{"title":"葛根素治疗糖尿病骨质疏松的网络药理学机制分析","authors":"Yuwei Chen, Qiyuan Sun","doi":"10.36468/pharmaceutical-sciences.spl.696","DOIUrl":null,"url":null,"abstract":"Puerarin can promote the proliferation of osteoblasts and it can also be used as a scaffold material, but its mechanism is unknown. In this study, we explored the primary molecular mechanism of puerarin in diabetic osteoporosis treatment. We used traditional Chinese medicine systems pharmacology database and analysis platform, PubChem, web-based gene set analysis toolkit, cytoscape and so on to screen the drug targets of puerarin and the disease targets of diabetic osteoporosis. We analyzed the puerarin mechanism based on the protein-protein interaction co-expression network, gene ontology, Kyoto encyclopedia of genes and genomes enrichment, etc., and molecular docking by AutoDock. The main enriched signaling pathways in the Kyoto encyclopedia of genes and genomes were non-small cell lung cancer, endocrine resistance and so on. The main enriched biological processes were positive regulation of cell migration, cytokine-mediated signaling pathway and so on. The main enriched cell components of the cellular component were the integrin complex, platelet alpha granule membrane and so on. The main cellular functions of the molecular function were C-X3-C chemokine binding, nitric oxide synthase regulator activity and so on. The tumor necrosis factor, phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha and prostaglandin-endoperoxide synthase 2 with puerarin docking binding energy were all less than -5 kcal/mol-1. In conclusion puerarin may act through multiple targets and some pathways in diabetic osteoporosis.","PeriodicalId":13292,"journal":{"name":"Indian Journal of Pharmaceutical Sciences","volume":null,"pages":null},"PeriodicalIF":0.4000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Analysis of Puerarin’s Mechanism in Treating Diabetic Osteoporosis by using Network Pharmacology\",\"authors\":\"Yuwei Chen, Qiyuan Sun\",\"doi\":\"10.36468/pharmaceutical-sciences.spl.696\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Puerarin can promote the proliferation of osteoblasts and it can also be used as a scaffold material, but its mechanism is unknown. In this study, we explored the primary molecular mechanism of puerarin in diabetic osteoporosis treatment. We used traditional Chinese medicine systems pharmacology database and analysis platform, PubChem, web-based gene set analysis toolkit, cytoscape and so on to screen the drug targets of puerarin and the disease targets of diabetic osteoporosis. We analyzed the puerarin mechanism based on the protein-protein interaction co-expression network, gene ontology, Kyoto encyclopedia of genes and genomes enrichment, etc., and molecular docking by AutoDock. The main enriched signaling pathways in the Kyoto encyclopedia of genes and genomes were non-small cell lung cancer, endocrine resistance and so on. The main enriched biological processes were positive regulation of cell migration, cytokine-mediated signaling pathway and so on. The main enriched cell components of the cellular component were the integrin complex, platelet alpha granule membrane and so on. The main cellular functions of the molecular function were C-X3-C chemokine binding, nitric oxide synthase regulator activity and so on. The tumor necrosis factor, phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha and prostaglandin-endoperoxide synthase 2 with puerarin docking binding energy were all less than -5 kcal/mol-1. In conclusion puerarin may act through multiple targets and some pathways in diabetic osteoporosis.\",\"PeriodicalId\":13292,\"journal\":{\"name\":\"Indian Journal of Pharmaceutical Sciences\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.4000,\"publicationDate\":\"2023-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Indian Journal of Pharmaceutical Sciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.36468/pharmaceutical-sciences.spl.696\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"PHARMACOLOGY & PHARMACY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Indian Journal of Pharmaceutical Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.36468/pharmaceutical-sciences.spl.696","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}
Analysis of Puerarin’s Mechanism in Treating Diabetic Osteoporosis by using Network Pharmacology
Puerarin can promote the proliferation of osteoblasts and it can also be used as a scaffold material, but its mechanism is unknown. In this study, we explored the primary molecular mechanism of puerarin in diabetic osteoporosis treatment. We used traditional Chinese medicine systems pharmacology database and analysis platform, PubChem, web-based gene set analysis toolkit, cytoscape and so on to screen the drug targets of puerarin and the disease targets of diabetic osteoporosis. We analyzed the puerarin mechanism based on the protein-protein interaction co-expression network, gene ontology, Kyoto encyclopedia of genes and genomes enrichment, etc., and molecular docking by AutoDock. The main enriched signaling pathways in the Kyoto encyclopedia of genes and genomes were non-small cell lung cancer, endocrine resistance and so on. The main enriched biological processes were positive regulation of cell migration, cytokine-mediated signaling pathway and so on. The main enriched cell components of the cellular component were the integrin complex, platelet alpha granule membrane and so on. The main cellular functions of the molecular function were C-X3-C chemokine binding, nitric oxide synthase regulator activity and so on. The tumor necrosis factor, phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha and prostaglandin-endoperoxide synthase 2 with puerarin docking binding energy were all less than -5 kcal/mol-1. In conclusion puerarin may act through multiple targets and some pathways in diabetic osteoporosis.
期刊介绍:
The Indian Journal of Pharmaceutical Sciences (IJPS) is a bi-monthly Journal, which publishes original research work that contributes significantly to further the scientific knowledge in Pharmaceutical Sciences (Pharmaceutical Technology, Pharmaceutics, Biopharmaceutics, Pharmacokinetics, Pharmaceutical/Medicinal Chemistry, Computational Chemistry and Molecular Drug Design, Pharmacognosy and Phytochemistry, Pharmacology and Therapeutics, Pharmaceutical Analysis, Pharmacy Practice, Clinical and Hospital Pharmacy, Pharmacovigilance, Pharmacoepidemiology, Pharmacoeconomics, Drug Information, Patient Counselling, Adverse Drug Reactions Monitoring, Medication Errors, Medication Optimization, Medication Therapy Management, Cell Biology, Genomics and Proteomics, Pharmacogenomics, Bioinformatics and Biotechnology of Pharmaceutical Interest). The Journal publishes original research work either as a Full Research Paper or as a Short Communication. Review Articles on current topics in Pharmaceutical Sciences are also considered for publication by the Journal.