{"title":"两个具有成株抗白粉病和高产潜力的小麦-黑麦新品种6R的选育与鉴定","authors":"Guohao Han, Jing Wang, Hanwen Yan, Tiantian Gu, Lijun Cao, Shiyu Liu, Xiuquan Li, Yilin Zhou, Jieru Fan, Zhipeng Shi, Hong Liu, Lihui Li, Diaoguo An","doi":"10.1016/j.cj.2023.09.003","DOIUrl":null,"url":null,"abstract":"Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a devastating disease that seriously threatens wheat yield and quality. To control this disease, host resistance is the most effective measure. Compared with the resistance genes from common wheat, alien resistance genes can better withstand infection of this highly variable pathogen. Development of elite alien germplasm resources with powdery mildew resistance and other key breeding traits is an attractive strategy in wheat breeding. In this study, three wheat-rye germplasm lines YT4-1, YT4-2, and YT4-3 were developed through hybridization between octoploid triticale and common wheat, out of which the lines YT4-1 and YT4-2 conferred adult-plant resistance (APR) to powdery mildew while the line YT4-3 was susceptible to powdery mildew during all of its growth stages. Using genomic in situ hybridization, multi-color fluorescence in situ hybridization, multi-color GISH, and molecular marker analysis, YT4-1, YT4-2, and YT4-3 were shown to be cytogenetically stable wheat-rye 6R addition and T1RS·1BL translocation line, 6RL ditelosomic addition and T1RS·1BL translocation line, and T1RS·1BL translocation line, respectively. Compared with previously reported wheat-rye derivative lines carrying chromosome 6R, YT4-1 and YT4-2 showed stable APR without undesirable pleiotropic effects on agronomic traits. 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引用次数: 0
摘要
小麦白粉病(Blumeria graminis f. sp. tritici, Bgt)是一种严重威胁小麦产量和品质的毁灭性病害。为了控制该病,宿主的抗性是最有效的措施。与普通小麦的抗性基因相比,外来抗性基因能更好地抵御这种高变异病原体的感染。开发具有抗白粉病等关键育种性状的优良外来种质资源是小麦育种的重要策略。本研究通过八倍体小黑麦与普通小麦杂交,获得了3个小麦-黑麦种质系YT4-1、YT4-2和YT4-3,其中YT4-1和YT4-2具有成株抗白粉病能力,而YT4-3在其整个生育期均对白粉病敏感。通过基因组原位杂交、多色荧光原位杂交、多色GISH和分子标记分析,YT4-1、YT4-2和YT4-3分别是细胞遗传稳定的小麦-黑麦6R添加和T1RS·1BL易位系、6RL二染色体添加和T1RS·1BL易位系和T1RS·1BL易位系。与已有报道的携带6R染色体的小麦-黑麦衍生系相比,YT4-1和YT4-2表现出稳定的APR,在农艺性状上没有不良的多效性效应。因此,这些新的小麦-黑麦6R衍生品系有望成为小麦病害育种中有前景的桥梁资源。
Development and identification of two novel wheat-rye 6R derivative lines with adult-plant resistance to powdery mildew and high-yielding potential
Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt), is a devastating disease that seriously threatens wheat yield and quality. To control this disease, host resistance is the most effective measure. Compared with the resistance genes from common wheat, alien resistance genes can better withstand infection of this highly variable pathogen. Development of elite alien germplasm resources with powdery mildew resistance and other key breeding traits is an attractive strategy in wheat breeding. In this study, three wheat-rye germplasm lines YT4-1, YT4-2, and YT4-3 were developed through hybridization between octoploid triticale and common wheat, out of which the lines YT4-1 and YT4-2 conferred adult-plant resistance (APR) to powdery mildew while the line YT4-3 was susceptible to powdery mildew during all of its growth stages. Using genomic in situ hybridization, multi-color fluorescence in situ hybridization, multi-color GISH, and molecular marker analysis, YT4-1, YT4-2, and YT4-3 were shown to be cytogenetically stable wheat-rye 6R addition and T1RS·1BL translocation line, 6RL ditelosomic addition and T1RS·1BL translocation line, and T1RS·1BL translocation line, respectively. Compared with previously reported wheat-rye derivative lines carrying chromosome 6R, YT4-1 and YT4-2 showed stable APR without undesirable pleiotropic effects on agronomic traits. Therefore, these novel wheat-rye 6R derivative lines are expected to be promising bridge resources in wheat disease breeding.
Crop JournalAgricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
9.90
自引率
3.00%
发文量
638
审稿时长
41 days
期刊介绍:
The major aims of The Crop Journal are to report recent progresses in crop sciences including crop genetics, breeding, agronomy, crop physiology, germplasm resources, grain chemistry, grain storage and processing, crop management practices, crop biotechnology, and biomathematics.
The regular columns of the journal are Original Research Articles, Reviews, and Research Notes. The strict peer-review procedure will guarantee the academic level and raise the reputation of the journal. The readership of the journal is for crop science researchers, students of agricultural colleges and universities, and persons with similar academic levels.