Kunhui He , Yakun Zhang , Wei Ren , Pengyun Chen , Jianchao Liu , Guohua Mi , Fanjun Chen , Qingchun Pan
{"title":"QTL 图谱和转录组分析确定影响玉米水氮相互作用的候选基因","authors":"Kunhui He , Yakun Zhang , Wei Ren , Pengyun Chen , Jianchao Liu , Guohua Mi , Fanjun Chen , Qingchun Pan","doi":"10.1016/j.cj.2023.09.001","DOIUrl":null,"url":null,"abstract":"<div><p>Water and nitrogen fertilization are the key factors limiting maize productivity. The genetic basis of interactions between maize genotype, water, and nitrogen is unclear. A recombinant inbred line (RIL) maize population was evaluated for seven yield and five agronomic traits under four water and nitrogen conditions: water stress and low nitrogen, water stress and high nitrogen, well-watered and low nitrogen, and well-watered and high nitrogen. Respectively eight, six, and six traits varied in response to genotype–water interactions, genotype–nitrogen interactions, and genotype–water–nitrogen interactions. Using a linkage map consisting of 896 single-nucleotide polymorphism markers and multiple-environmental quantitative-trait locus (QTL) mapping, we identified 31 QTL, including 12 for genotype–water–nitrogen interaction, across the four treatments. A set of 8060 genes were differentially expressed among treatments. Integrating genetic analysis, gene co-expression, and functional annotation revealed two candidate genes controlling genotype–water–nitrogen interactions, affecting both leaf width and grain yield. Genes involved in abscisic acid biosynthesis and <em>bZIP</em>, <em>NAC</em>, and <em>WRKY</em> transcription factors participated in maize response to water and nitrogen conditions. These results represent a step toward understanding the genetic regulatory network of maize that responds to water and nitrogen stress and provide a theoretical basis for the genetic improvement of both water- and nitrogen-use efficiency.</p></div>","PeriodicalId":10790,"journal":{"name":"Crop Journal","volume":null,"pages":null},"PeriodicalIF":6.0000,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2214514123001162/pdfft?md5=8ab1f842d78ac23ab71049cb287c7126&pid=1-s2.0-S2214514123001162-main.pdf","citationCount":"0","resultStr":"{\"title\":\"QTL mapping and transcriptome analysis identify candidate genes influencing water–nitrogen interaction in maize\",\"authors\":\"Kunhui He , Yakun Zhang , Wei Ren , Pengyun Chen , Jianchao Liu , Guohua Mi , Fanjun Chen , Qingchun Pan\",\"doi\":\"10.1016/j.cj.2023.09.001\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Water and nitrogen fertilization are the key factors limiting maize productivity. The genetic basis of interactions between maize genotype, water, and nitrogen is unclear. A recombinant inbred line (RIL) maize population was evaluated for seven yield and five agronomic traits under four water and nitrogen conditions: water stress and low nitrogen, water stress and high nitrogen, well-watered and low nitrogen, and well-watered and high nitrogen. Respectively eight, six, and six traits varied in response to genotype–water interactions, genotype–nitrogen interactions, and genotype–water–nitrogen interactions. Using a linkage map consisting of 896 single-nucleotide polymorphism markers and multiple-environmental quantitative-trait locus (QTL) mapping, we identified 31 QTL, including 12 for genotype–water–nitrogen interaction, across the four treatments. A set of 8060 genes were differentially expressed among treatments. Integrating genetic analysis, gene co-expression, and functional annotation revealed two candidate genes controlling genotype–water–nitrogen interactions, affecting both leaf width and grain yield. Genes involved in abscisic acid biosynthesis and <em>bZIP</em>, <em>NAC</em>, and <em>WRKY</em> transcription factors participated in maize response to water and nitrogen conditions. These results represent a step toward understanding the genetic regulatory network of maize that responds to water and nitrogen stress and provide a theoretical basis for the genetic improvement of both water- and nitrogen-use efficiency.</p></div>\",\"PeriodicalId\":10790,\"journal\":{\"name\":\"Crop Journal\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":6.0000,\"publicationDate\":\"2023-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.sciencedirect.com/science/article/pii/S2214514123001162/pdfft?md5=8ab1f842d78ac23ab71049cb287c7126&pid=1-s2.0-S2214514123001162-main.pdf\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Crop Journal\",\"FirstCategoryId\":\"91\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S2214514123001162\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRONOMY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Crop Journal","FirstCategoryId":"91","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2214514123001162","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}
QTL mapping and transcriptome analysis identify candidate genes influencing water–nitrogen interaction in maize
Water and nitrogen fertilization are the key factors limiting maize productivity. The genetic basis of interactions between maize genotype, water, and nitrogen is unclear. A recombinant inbred line (RIL) maize population was evaluated for seven yield and five agronomic traits under four water and nitrogen conditions: water stress and low nitrogen, water stress and high nitrogen, well-watered and low nitrogen, and well-watered and high nitrogen. Respectively eight, six, and six traits varied in response to genotype–water interactions, genotype–nitrogen interactions, and genotype–water–nitrogen interactions. Using a linkage map consisting of 896 single-nucleotide polymorphism markers and multiple-environmental quantitative-trait locus (QTL) mapping, we identified 31 QTL, including 12 for genotype–water–nitrogen interaction, across the four treatments. A set of 8060 genes were differentially expressed among treatments. Integrating genetic analysis, gene co-expression, and functional annotation revealed two candidate genes controlling genotype–water–nitrogen interactions, affecting both leaf width and grain yield. Genes involved in abscisic acid biosynthesis and bZIP, NAC, and WRKY transcription factors participated in maize response to water and nitrogen conditions. These results represent a step toward understanding the genetic regulatory network of maize that responds to water and nitrogen stress and provide a theoretical basis for the genetic improvement of both water- and nitrogen-use efficiency.
Crop JournalAgricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
9.90
自引率
3.00%
发文量
638
审稿时长
41 days
期刊介绍:
The major aims of The Crop Journal are to report recent progresses in crop sciences including crop genetics, breeding, agronomy, crop physiology, germplasm resources, grain chemistry, grain storage and processing, crop management practices, crop biotechnology, and biomathematics.
The regular columns of the journal are Original Research Articles, Reviews, and Research Notes. The strict peer-review procedure will guarantee the academic level and raise the reputation of the journal. The readership of the journal is for crop science researchers, students of agricultural colleges and universities, and persons with similar academic levels.