{"title":"湖南省不同类型地中海贫血携带者筛查指标的比较","authors":"Hua Tang, Rong Yu, ZiYin Yu, Hui Xi","doi":"10.5937/jomb0-46085","DOIUrl":null,"url":null,"abstract":"Backgroud: Carrier screening is the most effective method to block the occurrence of thalassemia. However, due to differences in race and genotype, MCV, MCH, HbA2 and other indicators are far from each other. The purpose of this study is to evaluate the common screening indicators of α, β and αβ-compound thalassemia carriers in Hunan Province, and try to use the relevant formulas in the existing literature to predict and distinguish different types of thalassemia carriers.
 Methods: Receiver operating characteristic curve (ROC curve) combined with Youden index was utilized to analyze results of blood routine examination, hemoglobin electrophoresis, and literature-related formulas for 1111 α-thalassemia carriers, 464 β-thalassemia carriers and 24 αβ-thalassemia carriers.
 Results: For α-thalassemia carriers, no matter which screening index or formula, the screening efficiency was not ideal. For β-thalassemia minor carriers, RBC, RDW_CV, Hb_A2, Hb_F and formula 5-7 could be used, and for compound thalassemia, RBC, RDW_CV, Hb_A2 and formula 5-6 are suitable. Hb_A2 has high efficiency in the screening of β-thalassemia minor and αβ-thalassemia. For the screening of β-thalassemia minor, if the cut-off value of HbA2 is set to 3%, the detection rate of 93.32% can be obtained at the positive rate of 9.6%, and if it is set to 3.15%, the detection rate can also reach 81.68% at the positive rate of 2.89%. For αβ-thalassemia, if the cut-off value of HbA2 is set to 3%, the detection rate of 95.83% can be obtained under the positive rate of 8.08%.
 Conclusions: Different screening indicators and formulas have different efficiencies for different thalassemia carriers. α-thalassemia carriers are easily missed by screening indicators or corresponding formulas. HbA2 is a better screening indicator for both β-thalassemia minor carriers and αβ-thalassemia carriers, and formulas 5, 6, and 7 are suitable for β-thalassemia minor carriers, and formulas 5 and 6 are better for αβ-thalassemia carriers. To fully and objectively understand each screening index, data support has been provided for clinical and laboratory tests.","PeriodicalId":16175,"journal":{"name":"Journal of Medical Biochemistry","volume":"33 9","pages":"0"},"PeriodicalIF":2.0000,"publicationDate":"2023-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Comparison of screening indicators for different types of thalassemia carriers in Hunan Province\",\"authors\":\"Hua Tang, Rong Yu, ZiYin Yu, Hui Xi\",\"doi\":\"10.5937/jomb0-46085\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Backgroud: Carrier screening is the most effective method to block the occurrence of thalassemia. However, due to differences in race and genotype, MCV, MCH, HbA2 and other indicators are far from each other. The purpose of this study is to evaluate the common screening indicators of α, β and αβ-compound thalassemia carriers in Hunan Province, and try to use the relevant formulas in the existing literature to predict and distinguish different types of thalassemia carriers.
 Methods: Receiver operating characteristic curve (ROC curve) combined with Youden index was utilized to analyze results of blood routine examination, hemoglobin electrophoresis, and literature-related formulas for 1111 α-thalassemia carriers, 464 β-thalassemia carriers and 24 αβ-thalassemia carriers.
 Results: For α-thalassemia carriers, no matter which screening index or formula, the screening efficiency was not ideal. For β-thalassemia minor carriers, RBC, RDW_CV, Hb_A2, Hb_F and formula 5-7 could be used, and for compound thalassemia, RBC, RDW_CV, Hb_A2 and formula 5-6 are suitable. Hb_A2 has high efficiency in the screening of β-thalassemia minor and αβ-thalassemia. For the screening of β-thalassemia minor, if the cut-off value of HbA2 is set to 3%, the detection rate of 93.32% can be obtained at the positive rate of 9.6%, and if it is set to 3.15%, the detection rate can also reach 81.68% at the positive rate of 2.89%. For αβ-thalassemia, if the cut-off value of HbA2 is set to 3%, the detection rate of 95.83% can be obtained under the positive rate of 8.08%.
 Conclusions: Different screening indicators and formulas have different efficiencies for different thalassemia carriers. α-thalassemia carriers are easily missed by screening indicators or corresponding formulas. HbA2 is a better screening indicator for both β-thalassemia minor carriers and αβ-thalassemia carriers, and formulas 5, 6, and 7 are suitable for β-thalassemia minor carriers, and formulas 5 and 6 are better for αβ-thalassemia carriers. To fully and objectively understand each screening index, data support has been provided for clinical and laboratory tests.\",\"PeriodicalId\":16175,\"journal\":{\"name\":\"Journal of Medical Biochemistry\",\"volume\":\"33 9\",\"pages\":\"0\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2023-10-25\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Medical Biochemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5937/jomb0-46085\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Medical Biochemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5937/jomb0-46085","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Comparison of screening indicators for different types of thalassemia carriers in Hunan Province
Backgroud: Carrier screening is the most effective method to block the occurrence of thalassemia. However, due to differences in race and genotype, MCV, MCH, HbA2 and other indicators are far from each other. The purpose of this study is to evaluate the common screening indicators of α, β and αβ-compound thalassemia carriers in Hunan Province, and try to use the relevant formulas in the existing literature to predict and distinguish different types of thalassemia carriers.
Methods: Receiver operating characteristic curve (ROC curve) combined with Youden index was utilized to analyze results of blood routine examination, hemoglobin electrophoresis, and literature-related formulas for 1111 α-thalassemia carriers, 464 β-thalassemia carriers and 24 αβ-thalassemia carriers.
Results: For α-thalassemia carriers, no matter which screening index or formula, the screening efficiency was not ideal. For β-thalassemia minor carriers, RBC, RDW_CV, Hb_A2, Hb_F and formula 5-7 could be used, and for compound thalassemia, RBC, RDW_CV, Hb_A2 and formula 5-6 are suitable. Hb_A2 has high efficiency in the screening of β-thalassemia minor and αβ-thalassemia. For the screening of β-thalassemia minor, if the cut-off value of HbA2 is set to 3%, the detection rate of 93.32% can be obtained at the positive rate of 9.6%, and if it is set to 3.15%, the detection rate can also reach 81.68% at the positive rate of 2.89%. For αβ-thalassemia, if the cut-off value of HbA2 is set to 3%, the detection rate of 95.83% can be obtained under the positive rate of 8.08%.
Conclusions: Different screening indicators and formulas have different efficiencies for different thalassemia carriers. α-thalassemia carriers are easily missed by screening indicators or corresponding formulas. HbA2 is a better screening indicator for both β-thalassemia minor carriers and αβ-thalassemia carriers, and formulas 5, 6, and 7 are suitable for β-thalassemia minor carriers, and formulas 5 and 6 are better for αβ-thalassemia carriers. To fully and objectively understand each screening index, data support has been provided for clinical and laboratory tests.
期刊介绍:
The JOURNAL OF MEDICAL BIOCHEMISTRY (J MED BIOCHEM) is the official journal of the Society of Medical Biochemists of Serbia with international peer-review. Papers are independently reviewed by at least two reviewers selected by the Editors as Blind Peer Reviews. The Journal of Medical Biochemistry is published quarterly.
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