QTL 图谱和转录组分析确定影响玉米水氮相互作用的候选基因

IF 6 1区 农林科学 Q1 AGRONOMY
Kunhui He , Yakun Zhang , Wei Ren , Pengyun Chen , Jianchao Liu , Guohua Mi , Fanjun Chen , Qingchun Pan
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引用次数: 0

摘要

水和氮肥是限制玉米产量的关键因素。玉米基因型、水和氮之间相互作用的遗传基础尚不清楚。对重组近交系(RIL)玉米群体在四种水氮条件下的七种产量和五种农艺性状进行了评估:水胁迫和低氮、水胁迫和高氮、充足水分和低氮、充足水分和高氮。分别有 8 个、6 个和 6 个性状随基因型-水互作、基因型-氮互作和基因型-水-氮互作而变化。利用由 896 个单核苷酸多态性标记组成的连接图谱和多环境定量性状位点(QTL)图谱,我们在四种处理中发现了 31 个 QTL,包括 12 个基因型-水-氮相互作用的 QTL。不同处理之间有 8060 个基因表达不同。综合遗传分析、基因共表达和功能注释,我们发现了两个控制基因型-水-氮相互作用的候选基因,它们同时影响叶宽和谷粒产量。参与脱落酸生物合成的基因以及bZIP、NAC和WRKY转录因子参与了玉米对水氮条件的响应。这些研究结果为了解玉米应对水和氮胁迫的遗传调控网络迈出了一步,并为遗传改良水和氮的利用效率提供了理论依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
QTL mapping and transcriptome analysis identify candidate genes influencing water–nitrogen interaction in maize

Water and nitrogen fertilization are the key factors limiting maize productivity. The genetic basis of interactions between maize genotype, water, and nitrogen is unclear. A recombinant inbred line (RIL) maize population was evaluated for seven yield and five agronomic traits under four water and nitrogen conditions: water stress and low nitrogen, water stress and high nitrogen, well-watered and low nitrogen, and well-watered and high nitrogen. Respectively eight, six, and six traits varied in response to genotype–water interactions, genotype–nitrogen interactions, and genotype–water–nitrogen interactions. Using a linkage map consisting of 896 single-nucleotide polymorphism markers and multiple-environmental quantitative-trait locus (QTL) mapping, we identified 31 QTL, including 12 for genotype–water–nitrogen interaction, across the four treatments. A set of 8060 genes were differentially expressed among treatments. Integrating genetic analysis, gene co-expression, and functional annotation revealed two candidate genes controlling genotype–water–nitrogen interactions, affecting both leaf width and grain yield. Genes involved in abscisic acid biosynthesis and bZIP, NAC, and WRKY transcription factors participated in maize response to water and nitrogen conditions. These results represent a step toward understanding the genetic regulatory network of maize that responds to water and nitrogen stress and provide a theoretical basis for the genetic improvement of both water- and nitrogen-use efficiency.

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来源期刊
Crop Journal
Crop Journal Agricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
9.90
自引率
3.00%
发文量
638
审稿时长
41 days
期刊介绍: The major aims of The Crop Journal are to report recent progresses in crop sciences including crop genetics, breeding, agronomy, crop physiology, germplasm resources, grain chemistry, grain storage and processing, crop management practices, crop biotechnology, and biomathematics. The regular columns of the journal are Original Research Articles, Reviews, and Research Notes. The strict peer-review procedure will guarantee the academic level and raise the reputation of the journal. The readership of the journal is for crop science researchers, students of agricultural colleges and universities, and persons with similar academic levels.
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