单月果酸甘油通过抑制脂多糖暴露鸡胚胎的炎症反应和核因子κ b信号传导,改善肠道形态和抗氧化状态

IF 6.3
Linglian Kong , Yuanli Cai , Xue Pan , Chuanpi Xiao , Zhigang Song
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引用次数: 0

摘要

中链脂肪酸及其衍生物是支持动物免疫功能的天然成分。采用鸡胚模型研究了单月桂酸甘油(GML)对肠道先天免疫的影响及其分子机制。选用64只爱拔益加肉仔鸡胚胎,随机分为4组。在胚期17.5 d,饲喂9 mg GML,孵育12 h,再用32 μg脂多糖(LPS)攻毒12 h。胚胎第18.5天,取空肠和回肠。结果表明,GML逆转了lps诱导的绒毛高度下降,上调了粘蛋白2 (mucin 2, P <0.05)。GML降低lps诱导的丙二醛生成,提高抗氧化酶活性(P <0.05)。GML减轻lps刺激的肠道分泌白细胞介素(IL)-1β、IL-6和肿瘤坏死因子-α (TNF-α) (P <0.05)。GML还使lps诱导的toll样受体4、核因子κ b p65 (NF-κB p65)、环氧化酶-2、nod样受体蛋白3、IL-18、闭塞带1和occludin (P <0.05)。GML还增强了amp活化蛋白激酶α1和claudin 1 (P <0.05)。综上所述,GML通过调节NF-κB信号通路和减轻lps致伤肉鸡胚胎的炎症反应,改善了肠道形态和抗氧化状态。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Glycerol monolaurate improves intestinal morphology and antioxidant status by suppressing inflammatory responses and nuclear factor kappa-B signaling in lipopolysaccharide-exposed chicken embryos

Medium-chain fatty acids and their derivatives are natural ingredients that support immunological functions in animals. The effects of glycerol monolaurate (GML) on intestinal innate immunity and associated molecular mechanisms were investigated using a chicken embryo model. Sixty-four Arbor Acres broiler embryos were randomly allocated into four groups. On embryonic day 17.5, the broiler embryos were administered with 9 mg of GML, which was followed by a 12-h incubation period and a 12-h challenge with 32 μg of lipopolysaccharide (LPS). On embryonic day 18.5, the jejunum and ileum were harvested. Results indicated that GML reversed the LPS-induced decline in villus height and upregulated the expression of mucin 2 (P < 0.05). GML decreased LPS-induced malondialdehyde production and boosted antioxidant enzyme activity (P < 0.05). GML alleviated LPS-stimulated intestinal secretion of interleukin (IL)-1β, IL-6, and tumor necrosis factor-α (TNF-α) (P < 0.05). GML also normalized LPS-induced changes in the gene expression of Toll-like receptor 4, nuclear factor kappa-B p65 (NF-κB p65), cyclooxygenase-2, NOD-like receptor protein 3, IL-18, zonula occludens 1, and occludin (P < 0.05). GML enhanced as well the expression of AMP-activated protein kinase α1 and claudin 1 (P < 0.05). In conclusion, GML improved intestinal morphology and antioxidant status by alleviating inflammatory responses and modulating NF-κB signaling in LPS-challenged broiler embryos.

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来源期刊
Animal Nutrition
Animal Nutrition Animal Science and Zoology
CiteScore
9.70
自引率
0.00%
发文量
542
审稿时长
65 days
期刊介绍: Animal Nutrition encompasses the full gamut of animal nutritional sciences and reviews including, but not limited to, fundamental aspects of animal nutrition such as nutritional requirements, metabolic studies, body composition, energetics, immunology, neuroscience, microbiology, genetics and molecular and cell biology related to primarily to the nutrition of farm animals and aquatic species. More applied aspects of animal nutrition, such as the evaluation of novel ingredients, feed additives and feed safety will also be considered but it is expected that such studies will have a strong nutritional focus. Animal Nutrition is indexed in SCIE, PubMed Central, Scopus, DOAJ, etc.
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