半年生作物种子真菌污染的分子鉴定及流行病学研究

IF 1 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Manuel Alfonso Patiño-Moscoso, Karen Viviana Osorio-Guerrero, Deisy Lorena Flórez-Gómez, Luisa Fernanda Sarmiento-Moreno, David Napoleón Vargas-Ramírez
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引用次数: 0

摘要

真菌污染影响种子品质。这些会导致种子流产、腐烂或坏死,以及在生长后期导致疾病发展的幼苗损伤。它们的识别和特征对于建立适当的管理是必不可少的。这项工作的目的是确定并确定在哥伦比亚托利马埃尔埃斯皮纳尔市生产的13种水稻、大豆、玉米和饲用甜高粱植物品种的种子中真菌污染物的流行程度。该研究于2021年第二学期在哥伦比亚农业研究公司AGROSAVIA(哥伦比亚Cundinamarca)的tibaitat研究中心进行。通过吸墨纸试验,鉴定了真菌污染物及其在发芽和未发芽种子中的流行情况。共分离得到65株真菌,经分子鉴定可分为28个形态型(大豆44株,水稻5株,高粱7株,玉米9株)。在未发芽种子中,总流行率最高的属为赤霉属和镰刀菌属。发现的真菌包括镰刀菌(Fusarium spp)、曲霉菌(Curvularia spp)和枯霉菌(Diaporthe spp)等可能的病原菌,以及青霉菌(Penicillium spp)和曲霉菌(Aspergillus spp)等腐生真菌,大豆种子中的赤霉病菌(Fusarium equiseti)和枯霉菌(Diaporthe Diaporthe spp)、水稻种子中的曲霉菌(Curvularia penniseti)、高粱种子中的枯霉菌(Diaporthe melonis)和玉米种子中的黄萎病菌(Fusarium verticillioides)可被认为是对种子萌发能力产生负面影响的潜在种子传播病原体。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Molecular identification and prevalence of fungal contaminants in seeds of semi-annual crops
Seed quality is affected by fungal contamination. These cause abortion, rot, or necrosis of the seed, as well as seedling damage that results in the development of diseases in later stages of growth. Their identification and characterization are essential to establish appropriate management. The objective of this work was to identify and determine the prevalence of fungal contaminants present in the seed of thirteen plant varieties of rice, soybean, corn, and forage sweet sorghum produced in the municipality of El Espinal, Tolima, Colombia. This research was carried out at the Tibaitatá Research Center of the Colombian Agricultural Research Corporation AGROSAVIA (Cundinamarca, Colombia) during the second semester of 2021. By means of a blotter test, fungal contaminants and their prevalence in germinated and non-germinated seeds were identified. A total of 65 fungal isolates were obtained, which were grouped into twenty-eight molecularly identified morphotypes (44 soybean, 5 rice, 7 sorghum and 9 corn). The genera Diaporthe and Fusarium were the ones with the highest total prevalence and in ungerminated seeds. The fungi found include possible pathogens such as Fusarium spp, Curvularia spp and Diaporthe spp. and saprophytic fungi such as Penicillium spp and Aspergillus spp. The isolates of Fusarium equiseti and Diaporthe sp. in soybean seed, Curvularia penniseti in rice seed, Diaporthe melonis in sorghum seed and Fusarium verticillioides in corn seed can be considered as potential seed-borne pathogens that caused a negative effect on germination capacity.
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来源期刊
Scientia Agropecuaria
Scientia Agropecuaria AGRICULTURE, DAIRY & ANIMAL SCIENCE-
CiteScore
3.50
自引率
0.00%
发文量
27
审稿时长
12 weeks
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