Channelrhodopsin-2 螺旋 B 运动的时间分辨 EPR 光谱分析

IF 1.1 4区物理与天体物理 Q4 PHYSICS, ATOMIC, MOLECULAR & CHEMICAL

Applied Magnetic Resonance Pub Date : 2023-09-19 DOI:10.1007/s00723-023-01612-0

Magdalena Schumacher, Christian Bamann, Heinz-Jürgen Steinhoff

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引用次数: 0

摘要

光门控二聚阳离子通道Rhodopsin-2（ChR2）是最重要的光遗传学工具之一。光激活 ChR2 时会发生构象变化，其中最突出的变化包括跨膜螺旋 B 的移动。在本研究中，我们采用时间分辨连续波 EPR 光谱法来跟踪与位于螺旋 B 中 C79 位置结合的自旋标签的光谱变化。我们观察到自旋标签侧链在光照 ChR2 中的运动自由度增加。在黑暗中，由光引起的基本构象变化的恢复与 ChR2 的 P480 状态的恢复相关。因此，观察到的构象变化可能是导致通道对阳离子传导脱敏的关键因素。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Time-Resolved EPR Spectroscopy of Channelrhodopsin-2 Helix B Movements

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Time-Resolved EPR Spectroscopy of Channelrhodopsin-2 Helix B Movements

The light-gated dimeric cation channel channelrhodopsin-2 (ChR2) is one of the most important optogenetic tools. Upon light activation ChR2 undergoes conformational changes, the most prominent ones include a movement of transmembrane helix B. In the present work, we apply time resolved continuous wave EPR spectroscopy to follow spectral changes of a spin label bound to position C79 located in helix B. We observed an increase of the motional freedom of the spin label side chain in illuminated ChR2. The recovery of the underlying light-induced conformational change in the dark is correlated with the recovery of the P480 state of ChR2. The observed conformational changes might be thus key elements responsible for desensitizing the channel for cation conduction.

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来源期刊

Applied Magnetic Resonance 物理-光谱学

CiteScore

1.90

自引率

10.00%

发文量

审稿时长

2.3 months

期刊介绍： Applied Magnetic Resonance provides an international forum for the application of magnetic resonance in physics, chemistry, biology, medicine, geochemistry, ecology, engineering, and related fields. The contents include articles with a strong emphasis on new applications, and on new experimental methods. Additional features include book reviews and Letters to the Editor.