坚果、谷物、豆类和咖啡豆中霉菌毒素的测定以及基于硒的净化处理的有效性

IF 1.9 4区 农林科学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Sana Gammoh, Muhammad H. Alu'datt, Mohammad N. Alhamad, Carole C. Tranchant, Taha Rababah, Khaled Kanakri, Zaid Ammari, Dania Malkawi, Mohammad Alrosan, Thuan-Chew Tan, Haya Alzoubi
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引用次数: 0

摘要

采用液相色谱-串联质谱法(LC-MS/MS)快速定量检测了多个国家的核桃、开心果、花生、咖啡豆、大米和鹰嘴豆中的多种霉菌毒素,特别是黄曲霉毒素 B1、B2、G1 和 G2(AFB1、AFB2、AFG1 和 AFG2)、赭曲霉毒素 A(OTA)、脱氧雪腐镰刀菌烯醇(DON)和玉米赤霉烯酮(ZEN)。此外,还评估了真菌、黄曲霉和寄生曲霉的总计数,以及用无机硒进行净化处理以降低霉菌毒素含量的效果。在检测的 78 个样本中,69%受到霉菌毒素的污染。ZEN 是主要的霉菌毒素污染物,在所有受污染的样本中都检测到了 ZEN,其浓度往往超过最高含量,其次是 AFG1(占受污染样本的 28%)、DON(22%)、AFG2(11%)和 AFB1(5.5%)。黄曲霉毒素的出现与黄曲霉菌和寄生黄曲霉菌的高比例有关。核桃中的 AFB1 和烘焙咖啡豆中的 DON 经水硒处理后可完全去除,而 ZEN 和 AFG1 的含量则根据商品的不同分别降低了 65% 至 89%,烘焙咖啡豆中的含量降低了约 56%。虽然这种新型处理方法在霉菌毒素净化方面大有可为,但并不打算取代上游的安全做法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Determination of mycotoxins in nuts, cereals, legumes, and coffee beans and effectiveness of a selenium-based decontamination treatment

Determination of mycotoxins in nuts, cereals, legumes, and coffee beans and effectiveness of a selenium-based decontamination treatment

Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was used for the rapid quantification of multiple mycotoxins, specifically aflatoxins B1, B2, G1, and G2 (AFB1, AFB2, AFG1, and AFG2), ochratoxin A (OTA), deoxynivalenol (DON), and zearalenone (ZEN), in walnuts, pistachios, peanuts, coffee beans, rice, and chickpeas from various countries. Total counts of fungi, Aspergillus flavus, and Aspergillus parasiticus were also assessed, along with the effectiveness of a decontamination treatment with inorganic selenium to reduce mycotoxin levels. Of the 78 samples tested, 69% were contaminated with mycotoxins. ZEN, the predominant mycotoxin contaminant, was detected in all the contaminated samples in concentrations often exceeding the maximum level, followed by AFG1 (28% of the contaminated samples), DON (22%), AFG2 (11%), and AFB1 (5.5%). The occurrence of aflatoxins was associated with high proportions of A. flavus and A. parasiticus. Complete removal of AFB1 from walnuts and DON from roasted coffee beans was achieved by treatment with aqueous selenium, while the levels of ZEN and AFG1 were respectively lowered by 65% to 89% depending on the commodity and by about 56% in roasted coffee beans. While this novel treatment is a promising approach for mycotoxin decontamination, it is not intended to replace safe practices upstream.

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来源期刊
Journal of Food Safety
Journal of Food Safety 工程技术-生物工程与应用微生物
CiteScore
5.30
自引率
0.00%
发文量
69
审稿时长
1 months
期刊介绍: The Journal of Food Safety emphasizes mechanistic studies involving inhibition, injury, and metabolism of food poisoning microorganisms, as well as the regulation of growth and toxin production in both model systems and complex food substrates. It also focuses on pathogens which cause food-borne illness, helping readers understand the factors affecting the initial detection of parasites, their development, transmission, and methods of control and destruction.
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