用于下游分子分析的单个海洋雪粒子采样新方法

IF 2.1 3区 地球科学 Q2 LIMNOLOGY
Chloé M.J. Baumas, Fatima-Ezzahra Ababou, Marc Garel, Mina Bizic, Danny Ionescu, Arthur Puzenat, Frederic A.C. Le Moigne, Hans-Peter Grossart, Christian Tamburini
{"title":"用于下游分子分析的单个海洋雪粒子采样新方法","authors":"Chloé M.J. Baumas,&nbsp;Fatima-Ezzahra Ababou,&nbsp;Marc Garel,&nbsp;Mina Bizic,&nbsp;Danny Ionescu,&nbsp;Arthur Puzenat,&nbsp;Frederic A.C. Le Moigne,&nbsp;Hans-Peter Grossart,&nbsp;Christian Tamburini","doi":"10.1002/lom3.10590","DOIUrl":null,"url":null,"abstract":"<p>The ocean–atmosphere exchange of carbon largely depends on the balance between carbon export of particulate organic carbon (POC) as sinking marine particles, and POC remineralization by attached microbial communities. Despite the vast spectrum of types, sources, ages, shapes, and composition of individual sinking particles, they are usually considered as a bulk together with their associated microbial communities. This limits our mechanistic understanding of the biological carbon pump (BCP) and its feedback on the global carbon cycle. We established a method to sample individual particles while preserving their shape, structure, and nucleic acids by placing a jellified RNA-fixative at the bottom of drifting sediment traps. Coupling imaging of individual particles with associated 16S rRNA analysis reveals that active bacterial communities are highly heterogenous from one particles origin to another. In contrast to lab-made particles, we found that complex in situ conditions lead to heterogeneity even within the same particle type. Our new method allows to associate patterns of active prokaryotic and functional diversity to particle features, enabling the detection of potential remineralization niches. This new approach will therefore improve our understanding of the BCP and numerical representation in the context of a rapidly changing ocean.</p>","PeriodicalId":18145,"journal":{"name":"Limnology and Oceanography: Methods","volume":"22 1","pages":"34-46"},"PeriodicalIF":2.1000,"publicationDate":"2023-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/lom3.10590","citationCount":"0","resultStr":"{\"title\":\"A novel method to sample individual marine snow particles for downstream molecular analyses\",\"authors\":\"Chloé M.J. Baumas,&nbsp;Fatima-Ezzahra Ababou,&nbsp;Marc Garel,&nbsp;Mina Bizic,&nbsp;Danny Ionescu,&nbsp;Arthur Puzenat,&nbsp;Frederic A.C. Le Moigne,&nbsp;Hans-Peter Grossart,&nbsp;Christian Tamburini\",\"doi\":\"10.1002/lom3.10590\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>The ocean–atmosphere exchange of carbon largely depends on the balance between carbon export of particulate organic carbon (POC) as sinking marine particles, and POC remineralization by attached microbial communities. Despite the vast spectrum of types, sources, ages, shapes, and composition of individual sinking particles, they are usually considered as a bulk together with their associated microbial communities. This limits our mechanistic understanding of the biological carbon pump (BCP) and its feedback on the global carbon cycle. We established a method to sample individual particles while preserving their shape, structure, and nucleic acids by placing a jellified RNA-fixative at the bottom of drifting sediment traps. Coupling imaging of individual particles with associated 16S rRNA analysis reveals that active bacterial communities are highly heterogenous from one particles origin to another. In contrast to lab-made particles, we found that complex in situ conditions lead to heterogeneity even within the same particle type. Our new method allows to associate patterns of active prokaryotic and functional diversity to particle features, enabling the detection of potential remineralization niches. This new approach will therefore improve our understanding of the BCP and numerical representation in the context of a rapidly changing ocean.</p>\",\"PeriodicalId\":18145,\"journal\":{\"name\":\"Limnology and Oceanography: Methods\",\"volume\":\"22 1\",\"pages\":\"34-46\"},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2023-11-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://onlinelibrary.wiley.com/doi/epdf/10.1002/lom3.10590\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Limnology and Oceanography: Methods\",\"FirstCategoryId\":\"89\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/lom3.10590\",\"RegionNum\":3,\"RegionCategory\":\"地球科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"LIMNOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Limnology and Oceanography: Methods","FirstCategoryId":"89","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/lom3.10590","RegionNum":3,"RegionCategory":"地球科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"LIMNOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

海洋与大气之间的碳交换在很大程度上取决于作为下沉海洋颗粒的颗粒有机碳(POC)的碳输出与附着微生物群落对颗粒有机碳的再矿化之间的平衡。尽管单个下沉颗粒的类型、来源、年龄、形状和组成千差万别,但它们通常与其相关的微生物群落一起被视为一个整体。这限制了我们对生物碳泵(BCP)及其对全球碳循环反馈的机理理解。我们建立了一种方法,通过在漂流沉积物捕获器底部放置胶状 RNA 固定剂,对单个颗粒进行取样,同时保留其形状、结构和核酸。将单个颗粒的成像与相关的 16S rRNA 分析相结合,可以发现活跃的细菌群落从一个颗粒的起源到另一个颗粒的起源具有高度的异质性。与实验室制造的颗粒不同,我们发现复杂的原位条件甚至会导致同一类型颗粒的异质性。我们的新方法可以将活跃的原核生物和功能多样性模式与颗粒特征联系起来,从而检测潜在的再矿化壁龛。因此,这种新方法将提高我们对瞬息万变的海洋背景下的 BCP 和数字表示法的理解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

A novel method to sample individual marine snow particles for downstream molecular analyses

A novel method to sample individual marine snow particles for downstream molecular analyses

The ocean–atmosphere exchange of carbon largely depends on the balance between carbon export of particulate organic carbon (POC) as sinking marine particles, and POC remineralization by attached microbial communities. Despite the vast spectrum of types, sources, ages, shapes, and composition of individual sinking particles, they are usually considered as a bulk together with their associated microbial communities. This limits our mechanistic understanding of the biological carbon pump (BCP) and its feedback on the global carbon cycle. We established a method to sample individual particles while preserving their shape, structure, and nucleic acids by placing a jellified RNA-fixative at the bottom of drifting sediment traps. Coupling imaging of individual particles with associated 16S rRNA analysis reveals that active bacterial communities are highly heterogenous from one particles origin to another. In contrast to lab-made particles, we found that complex in situ conditions lead to heterogeneity even within the same particle type. Our new method allows to associate patterns of active prokaryotic and functional diversity to particle features, enabling the detection of potential remineralization niches. This new approach will therefore improve our understanding of the BCP and numerical representation in the context of a rapidly changing ocean.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
CiteScore
4.80
自引率
3.70%
发文量
56
审稿时长
3 months
期刊介绍: Limnology and Oceanography: Methods (ISSN 1541-5856) is a companion to ASLO''s top-rated journal Limnology and Oceanography, and articles are held to the same high standards. In order to provide the most rapid publication consistent with high standards, Limnology and Oceanography: Methods appears in electronic format only, and the entire submission and review system is online. Articles are posted as soon as they are accepted and formatted for publication. Limnology and Oceanography: Methods will consider manuscripts whose primary focus is methodological, and that deal with problems in the aquatic sciences. Manuscripts may present new measurement equipment, techniques for analyzing observations or samples, methods for understanding and interpreting information, analyses of metadata to examine the effectiveness of approaches, invited and contributed reviews and syntheses, and techniques for communicating and teaching in the aquatic sciences.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信