armstrong , Daolong Dou , Xiaodan Wang , Ingo Hein
{"title":"二倍体野生马铃薯Solanum bulbocastanum中Rpi-blb4的鉴定和绘图","authors":"Jie Li , Amanpreet Kaur , Brian Harrower , Miles Armstrong , Daolong Dou , Xiaodan Wang , Ingo Hein","doi":"10.1016/j.cj.2023.08.005","DOIUrl":null,"url":null,"abstract":"<div><p>More than 170 years after causing the potato famine in Ireland, late blight is still considered one of the most devastating crop diseases. Commercial potato breeding efforts depend on natural sources of resistance to protect crops from the rapidly evolving late blight pathogen, <em>Phytophthora infestans.</em> We have identified and mapped a novel broad-spectrum disease resistance gene effective against <em>P. infestans</em> from the wild, diploid potato species <em>Solanum bulbocastanum</em>. Diagnostic resistance gene enrichment sequencing (dRenSeq) was used to confirm the uniqueness of the identified resistance. RenSeq and GenSeq-based mapping of the resistance, referred to as <em>Rpi-blb4</em>, alongside recombinant screening, positioned the locus responsible for the resistance to potato chromosome 5. The interval spans approximately 2.3 Mb and corresponds to the DM reference genome positions of 11.25 and 13.56 Mb.</p></div>","PeriodicalId":10790,"journal":{"name":"Crop Journal","volume":"11 6","pages":"Pages 1828-1835"},"PeriodicalIF":6.0000,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2214514123001150/pdfft?md5=9fc84631d97e019388ea25976639d4c1&pid=1-s2.0-S2214514123001150-main.pdf","citationCount":"0","resultStr":"{\"title\":\"Identification and mapping of Rpi-blb4 in diploid wild potato species Solanum bulbocastanum\",\"authors\":\"Jie Li , Amanpreet Kaur , Brian Harrower , Miles Armstrong , Daolong Dou , Xiaodan Wang , Ingo Hein\",\"doi\":\"10.1016/j.cj.2023.08.005\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>More than 170 years after causing the potato famine in Ireland, late blight is still considered one of the most devastating crop diseases. Commercial potato breeding efforts depend on natural sources of resistance to protect crops from the rapidly evolving late blight pathogen, <em>Phytophthora infestans.</em> We have identified and mapped a novel broad-spectrum disease resistance gene effective against <em>P. infestans</em> from the wild, diploid potato species <em>Solanum bulbocastanum</em>. Diagnostic resistance gene enrichment sequencing (dRenSeq) was used to confirm the uniqueness of the identified resistance. RenSeq and GenSeq-based mapping of the resistance, referred to as <em>Rpi-blb4</em>, alongside recombinant screening, positioned the locus responsible for the resistance to potato chromosome 5. The interval spans approximately 2.3 Mb and corresponds to the DM reference genome positions of 11.25 and 13.56 Mb.</p></div>\",\"PeriodicalId\":10790,\"journal\":{\"name\":\"Crop Journal\",\"volume\":\"11 6\",\"pages\":\"Pages 1828-1835\"},\"PeriodicalIF\":6.0000,\"publicationDate\":\"2023-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2214514123001150/pdfft?md5=9fc84631d97e019388ea25976639d4c1&pid=1-s2.0-S2214514123001150-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Crop Journal\",\"FirstCategoryId\":\"91\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2214514123001150\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Crop Journal","FirstCategoryId":"91","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2214514123001150","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}
Identification and mapping of Rpi-blb4 in diploid wild potato species Solanum bulbocastanum
More than 170 years after causing the potato famine in Ireland, late blight is still considered one of the most devastating crop diseases. Commercial potato breeding efforts depend on natural sources of resistance to protect crops from the rapidly evolving late blight pathogen, Phytophthora infestans. We have identified and mapped a novel broad-spectrum disease resistance gene effective against P. infestans from the wild, diploid potato species Solanum bulbocastanum. Diagnostic resistance gene enrichment sequencing (dRenSeq) was used to confirm the uniqueness of the identified resistance. RenSeq and GenSeq-based mapping of the resistance, referred to as Rpi-blb4, alongside recombinant screening, positioned the locus responsible for the resistance to potato chromosome 5. The interval spans approximately 2.3 Mb and corresponds to the DM reference genome positions of 11.25 and 13.56 Mb.
Crop JournalAgricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
9.90
自引率
3.00%
发文量
638
审稿时长
41 days
期刊介绍:
The major aims of The Crop Journal are to report recent progresses in crop sciences including crop genetics, breeding, agronomy, crop physiology, germplasm resources, grain chemistry, grain storage and processing, crop management practices, crop biotechnology, and biomathematics.
The regular columns of the journal are Original Research Articles, Reviews, and Research Notes. The strict peer-review procedure will guarantee the academic level and raise the reputation of the journal. The readership of the journal is for crop science researchers, students of agricultural colleges and universities, and persons with similar academic levels.