汽车尾气颗粒物的细胞积累改变了BEAS-2B气道上皮细胞溶酶体的酸性pH环境

IF 6.8 Q1 TOXICOLOGY

Journal of Xenobiotics Pub Date : 2023-11-01 DOI:10.3390/jox13040042

Akira Onodera, Takuya Shimomura, Hirohisa Ochi, Ryuto Sunada, Eiko Fukutomi, Koushi Hidaka, Yuichi Kawai

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引用次数: 0

摘要

许多人每天都暴露在汽车尾气颗粒物(vep)中，这些颗粒物被上皮细胞吸收，而上皮细胞是我们生物防御的第一道屏障。这项研究的目的是研究vep是如何在溶酶体降解系统中被处理的。BEAS-2B气道上皮细胞很容易摄取vep，并在细胞中积累数天，但在这段时间内未观察到细胞毒性升高。3D图像分析证实了vep在溶酶体内或溶酶体附近的存在，并且vep的积累导致溶酶体正常酸性pH值的增加和溶酶体酶β-己糖氨酸酶的细胞外释放。上皮细胞被认为激活溶酶体介导的细胞外囊泡分泌，以避免不可降解的外来物质(如vep)造成的损伤，并且作为副反应，溶酶体的酸性pH环境无法维持。

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The Cellular Accumulation of Vehicle Exhaust Particulates Changes the Acidic pH Environment of Lysosomes in BEAS-2B Airway Epithelial Cells

Many people are exposed every day to vehicle exhaust particulates (VEPs), which are thought to be taken up by epithelial cells that are the first barrier in our biological defense. The study aim was to investigate how VEPs are processed in the lysosomal degradation system. BEAS-2B airway epithelial cells easily ingest VEPs and have been shown to accumulate in cells for several days, but no elevated cytotoxicity was observed over that time period. An analysis of 3D images confirmed the presence of VEPs in or near lysosomes, and an accumulation of VEPs resulted in an increase in the normal acidic pH in lysosomes and the extracellular release of the lysosomal enzyme β-hexosaminidase. Epithelial cells were thought to activate the lysosome-mediated secretion of extracellular vesicles to avoid damage caused by non-degradable foreign substances, such as VEPs, and as a side reaction, the acidic pH environment of the lysosomes could not be maintained.

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来源期刊

Journal of Xenobiotics TOXICOLOGY-

CiteScore

5.30

自引率

1.70%

发文量

审稿时长

10 weeks

期刊介绍： The Journal of Xenobiotics publishes original studies concerning the beneficial (pharmacology) and detrimental effects (toxicology) of xenobiotics in all organisms. A xenobiotic (“stranger to life”) is defined as a chemical that is not usually found at significant concentrations or expected to reside for long periods in organisms. In addition to man-made chemicals, natural products could also be of interest if they have potent biological properties, special medicinal properties or that a given organism is at risk of exposure in the environment. Topics dealing with abiotic- and biotic-based transformations in various media (xenobiochemistry) and environmental toxicology are also of interest. Areas of interests include the identification of key physical and chemical properties of molecules that predict biological effects and persistence in the environment; the molecular mode of action of xenobiotics; biochemical and physiological interactions leading to change in organism health; pathophysiological interactions of natural and synthetic chemicals; development of biochemical indicators including new “-omics” approaches to identify biomarkers of exposure or effects for xenobiotics.