初学者指南样品制备技术的透射电子显微镜

IF 0.2 4区生物学 Q4 BIOLOGY

Periodicum Biologorum Pub Date : 2023-10-25 DOI:10.18054/pb.v125i1-2.25293

Ena Dumančić, Lea Vojta, Hrvoje Fulgosi

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引用次数: 0

摘要

背景目的:1930年，随着电子显微镜的发明，显微镜学发生了革命。从那时起，我们可以在超微结构甚至原子水平上研究标本。除了将在本文中描述的透射电子显微镜(TEM)样品制备技术外，还有其他类型的电子显微镜在本文中没有讨论。材料和方法:在这里，我们描述了TEM样品制备的基本步骤，包括组织样品制备，用固定剂对组织进行化学固定，通过速冻进行冷冻固定，用乙醇脱水，用过渡溶剂浸润，树脂包埋和聚合，包埋样品的处理，用超微切片对样品进行切片，样品的阳性和阴性对比，免疫标记和成像。结论:这些方法的收集对透射电镜新手有一定的指导意义。

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Beginners guide to sample preparation techniques for transmission electron microscopy

Background purpose: The revolution in microscopy came in 1930 with the invention of electron microscope. Since then, we can study specimens on ultrastructural and even atomic level. Besides transmission electron microscopy (TEM), for which specimen preparation techniques will be described in this article, there are also other types of electron microscopes that are not discussed in this review. Materials and methods: Here, we have described basic procedures for TEM sample preparation, which include tissue sample preparation, chemical fixation of tissue with fixatives, cryo-fixation performed by quick freezing, dehydration with ethanol, infiltration with transitional solvents, resin embedding and polymerization, processing of embedded specimens, sectioning of samples with ultramicrotome, positive and negative contrasting of samples, immunolabeling, and imaging. Conclusion: Such collection of methods can be useful for novices in transmission electron microscopy.

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来源期刊

Periodicum Biologorum 生物-生物学

CiteScore

0.80

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： This journal provides immediate open access to its content on the principle that making research freely available to the public supports a greater global exchange of knowledge.