越南球头蛇虫草菌丝体体外生长特性及生物活性研究

IF 0.6 4区环境科学与生态学 Q4 ECOLOGY

Applied Ecology and Environmental Research Pub Date : 2023-01-01 DOI:10.15666/aeer/2105_48974910

T.P.T. DOAN, V.H. NGUYEN, B. SHRESTHA, T.T.H. TRAN, T.T.H. TRAN, T.M. NGUYEN

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IN VITRO GROWTH CHARACTERISTICS AND BIOACTIVITIES OF THE CULTIVATED MYCELIUM OF OPHIOCORDYCEPS SPHECOCEPHALA FROM VIETNAM

. Ophiocordyceps sphecocephala is an entomopathogenic fungus that infects wasps. In this report, the in vitro growth characteristics of Vietnamese Ophiocordyceps sphecocephala collected in Bidoup, Nui Ba, Lam Dong, Vietnam were investigated. The bioactivities of the cultivated mycelium were also studied. The data showed that O. sphecocephala had higher yield of mycelium in mushroom complete medium (MCM), malt yeast medium (MY) and Sabouraud dextrose and yeast extract medium (SDY). Yeast extract and silkworm pupa powder were the preferred nitrogen sources. Besides, O. sphecocephala could utilize various forms of hexoses such as glucose, fructose, sucrose, lactose, maltose, and starch as the carbon sources. The optimal pH for the growth of O. sphecocephala was 6.0. The ethanol extract of O. sphecocephala exhibited several bioactivities: the capacity to scavenge 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical, the inhibitory activity against α-glucosidase and the cytotoxicity against MCF-7 (the breast cancer cell line), HeLa (the cervical cancer cell line), Hepa G2 (the liver cancer cell line), Jurkat (the leukemia cell line) and NCI H460 (the lung cancer cell line) with the highest effect on MCF-7, and the lowest effect on NCI H460. Our finding thus demonstrated a potential of employing O. sphecocephala mycelium in pharmacology.

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来源期刊

Applied Ecology and Environmental Research ECOLOGY-ENVIRONMENTAL SCIENCES

CiteScore

1.40

自引率

14.30%

发文量

104

审稿时长

14 months

期刊介绍： The Journal publishes original research papers and review articles. Researchers from all countries are invited to publish pure or applied ecological, environmental, biogeographical, zoological, botanical, paleontological, biometrical-biomathematical and quantitative ecological or multidisciplinary agricultural research of international interest on its pages. The focus is on topics such as: -Community, ecosystem and global ecology- Biometrics, theoretical- and quantitative ecology- Multidisciplinary agricultural and environmental research- Sustainable and organic agriculture, natural resource management- Ecological methodology, monitoring and modeling- Biodiversity and ecosystem research, microbiology, botany and zoology- Biostatistics and modeling in epidemiology, public health and veterinary- Earth history, paleontology, extinctions, biogeography, biogeochemistry- Conservation biology, environmental protection- Ecological economics, natural capital and ecosystem services- Climatology, meteorology, climate change, climate-ecology. The Journal publishes theoretical papers as well as application-oriented contributions and practical case studies. There is no bias with regard to taxon or geographical area. Purely descriptive papers (like only taxonomic lists) will not be accepted for publication.