{"title":"促红细胞生成素通过抑制氧化应激、炎症和细胞凋亡对大鼠肾缺血再灌注损伤具有保护作用","authors":"Elaf R. Alaasam, Ali M. Janabi","doi":"10.22317/jcms.v9i4.1405","DOIUrl":null,"url":null,"abstract":"Objective: Study investigates erythropoietin's nephroprotective effect in adult male rats with renal ischemia and reperfusion injury, examining biochemical parameters and renal tissue alterations.
 Methods: twenty-eight male rats of Sprague Dawley randomized into four groups: Sham, Iscemia/Reperfusion, NS, and Erythropoietin. Sham group treated kidneys identically without clamping pedicles. Ischemia/Reperfusion group underwent midline laparotomy, 30 minutes ischemia, 2 hours reperfusion, Rats received NS vehicle for Erythropoietin 30 minutes before ischemia, Erythropoietin dose administered 30 minutes before ischemia/reperfusion. Operation performed under maintained anesthesia using ketamine and xylazine injections.
 Results: Renal ischemia /reperfusion increased serum Cr, BUN, IL-1β, NF-kB, Caspase-3, while SOD, GSH, Bcl-2 decreased in induced rats. Erythropoietin treatment reduced Cr and BUN levels, Reduced inflammation, and inflammatory markers. Renal tissue antioxidant markers increased; apoptotic markers decreased. Significant increase in Bcl-2 antiapoptotic marker. Erythropoietin-treated group had significantly better renal histological score compared to induced group.
 Conclusion: Erythropoietin protects against Ischemia/Reperfusion-mediated renal injury, possessing antioxidant, anti-inflammatory, and anti-apoptotic properties.","PeriodicalId":42860,"journal":{"name":"Journal of Contemporary Medical Sciences","volume":"174 1","pages":"0"},"PeriodicalIF":0.2000,"publicationDate":"2023-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Erythropoietin protects against renal ischemia/reperfusion injury in rats via inhibition of oxidative stress, inflammation and apoptosis\",\"authors\":\"Elaf R. Alaasam, Ali M. Janabi\",\"doi\":\"10.22317/jcms.v9i4.1405\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Objective: Study investigates erythropoietin's nephroprotective effect in adult male rats with renal ischemia and reperfusion injury, examining biochemical parameters and renal tissue alterations.
 Methods: twenty-eight male rats of Sprague Dawley randomized into four groups: Sham, Iscemia/Reperfusion, NS, and Erythropoietin. Sham group treated kidneys identically without clamping pedicles. Ischemia/Reperfusion group underwent midline laparotomy, 30 minutes ischemia, 2 hours reperfusion, Rats received NS vehicle for Erythropoietin 30 minutes before ischemia, Erythropoietin dose administered 30 minutes before ischemia/reperfusion. Operation performed under maintained anesthesia using ketamine and xylazine injections.
 Results: Renal ischemia /reperfusion increased serum Cr, BUN, IL-1β, NF-kB, Caspase-3, while SOD, GSH, Bcl-2 decreased in induced rats. Erythropoietin treatment reduced Cr and BUN levels, Reduced inflammation, and inflammatory markers. Renal tissue antioxidant markers increased; apoptotic markers decreased. Significant increase in Bcl-2 antiapoptotic marker. Erythropoietin-treated group had significantly better renal histological score compared to induced group.
 Conclusion: Erythropoietin protects against Ischemia/Reperfusion-mediated renal injury, possessing antioxidant, anti-inflammatory, and anti-apoptotic properties.\",\"PeriodicalId\":42860,\"journal\":{\"name\":\"Journal of Contemporary Medical Sciences\",\"volume\":\"174 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.2000,\"publicationDate\":\"2023-08-26\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Contemporary Medical Sciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.22317/jcms.v9i4.1405\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MEDICINE, GENERAL & INTERNAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Contemporary Medical Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.22317/jcms.v9i4.1405","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MEDICINE, GENERAL & INTERNAL","Score":null,"Total":0}
Erythropoietin protects against renal ischemia/reperfusion injury in rats via inhibition of oxidative stress, inflammation and apoptosis
Objective: Study investigates erythropoietin's nephroprotective effect in adult male rats with renal ischemia and reperfusion injury, examining biochemical parameters and renal tissue alterations.
Methods: twenty-eight male rats of Sprague Dawley randomized into four groups: Sham, Iscemia/Reperfusion, NS, and Erythropoietin. Sham group treated kidneys identically without clamping pedicles. Ischemia/Reperfusion group underwent midline laparotomy, 30 minutes ischemia, 2 hours reperfusion, Rats received NS vehicle for Erythropoietin 30 minutes before ischemia, Erythropoietin dose administered 30 minutes before ischemia/reperfusion. Operation performed under maintained anesthesia using ketamine and xylazine injections.
Results: Renal ischemia /reperfusion increased serum Cr, BUN, IL-1β, NF-kB, Caspase-3, while SOD, GSH, Bcl-2 decreased in induced rats. Erythropoietin treatment reduced Cr and BUN levels, Reduced inflammation, and inflammatory markers. Renal tissue antioxidant markers increased; apoptotic markers decreased. Significant increase in Bcl-2 antiapoptotic marker. Erythropoietin-treated group had significantly better renal histological score compared to induced group.
Conclusion: Erythropoietin protects against Ischemia/Reperfusion-mediated renal injury, possessing antioxidant, anti-inflammatory, and anti-apoptotic properties.
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