Innovative processes for the cultivation of mycromycete Fusarium gibbosum CNMN FD 12 and Rhizopus arrhizus CNMN FD 03 – producers of exocellular hydrolases

The optimal concentrations of the heterometallic compounds of “s” and “d” elements Sr, Ca and Fe with polydentate ligands based on 2,6-pyridinedicarboxyl chloride and the Schiff base -2,6-diacetylpyridine bis(picolinoylhydrazone) that increase the synthesis of exocellular proteases and lipases in micromycete Fusarium gibbosum CNMN FD 12 and Rhizopus arrhizus CNMN FD 03 were established. The obtained results were used in the development of 3 optimized mycelial mycelial fungal culture procedures (with alternative variants) that ensure enhanced proteolytic and lipolytic enzyme biosynthesis and shortened culture cycle, which provides opportunities for obtaining indigenous enzyme preparations based on local producing strains characterized by enhanced activity with predicted composition in short periods of time.