高灵敏度零阶发射系统-荧光检测-液相色谱法同时测定瓶装辣椒酱中两种禁用苯基杂蒽染料

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-09-19 DOI:10.4314/bcse.v37i6.2

Hazim M. Ali

{"title":"高灵敏度零阶发射系统-荧光检测-液相色谱法同时测定瓶装辣椒酱中两种禁用苯基杂蒽染料","authors":"Hazim M. Ali","doi":"10.4314/bcse.v37i6.2","DOIUrl":null,"url":null,"abstract":"ABSTRACT. A zero-order emission system was applied to address the variation in emission wavelengths between analytes. Also, it assists to compensate for the drop in emission intensity resulted from the changes in analyte excitation wavelength. So, a high performance liquid chromatography-fluorescence detector (HPLC-FLD) with zero order emission mode was used for the simultaneous estimation of rhodamine B (RhB) and eosin Y (EsY) based on their native fluorescence. The presented method is considered the first fluorescence method for simultaneous determination of RhB and EsY. The efficiency of the developed method was tested by determining the two dyes in a high matrix sample, chili sauce. Under the optimum condition, a linear calibration graphs for RhB and EsY in range of 0.5 -300 ng/mL with r = 0.9994 and 0.9991, respectively, were acquired. The limits of detection for RhB and EsY were 0.344 ng/mL (0.355 ng/g) and 0.215 ng/mL (0.222 ng/g), respectively. The recovery percentage and relative standard deviation of two analytes were obtained in the range of 98.38-101.86% and 0.83-1.66%, respectively. RhB was detected only in one chili sauce sample at a concentration of 0.388 ng/mL while EsY was not found in any sample. KEY WORDS: Rhodamine B, Eosin Y, HPLC-FLD, Zero-order emission mode, Solid phase extraction Bull. Chem. Soc. Ethiop. 2023, 37(6), 1315-1323. DOI: https://dx.doi.org/10.4314/bcse.v37i6.2","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":null,"pages":null},"PeriodicalIF":16.4000,"publicationDate":"2023-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Highly sensitive zero-order emission system - fluorescence detection-liquid chromatographic method for simultaneous estimation of two banned phenyl xanthene dyes in bottled chili sauce\",\"authors\":\"Hazim M. Ali\",\"doi\":\"10.4314/bcse.v37i6.2\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"ABSTRACT. A zero-order emission system was applied to address the variation in emission wavelengths between analytes. Also, it assists to compensate for the drop in emission intensity resulted from the changes in analyte excitation wavelength. So, a high performance liquid chromatography-fluorescence detector (HPLC-FLD) with zero order emission mode was used for the simultaneous estimation of rhodamine B (RhB) and eosin Y (EsY) based on their native fluorescence. The presented method is considered the first fluorescence method for simultaneous determination of RhB and EsY. The efficiency of the developed method was tested by determining the two dyes in a high matrix sample, chili sauce. Under the optimum condition, a linear calibration graphs for RhB and EsY in range of 0.5 -300 ng/mL with r = 0.9994 and 0.9991, respectively, were acquired. The limits of detection for RhB and EsY were 0.344 ng/mL (0.355 ng/g) and 0.215 ng/mL (0.222 ng/g), respectively. The recovery percentage and relative standard deviation of two analytes were obtained in the range of 98.38-101.86% and 0.83-1.66%, respectively. RhB was detected only in one chili sauce sample at a concentration of 0.388 ng/mL while EsY was not found in any sample. KEY WORDS: Rhodamine B, Eosin Y, HPLC-FLD, Zero-order emission mode, Solid phase extraction Bull. Chem. Soc. Ethiop. 2023, 37(6), 1315-1323. DOI: https://dx.doi.org/10.4314/bcse.v37i6.2\",\"PeriodicalId\":1,\"journal\":{\"name\":\"Accounts of Chemical Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":16.4000,\"publicationDate\":\"2023-09-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Accounts of Chemical Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4314/bcse.v37i6.2\",\"RegionNum\":1,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4314/bcse.v37i6.2","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 0

摘要

摘要采用零阶发射系统来处理分析物之间发射波长的变化。此外，它有助于补偿由于分析物激发波长变化而导致的发射强度下降。为此，采用零阶发射模式的高效液相色谱-荧光检测器(HPLC-FLD)，利用罗丹明B (RhB)和伊红Y (EsY)的天然荧光同时测定其含量。该方法被认为是第一个同时测定RhB和EsY的荧光方法。通过对高基质样品辣椒酱中两种染料的测定，验证了该方法的有效性。在最佳条件下，获得了RhB和EsY在0.5 ~ 300 ng/mL范围内的线性校准图，r分别为0.9994和0.9991。RhB和EsY的检出限分别为0.344 ng/mL (0.355 ng/g)和0.215 ng/mL (0.222 ng/g)。两种分析物的回收率和相对标准偏差分别为98.38 ~ 101.86%和0.83 ~ 1.66%。仅在一个辣椒酱样品中检测到RhB，浓度为0.388 ng/mL，而在所有样品中均未检测到EsY。关键词:罗丹明B，伊红Y, HPLC-FLD，零级发射模式，固相萃取公牛。化学。Soc。中国生物医学工程学报，2017,37(6)，1315-1323。DOI: https://dx.doi.org/10.4314/bcse.v37i6.2

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Highly sensitive zero-order emission system - fluorescence detection-liquid chromatographic method for simultaneous estimation of two banned phenyl xanthene dyes in bottled chili sauce

ABSTRACT. A zero-order emission system was applied to address the variation in emission wavelengths between analytes. Also, it assists to compensate for the drop in emission intensity resulted from the changes in analyte excitation wavelength. So, a high performance liquid chromatography-fluorescence detector (HPLC-FLD) with zero order emission mode was used for the simultaneous estimation of rhodamine B (RhB) and eosin Y (EsY) based on their native fluorescence. The presented method is considered the first fluorescence method for simultaneous determination of RhB and EsY. The efficiency of the developed method was tested by determining the two dyes in a high matrix sample, chili sauce. Under the optimum condition, a linear calibration graphs for RhB and EsY in range of 0.5 -300 ng/mL with r = 0.9994 and 0.9991, respectively, were acquired. The limits of detection for RhB and EsY were 0.344 ng/mL (0.355 ng/g) and 0.215 ng/mL (0.222 ng/g), respectively. The recovery percentage and relative standard deviation of two analytes were obtained in the range of 98.38-101.86% and 0.83-1.66%, respectively. RhB was detected only in one chili sauce sample at a concentration of 0.388 ng/mL while EsY was not found in any sample. KEY WORDS: Rhodamine B, Eosin Y, HPLC-FLD, Zero-order emission mode, Solid phase extraction Bull. Chem. Soc. Ethiop. 2023, 37(6), 1315-1323. DOI: https://dx.doi.org/10.4314/bcse.v37i6.2

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.