链霉菌液体培养物对埃及伊蚊幼虫的杀幼虫活性

Elkawnie: journal of islamic science and technology Pub Date : 2023-01-18 DOI:10.22373/ekw.v8i2.11436

Ni Wayan Desi Bintari, Putu Ayu Parwati

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引用次数: 0

摘要

摘要:未来，热带地区在控制登革热出血热媒介方面将面临重大挑战。对化学防治的抗性促使了以生物防治为基础的防治策略的发展。本研究旨在检测链霉菌液体培养物对埃及伊蚊幼虫的杀虫活性。通过几丁质酶试验选择链霉菌作为生物杀幼虫剂。在生物质培养基中培养几丁质酶产菌。本研究采用完全随机设计。本研究结果可从泥质土壤中分离出4株链霉菌。其中Streptomyces sp.4在体外试验中表现出几丁质溶解活性;因此，它被用于杀幼虫。Duncan试验结果显示，与阴性对照相比，Streptomyces sp.4培养物对幼虫死亡率有显著影响(p < 0.05)。A6B1处理的幼虫死亡率最高，为34.35%。Probit检验结果显示，Streptomyces sp.4培养物的LD50值为24.6±5.4 mL。体内试验表明，Streptomyces sp.4液体培养物对埃及伊蚊幼虫的死亡率有影响，与阴性对照有显著差异(p<0.05)。Streptomyces sp.4被认为是一种潜在的生物杀幼虫剂。摘要/ abstract摘要:登革热(DBD)的病媒媒介为热带大鼠(Daerah tropis memiliki tantangan besar kedepannya dalam pengendalian)。Adanya kejadian resistensi pengendalian dengan zat kimiawi mendoong strategi pengenbangan基础pengendalian生物学。企鹅，繁殖，链霉菌，埃及沙蚊，埃及沙蚊。seleksistreptomomyces sebagai biolarvasida dilakukan dengan ukikiactivititas kitinase。企鹅在体内的生物多样性。然干干，梦古那干，然干干，阿卡，冷卡。吉隆坡链霉菌的分离。Diantara keempat分离株，yyitu Streptomyces sp.4 menunjukkan aktivitas kitinolitik sehinga digunakan untuk uji aktivitas larvasida secara体内分离株。哈萨克哈萨克哈萨克哈萨克哈萨克哈萨克哈萨克哈萨克哈萨克哈萨克哈萨克哈萨克哈萨克哈萨克哈萨克哈萨克哈萨克哈萨克哈萨克哈萨克哈萨克哈萨克哈萨克哈萨克哈萨克哈萨克哈萨克哈萨克。研究结果表明:(1)研究结果表明:(1)研究结果表明:(1)研究结果表明:(1)研究结果表明:(1)研究结果表明:Hasil uji Probit menunjukkan nilai LD50达培养物Streptomyces sp.4 adalah 24.6±5.4 mL。Berdasarkan Hasil uji体内diketahui bahwa perlakuan培养物cair Streptomyces sp.4 berpengaruh terhadap tingkat kematian幼虫埃及伊蚊和berbeda nyata dengan对照阴性(p<0.05)。链霉菌sp. 4 diketahui memoriliki potentii sebagai salah satagen larvasibiology。

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Larvicidal Activity of Streptomyces sp. Liquid Cultures Against Aedes aegypti Larvae

Abstract: The tropics have significant future challenges in controlling the vectors of dengue hemorrhagic fever. The occurrence of resistance to chemical control encourages the development of strategies based on biological control. This study aimed to test the larvicidal activity of Streptomyces sp. liquid culture toward A. aegypti larvae. The selection of Streptomyces as a bio-larvicide was carried out by a chitinase test. Chitinase-producing bacteria were cultivated in biomass culture. The study was conducted using a completely randomized design. The results of this study can be isolated 4 Streptomyces isolates from muddy soil. Among the isolates, Streptomyces sp.4 showed chitinolytic activity on in vitro tests; therefore, it was used for larvicidal activity. Based on the Duncan test result, Streptomyces sp.4 culture showed a significant effect on larval mortality compared to the negative control (p<0.05). The highest rate of larval mortality was found in the A6B1 treatment (34.35%). The results of the Probit test showed that the LD50 value of the Streptomyces sp.4 culture was 24.6±5.4 mL. Based on the in vivo test, showed that Streptomyces sp.4 liquid culture affected the mortality rate of A. aegypti larvae and was significantly different from the negative control (p<0.05). Streptomyces sp.4 is known to have potential benefits as a biological larvicidal agent.Abstrak: Daerah tropis memiliki tantangan besar kedepannya dalam pengendalian vektor demam berdarah dengue (DBD). Adanya kejadian resistensi pengendalian dengan zat kimiawi mendorong strategi pengembangan berbasis pengendalian biologis. Penelitian ini bertujuan untuk melakukan pengujian aktivitas kultur Streptomyces sp. sebagai larvasida A. aegypti. Seleksi Streptomyces sebagai biolarvasida dilakukan dengan uji aktivitas kitinase. Bakteri penghasil kitinase dilakukan kultivasi kultur biomassa untuk pengujian in vivo. Rancangan uji menggunakan Rancangan Acak Lengkap. Hasil penelitian dapat diisolasi 4 isolat Streptomyces yang diisolasi dari tanah berlumpur. Diantara keempat isolat, satu isolat yaitu Streptomyces sp.4 menunjukkan aktivitas kitinolitik sehingga digunakan untuk uji aktivitas larvasida secara in vivo. Hasil uji larvasida menunjukkan perlakuan kultur Streptomyces sp.4 berpengaruh nyata terhadap persentase kematian larva dibandingkan kontrol negatif (p<0.05) berdasarkan hasil uji Duncan. Persentase kematian tertinggi didapatkan pada perlakuan A6B1 yaitu sebesar 34.35%. Hasil uji Probit menunjukkan nilai LD50 dari kultur Streptomyces sp.4 adalah 24.6 ± 5.4 mL. Berdasarkan hasil uji in vivo diketahui bahwa perlakuan kultur cair Streptomyces sp.4 berpengaruh terhadap tingkat kematian larva A. aegypti dan berbeda nyata dengan kontrol negative (p<0.05). Streptomyces sp. 4 diketahui memiliki potensi sebagai salah satu agen larvasida biologis.

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Elkawnie: journal of islamic science and technology

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