发芽致毛霉病的delemrhizopus萌发lncRNA鉴定及加权基因共表达网络分析

IF 4.6 2区 生物学 Q1 MYCOLOGY
Barsha Kalita, Abhijeet Roy, Aiswarya Jayaprakash, Annamalai Arunachalam, Lakshmi P.T.V
{"title":"发芽致毛霉病的delemrhizopus萌发lncRNA鉴定及加权基因共表达网络分析","authors":"Barsha Kalita, Abhijeet Roy, Aiswarya Jayaprakash, Annamalai Arunachalam, Lakshmi P.T.V","doi":"10.1080/21501203.2023.2265414","DOIUrl":null,"url":null,"abstract":"Rhizopus delemar, an opportunistic fungal pathogen, causes a highly fatal disease, mucormycosis. Spore germination is a crucial mechanism for disease pathogenesis. Thus, exploring the molecular mechanisms of fungal germination would underpin our knowledge of such transformation and, in turn, help control mucormycosis. To gain insight into the developmental process particularly associated with cell wall modification and synthesis, weighted gene co-expression network analysis (WGCNA) was performed including both coding and non-coding transcripts identified in the current study, to find out the module of interest in the germination stages. The module-trait relationship identified a particular module to have a high correlation only at the resting phase and further analysis revealed the module to be enriched for protein phosphorylation, carbohydrate metabolic process, and cellular response to stimulus. Moreover, co-expression network analysis of highly connected nodes revealed cell wall modifying enzymes, especially those involved in mannosylation, chitin-glucan crosslinking, and polygalacturonase activities co-expressing and interacting with the novel lncRNAs among which some of them predicted to be endogenous target mimic (eTM) lncRNAs. Hence, the present study provides an insight into the onset of spore germination and the information on the novel non-coding transcripts with key cell wall–related enzymes as potential targets against mucormycosis.","PeriodicalId":18833,"journal":{"name":"Mycology","volume":"51 31","pages":"0"},"PeriodicalIF":4.6000,"publicationDate":"2023-11-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Identification of lncRNA and weighted gene coexpression network analysis of germinating <i>Rhizopus delemar</i> causing mucormycosis\",\"authors\":\"Barsha Kalita, Abhijeet Roy, Aiswarya Jayaprakash, Annamalai Arunachalam, Lakshmi P.T.V\",\"doi\":\"10.1080/21501203.2023.2265414\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Rhizopus delemar, an opportunistic fungal pathogen, causes a highly fatal disease, mucormycosis. Spore germination is a crucial mechanism for disease pathogenesis. Thus, exploring the molecular mechanisms of fungal germination would underpin our knowledge of such transformation and, in turn, help control mucormycosis. To gain insight into the developmental process particularly associated with cell wall modification and synthesis, weighted gene co-expression network analysis (WGCNA) was performed including both coding and non-coding transcripts identified in the current study, to find out the module of interest in the germination stages. The module-trait relationship identified a particular module to have a high correlation only at the resting phase and further analysis revealed the module to be enriched for protein phosphorylation, carbohydrate metabolic process, and cellular response to stimulus. Moreover, co-expression network analysis of highly connected nodes revealed cell wall modifying enzymes, especially those involved in mannosylation, chitin-glucan crosslinking, and polygalacturonase activities co-expressing and interacting with the novel lncRNAs among which some of them predicted to be endogenous target mimic (eTM) lncRNAs. Hence, the present study provides an insight into the onset of spore germination and the information on the novel non-coding transcripts with key cell wall–related enzymes as potential targets against mucormycosis.\",\"PeriodicalId\":18833,\"journal\":{\"name\":\"Mycology\",\"volume\":\"51 31\",\"pages\":\"0\"},\"PeriodicalIF\":4.6000,\"publicationDate\":\"2023-11-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Mycology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1080/21501203.2023.2265414\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"MYCOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Mycology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1080/21501203.2023.2265414","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"MYCOLOGY","Score":null,"Total":0}
引用次数: 0

摘要

delemar根霉是一种机会性真菌病原体,引起一种高度致命的疾病——毛霉病。孢子萌发是疾病发病的重要机制。因此,探索真菌萌发的分子机制将巩固我们对这种转化的认识,进而有助于控制毛霉病。为了深入了解与细胞壁修饰和合成相关的发育过程,我们进行了加权基因共表达网络分析(WGCNA),包括当前研究中鉴定的编码和非编码转录本,以找出萌发阶段感兴趣的模块。模块-性状关系确定了一个特定模块仅在静息期具有高相关性,进一步分析表明该模块在蛋白质磷酸化、碳水化合物代谢过程和细胞对刺激的反应中富集。此外,高连接节点的共表达网络分析显示,细胞壁修饰酶,特别是涉及甘露糖基化,几丁质-葡聚糖交联和聚半乳糖醛酸酶活性的酶,与新型lncRNAs共表达和相互作用,其中一些被预测为内源性靶模拟(eTM) lncRNAs。因此,本研究提供了孢子萌发的开始和新的非编码转录物的信息,关键细胞壁相关酶作为毛霉病的潜在靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Identification of lncRNA and weighted gene coexpression network analysis of germinating Rhizopus delemar causing mucormycosis
Rhizopus delemar, an opportunistic fungal pathogen, causes a highly fatal disease, mucormycosis. Spore germination is a crucial mechanism for disease pathogenesis. Thus, exploring the molecular mechanisms of fungal germination would underpin our knowledge of such transformation and, in turn, help control mucormycosis. To gain insight into the developmental process particularly associated with cell wall modification and synthesis, weighted gene co-expression network analysis (WGCNA) was performed including both coding and non-coding transcripts identified in the current study, to find out the module of interest in the germination stages. The module-trait relationship identified a particular module to have a high correlation only at the resting phase and further analysis revealed the module to be enriched for protein phosphorylation, carbohydrate metabolic process, and cellular response to stimulus. Moreover, co-expression network analysis of highly connected nodes revealed cell wall modifying enzymes, especially those involved in mannosylation, chitin-glucan crosslinking, and polygalacturonase activities co-expressing and interacting with the novel lncRNAs among which some of them predicted to be endogenous target mimic (eTM) lncRNAs. Hence, the present study provides an insight into the onset of spore germination and the information on the novel non-coding transcripts with key cell wall–related enzymes as potential targets against mucormycosis.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Mycology
Mycology Medicine-Infectious Diseases
CiteScore
9.10
自引率
0.00%
发文量
18
审稿时长
13 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信