在银屑病小鼠中，LARP7上调SIRT1去乙酰化酶活性，抑制Th1/Th17细胞因子反应

IF 2.5 4区医学 Q3 ALLERGY

Allergologia et immunopathologia Pub Date : 2023-11-01 DOI:10.15586/v51i6.959

None Na Li, Yulei Liu

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引用次数: 0

摘要

目的:通过小鼠模型探讨La核糖核蛋白7 (LARP7)在银屑病中的可能作用并揭示其潜在机制。方法:在C57BL/6小鼠背部皮肤涂抹IMquimod (IMQ)乳膏7 d诱导牛皮癣。免疫印迹法检测SIRT1 (sirtuin家族成员)的去乙酰化酶活性。采用苏木精和伊红染色评价小鼠银屑病的严重程度。流式细胞术检测对Th1/Th17细胞分化的影响。酶联免疫吸附血清学检测分泌细胞因子水平。结果:LARP7上调SIRT1脱乙酰酶活性。LARP7通过上调SIRT1去乙酰化酶活性减轻小鼠牛皮癣症状。此外，LARP7调节银屑病小鼠Th1/Th17细胞分化。我们进一步发现LARP7抑制Th1/Th17细胞因子。结论:LARP7上调银屑病小鼠SIRT1活性，抑制Th1/Th17细胞因子反应。

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LARP7 upregulates SIRT1 deacetylase activity and inhibits Th1/Th17 cytokine response in psoriatic mice

Objective: To investigate the possible role of La ribonucleoprotein 7 (LARP7) in psoriasis through a mouse model and uncover its underlying mechanism. Methods: The back skin of C57BL/6 mice was smeared with IMquimod (IMQ) cream for 7 days to induce psoriasis. Immunoblot kit was used to detect the deacetylase activity of SIRT1 (member of sirtuin family). Hematoxylin and eosin staining was used to assess the degree of psoriasis in mouse. Flow cytometry assays were performed to confirm effects on Th1/Th17 cell differentiation. Enzyme-linked-immunosorbent serologic assays were used to detect the level of secreted cytokines. Results: LARP7 upregulated SIRT1 deacetylase activity. LARP7 alleviated psoriasis symptoms in mice by upregulating SIRT1 deacetylase activity. In addition, LARP7 regulated Th1/Th17 cell differentiation in psoriatic mice. We further found that LARP7 inhibited Th1/Th17 cytokine. Conclusion: LARP7 upregulated SIRT1 activity and inhibited Th1/Th17 cytokine response in psoriatic mice.

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来源期刊

Allergologia et immunopathologia 医学-过敏

CiteScore

3.70

自引率

0.00%

发文量

131

审稿时长

6-12 weeks

期刊介绍： Founded in 1972 by Professor A. Oehling, Allergologia et Immunopathologia is a forum for those working in the field of pediatric asthma, allergy and immunology. Manuscripts related to clinical, epidemiological and experimental allergy and immunopathology related to childhood will be considered for publication. Allergologia et Immunopathologia is the official journal of the Spanish Society of Pediatric Allergy and Clinical Immunology (SEICAP) and also of the Latin American Society of Immunodeficiencies (LASID). It has and independent international Editorial Committee which submits received papers for peer-reviewing by international experts. The journal accepts original and review articles from all over the world, together with consensus statements from the aforementioned societies. Occasionally, the opinion of an expert on a burning topic is published in the "Point of View" section. Letters to the Editor on previously published papers are welcomed. Allergologia et Immunopathologia publishes 6 issues per year and is included in the major databases such as Pubmed, Scopus, Web of Knowledge, etc.