毛竹对Wistar白化大鼠体内保护肝的研究

Karisma Borah, Devid Chutia, Manodeep Chakraborty, Ananya Bhattacharjee, Nihar Ranjan Bhuyan
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摘要

肝脏以合成酶、代谢以及药物和食物的排泄而闻名。然而,在生物过程中,肝脏出现异常,成为人类重大的全球健康负担,其特征是合成功能丧失,血液分解,维生素K不规则,以及实质细胞的局部永久性改变。本研究采用对乙酰氨基酚和甲氨蝶呤急性发病模型,对毛竹叶的保肝活性进行植物化学和生物学筛选。本研究使用两种体内模型评估了健康Wistar白化大鼠的肝毒性。每个研究小组由六只动物组成。在第一个模型中,扑热息痛服用了七天。同样,在第二种模型中,以20mg/kg、体重、p.o给药甲氨蝶呤(单剂量处理),两种模型分别以100mg/kg(低)和200 mg/kg(高)p.o给药。第8天取尾静脉血样,分析各项生化指标。在我们的研究中,MESEL成功地恢复了升高的血清生物标志物水平。天门冬氨酸转氨酶的减少是通过去除有毒代谢物引起的,丙氨酸转氨酶的减少是由于线粒体ATP合成的增加,从而调节了肝脏能量代谢的平衡,碱性磷酸盐的减少是由于组织再生,总蛋白的增加表明急性肝损伤后蛋白质失衡的恢复。在不同浓度下,所有这些作用都能增强肝脏,调节身体代谢,并最终抑制进一步的肝细胞损伤,有利于其再生。本研究还证实了MESEL对扑热息痛和甲氨蝶呤模型大鼠的保护作用。该研究揭示了肝细胞再生后肝恢复在临床前设置。关键词:急性肝病,水飞蓟,水飞蓟素,甲氨蝶呤,肝酶
本文章由计算机程序翻译,如有差异,请以英文原文为准。
In-vivo Hepatoprotective Evaluation of Sicyos edulis on Wistar Albino Rats
The liver is known for synthesising enzymes, metabolism, and excretion of drugs and food. However, during biological processes, the abnormality occurs in the liver, which becomes a significant global health burden in humans, characterised by loss of synthetic function, breakdown of blood, irregular vitamin K, and localised, permanent changes to parenchymal cells. The study was designed to research the Phytochemical and biological screening of Sicyos edulis leaf for hepatoprotective activity on laboratory animals using paracetamol and methotrexate model for acute incidence. The study evaluated liver toxicity in healthy Wistar albino rats using two in vivo models. Each study group consists of six animals. In the first model, paracetamol p.o. for seven days. Similarly, in the second model, methotrexate was administered (single dose treatment) to animals with 20mg/kg, b.w., p.o. Both models were challenged with methanolic extract of Sicyos edulis leaf (MESEL) of doses 100mg/kg (low) and 200 mg/kg (high) p.o. for seven days, respectively. On day 8th, the blood samples were collected from the tail vein and analysed for various biochemical parameters. MESEL successfully restored the elevated serum biomarker levels in our study. The decrease in aspartate aminotransferase was observed by removing toxic metabolites, the reduction in alanine aminotransferase was due to an increase in ATP synthesis in mitochondria, thereby modulating the balance of liver energy metabolism, and the decrease in alkaline phosphates is due to tissue regeneration, an increase in total protein denotes the restoration of protein imbalance from acute liver injury. At different concentrations, all these effects strengthen the liver, regulate body metabolism, and ultimately inhibit further liver cell damage in favour of their regeneration. Our study also evidences the protective action of MESEL in rats against the Paracetamol and methotrexate model. The study reveals hepatocyte regeneration followed by hepatic restoration in pre-clinical settings. Keywords: Acute liver disease, Sicyos edulis, Silymarin, Methotrexate, Liver enzymes
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