UTP23是一个重要的预后生物标志物，与乳腺癌的免疫浸润有关

IF 1.5 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Critical Reviews in Eukaryotic Gene Expression Pub Date : 2023-01-01 DOI:10.1615/critreveukaryotgeneexpr.2023048311

Jindong Li, Siman Xie, Benteng Zhang, Weiping He, Yan Zhang, Jun Wang, Li Yang

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引用次数: 0

摘要

背景:乳腺癌是世界范围内妇女发病率高、死亡率高的恶性肿瘤之一，其发病率呈逐年上升趋势，对妇女健康构成严重威胁。UTP23 (UTP23小亚单位加工成分)是核糖体产生所必需的核核蛋白。众所周知，核糖体结构和功能的破坏导致蛋白质功能不正常，影响机体的正常生理过程，促进肿瘤生长。然而，很少有研究表明UTP23与癌症之间存在联系。方法:利用cancer Genome Atlas (TCGA)数据库和Gene expression Omnibus (GEO)数据库分析正常组织和乳腺癌中UTP23 mRNA的表达，利用Human protein Atlas (HPA)数据库分析UTP23蛋白的表达。接下来，我们利用Kaplan-Meier绘图仪检测了UTP23高表达与总生存率(OS)之间的关系，并利用GO/KEGG和GSEA富集了UTP23高表达和低表达样本中的980个差异表达基因，以确定UTP23的潜在生物学功能及其可能影响的信号通路。最后，我们还研究了UTP23与免疫浸润的关系，并通过敲低UTP23检测了UTP23对人乳腺癌细胞系增殖的影响。结果:我们发现乳腺癌患者样本中的UTP23水平明显高于健康个体，并且UTP23水平高与预后不良密切相关(P=0.008)。功能富集分析显示，UTP23的表达与体液免疫应答有关。此外，UTP23表达

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UTP23 is a prominsing prognostic biomarker and is associated with immune infiltration in breast cancer

Background: Breast cancer is one of the malignant tumors with a high incidence and mortality rate among women worldwide, and its prevalence is increasing year by year, posing a serious health risk to women. UTP23 (UTP23 Small Subunit Processome Component) is a nucleolar protein that is essential for ribosome production. As we all know, disruption of ribosome structure and function results in improper protein function, affecting the body's normal physiological processes and promoting cancer growth. However, little research has shown a connection between UTP23 and cancer. Methods: We analyzed the mRNA expression of UTP23 in normal tissue and breast cancer using The Cancer Genome Atlas (TCGA) database and Gene Expression Omnibus (GEO) database, and the protein expression of UTP23 using The Human Protein Atlas (HPA) database. Next, we examined the relationship between UTP23 high expression and Overall Survival (OS) using Kaplan-Meier Plotters and enriched 980 differentially expressed genes in UTP23 high and low expression samples using GO/KEGG and GSEA to identify potential biological functions of UTP23 and signaling pathways that it might influence. Finally, we also investigated the relationship between UTP23 and immune infiltration and examined the effect of UTP23 on the proliferation of human breast cancer cell lines by knocking down UTP23. Results: We found that UTP23 levels in breast cancer patient samples were noticeably greater than those in healthy individuals and that high UTP23 levels were strongly linked with poor prognoses (P=0.008). Functional enrichment analysis revealed that UTP23 expression was connected to the humoral immune response. Besides, UTP23 expres

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来源期刊

Critical Reviews in Eukaryotic Gene Expression 生物-生物工程与应用微生物

CiteScore

2.70

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： Critical ReviewsTM in Eukaryotic Gene Expression presents timely concepts and experimental approaches that are contributing to rapid advances in our mechanistic understanding of gene regulation, organization, and structure within the contexts of biological control and the diagnosis/treatment of disease. The journal provides in-depth critical reviews, on well-defined topics of immediate interest, written by recognized specialists in the field. Extensive literature citations provide a comprehensive information resource. Reviews are developed from an historical perspective and suggest directions that can be anticipated. Strengths as well as limitations of methodologies and experimental strategies are considered.