转录组和代谢组分析揭示的水稻中生物硝化抑制剂 1,9-癸二醇的潜在分泌转运体和生物合成前体

IF 5.6 2区 农林科学 Q1 AGRONOMY
Di Dongwei , Ma Mingkun , Zhang Xiaoyang , Lu Yufang , Herbert J. Kronzucker , Shi Weiming
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引用次数: 0

摘要

生物硝化抑制剂(BNIs)从植物根部释放,可抑制土壤中微生物的硝化活动,减少 NO3- 沥滤和 N2O 排放,提高氮利用效率(NUE)。最近的几项研究都集中在鉴定新的 BNIs 上,但对控制其生物合成和分泌的基因位点却知之甚少。我们结合转录组学和代谢组学分析,研究了参与 BNI 1,9-癸二醇(1,9-D)生物合成和释放的可能生物合成途径和转运体。我们的研究结果发现,四种脂肪酸(二十碳五烯酸、亚油酸、去甲亚麻酸和多羟基-α,ω-二羧酸)与 1,9-D 的生物合成有关,三个转运体家族(即 ATP 结合盒蛋白家族、多药和有毒化合物挤出家族和主要促进剂超家族)与 1,9-D 从根部释放到土壤介质有关。我们的发现为进一步研究与 1,9-D 的生物合成和分泌有关的基因提供了候选基因,并为作物育种提供了精确的基因位点,通过提高 1,9-D 的分泌来改善氮利用率,从而减少农业土壤中 NO3 的淋失和 N2O 的排放。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Potential Secretory Transporters and Biosynthetic Precursors of Biological Nitrification Inhibitor 1,9-Decanediol in Rice as Revealed by Transcriptome and Metabolome Analyses

Biological nitrification inhibitors (BNIs) are released from plant roots and inhibit the nitrification activity of microorganisms in soils, reducing NO3 leaching and N2O emissions, and increasing nitrogen- use efficiency (NUE). Several recent studies have focused on the identification of new BNIs, yet little is known about the genetic loci that govern their biosynthesis and secretion. We applied a combined transcriptomic and metabolomic analysis to investigate possible biosynthetic pathways and transporters involved in the biosynthesis and release of BNI 1,9-decanediol (1,9-D), which was previously identified in rice root exudates. Our results linked four fatty acids, icosapentaenoic acid, linoleate, norlinolenic acid, and polyhydroxy-α,ω-divarboxylic acid, with 1,9-D biosynthesis and three transporter families, namely the ATP-binding cassette protein family, the multidrug and toxic compound extrusion family, and the major facilitator superfamily, with 1,9-D release from roots into the soil medium. Our finding provided candidates for further work on the genes implicated in the biosynthesis and secretion of 1,9-D and pinpoint genetic loci for crop breeding to improve NUE by enhancing 1,9-D secretion, with the potential to reduce NO3 leaching and N2O emissions from agricultural soils.

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来源期刊
Rice Science
Rice Science Agricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
8.90
自引率
6.20%
发文量
55
审稿时长
40 weeks
期刊介绍: Rice Science is an international research journal sponsored by China National Rice Research Institute. It publishes original research papers, review articles, as well as short communications on all aspects of rice sciences in English language. Some of the topics that may be included in each issue are: breeding and genetics, biotechnology, germplasm resources, crop management, pest management, physiology, soil and fertilizer management, ecology, cereal chemistry and post-harvest processing.
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