沙棘提取物对实验性大鼠结肠炎模型保护作用的研究

IF 0.4 4区农林科学 Q4 VETERINARY SCIENCES

Medycyna Weterynaryjna-Veterinary Medicine-Science and Practice Pub Date : 2023-01-01 DOI:10.21521/mw.6818

MUSTAFA ERKEK, DİLEK AKSIT

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引用次数: 0

摘要

本研究旨在探讨原凸叶(HP)提取物对醋酸诱导大鼠结肠炎模型的促炎细胞因子、氧化应激、细胞凋亡及抗氧化机制的影响。40只Wistar白化大鼠随机分为5组:(1)对照组，(2)HP组，(3)结肠炎组，(4)结肠炎+ HP组，(5)结肠炎+磺胺嘧啶组。检测血液中丙二醛(MDA)、总抗氧化能力(TAS)、超氧化物歧化酶(SOD)、一氧化氮(NO)、白细胞介素-6 (IL-6)、肿瘤坏死因子-α (TNF-α)、铜蓝蛋白和唾液酸水平。此外，对结肠组织进行组织病理学和免疫组织化学检查(b细胞淋巴瘤2 (Bcl-2)和Bcl-2相关X蛋白(Bax))。结肠炎+ HP组与结肠炎组比较，血清MDA、NO、TNF-α、IL-6、唾液酸水平显著降低，血清SOD、TAS、铜蓝蛋白水平显著升高。另一方面，HP可适度减轻组织病理损伤。免疫组织化学检测(Bcl-2, Bax)显示结肠炎组细胞凋亡水平升高，HP应用后细胞凋亡水平降低。HP可改善大鼠结肠炎引起的生化和病理改变。根据本研究得到的结果;HP可能通过抑制促炎细胞因子、细胞凋亡和降低结肠炎的氧化应激而具有保护作用。

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Investigation of protective effects of Harpagophytumprocumbens extract in an experimental rat colitis model

The aim of this study was to investigate the possible effects of Harpagophytum procumbens (HP) extract on pro-inflammatory cytokines, oxidative stress, apoptosis and antioxidant mechanisms in a rat colitis model induced by acetic acid. 40 Wistar albino rats were divided into five equal groups: (1) Control, (2) HP, (3) Colitis, (4) Colitis + HP, (5) Colitis + Sulfasalazine. Malondialdehyde (MDA), total antioxidant capacity (TAS), superoxide dismutase (SOD), nitric oxide (NO), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), ceruloplasmin and sialic acid levels were analyzed in blood samples. In addition colon tissues were evaluated histopathologically and immunohistochemically (B-cell lymphoma 2 (Bcl-2) and Bcl-2 associated X protein (Bax)). A significant decrease in serum MDA, NO, TNF-α, IL-6, sialic acid levels, and an increase in serum SOD, TAS, ceruplasmin levels were observed in Colitis + HP group compared to Colitis group. On the other hand, HP administration moderately reduced histopathological damage. It was detected immunohistochemically (Bcl-2, Bax) that apoptosis levels increased in the Colitis group and decreased with HP application. HP administration ameliorated the biochemical and pathological changes caused by colitis in rats. According to the results obtained in this study; HP might have a protective effect by suppressing of proinflammatory cytokines, apoptosis and reduced oxidative stress in colitis.

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来源期刊

Medycyna Weterynaryjna-Veterinary Medicine-Science and Practice VETERINARY SCIENCES-

CiteScore

0.80

自引率

0.00%

发文量

审稿时长

4-8 weeks

期刊介绍： "Medycyna Weterynaryjna" publishes various types of articles which are grouped in the following editorial categories: reviews, original studies, scientific and professional problems, the history of veterinary medicine, posthumous memoirs, as well as chronicles that briefly relate scientific advances and developments in the veterinary profession and medicine. The most important are the first two categories, which are published with short summaries in English. Moreover, from 2001 the editors of "Medycyna Weterynaryjna", bearing in mind market demands, has also started publishing entire works in English. Since 2008 the periodical has appeared in an electronic version. The following are available in this version: summaries of studies published from 1999 to 2005, full versions of all the studies published in the years 2006-2011 (in pdf files), and full versions of the English studies published in the current year (pdf). Only summaries of the remaining studies from the current year are available. In accordance with the principles accepted by the editors, the full versions of these texts will not be made available until next year. All articles are evaluated twice by leading Polish scientists and professionals before they are considered for publication. For years now "Medycyna Weterynaryjna" has maintained a high standard thanks to this system. The review articles are actually succinct monographs dealing with specific scientific and professional problems that are based on the most recent findings. Original works have a particular value, since they present research carried out in Polish and international scientific centers.