静电纺丝法制备多西环素引导组织再生/骨再生膜治疗种植体周围炎

IF 2.9 4区 医学 Q1 Medicine
Geng Liu, Jianbo Li, Xue Shi
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引用次数: 0

摘要

目的:制备具有抗菌多西环素(DOX)中间层的纳米纤维膜,以指导组织再生并具有抗菌性能。通过使用静电纺丝,我们能够制造dox负载的引导组织再生(GTR)/引导骨再生(GBR)膜,并评估它们在治疗种植体周围炎中的效果。方法:采用静电纺丝法制备含dox的聚己内酯(PCL)纳米纤维,并用扫描电镜(SEM)观察膜的表面形貌。结果:经扫描电镜观察,合成的DOX-PCL纳米纤维表面光滑,直径分布均匀。当DOX的添加量从15%增加到25%时,纤维直径从247.17 nm减小到181.34 nm。差示量热法发现,电纺前PCL和10% PCL电纺膜的吸热峰在66℃时最明显,而DOX粉末的吸热峰在171.5℃时最明显。随着药物装载的变化,碳化发生在223.5摄氏度。PCL在60到66摄氏度之间进行热反应。然而,药物负荷与抑制水平密切相关。琼脂扩散结果表明,负载dox的纳米纤维膜抑制放线菌(Aa)和牙龈卟啉单胞菌的生长;对于这两种细菌,当更多的药物加入到膜上时,抑制带的直径变大。实验1 h、4 h、12 h、1 d、7 d、28 d时,CL+DOX的累积释药率分别为19.14%、36.16%、44.37%、59.52%、65.150%。在试验前三天的最初急剧下降后,PCL-DOX释放率稳定在每小时1.61%左右。最低浓度为3.13 g/mL,而PCL-DOX释放率从第4天的1.28%下降到第28天的0.51%。结论:结合天然高分子材料的可获得性和人造高分子材料的可靠性,采用PCL-GE作为双层面层,PCL-DOX作为中间层制备了三层纳米纤维膜。此外,这些膜的物理绝缘特性得以保持,这使得纤维膜能够执行持续的抗菌作用,并防止药物在组织中最初的突然释放。种植体周围炎可以通过药物和GTR/GBR技术治疗。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Preparation of Doxycycline Hydrochloride Guided Tissue Regeneration/Guided Bone Regeneration Membranes Through Electrostatic Spinning for the Treatment of Peri-Implantitis
Objective: The nanofibre membrane with the antibacterial doxycycline (DOX) intermediate layer can direct tissue regeneration and have antibacterial properties. Through the use of electrospinning, we were able to create DOX-loaded guided tissue regeneration (GTR)/guided bone regeneration (GBR) membranes and assess their efficacy in the treatment of peri-implantitis. Methods: Electrostatic spinning was used to create DOX-containing poly(caprolactone) (PCL) nanofibres, and a scanning electron microscope (SEM) was used to examine the membranes’ surface morphology. Results: The synthesised DOX-PCL nanofibres were found to have a smooth surface and a uniform diameter distribution, as revealed by scanning electron microscopy. When the percentage of DOX was raised from 15% to 25%, the fibre diameter shrank from 247.17 nm to 181.34 nm. According to differential calorimetry, the heat absorption peaks for the pre-electrospun PCL and 10% PCL electrospun membranes were most pronounced at 66 °C, while the peaks for the DOX powder showed at 171.5 °C. With the shift in drug loading, carbonization occurred at 223.5 degrees Celsius. PCL underwent a thermal reaction between 60 and 66 degrees Celsius. However, medication loading was strongly correlated with the level of suppression. Agar diffusion results showed that DOX-loaded nanofibre membranes inhibited the growth of Actinomyces (Aa) and Porphyromonas gingivalis; for both bacterial species, the diameter of the inhibition zone grew larger when more drug was added to the membranes. After 1 hour, 4 hours, 12 hours, 1 day, 7 days, and 28 days of the experiment, the cumulative drug release rates of CL+DOX were 19.14%, 36.16%, 44.37%, 59.52%, and 65.150%, respectively. After the initial steep drop during the first three days of the trial, the PCL-DOX release rate stabilised at around 1.61 percent per hour. Minimum concentration was 3.13 g/mL, while PCL-DOX release rate fell from 1.28 percent on Day 4 to 0.51 percent on Day 28. Conclusion: Combining the availability of natural polymeric materials with the reliability of manufactured polymeric materials, the three-layer nanofibre membranes use PCL-GE as a double surface layer and PCL-DOX as an intermediate layer. In addition, the physical insulating qualities of these membranes are maintained, which allows the fibre membrane to perform a sustained antibacterial action and prevents the initial, abrupt release of the medication in the tissue. Peri-implantitis may be treatable with the use of both pharmaceuticals and GTR/GBR technology.
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来源期刊
CiteScore
4.30
自引率
17.20%
发文量
145
审稿时长
2.3 months
期刊介绍: Information not localized
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