Gamze Tilbe İnce, Mehmet Yüksekkaya, Orhan Erdem Haberal
{"title":"微聚合酶链反应用于即时检测及其他:微流体和纳米流体的综述","authors":"Gamze Tilbe İnce, Mehmet Yüksekkaya, Orhan Erdem Haberal","doi":"10.1007/s10404-023-02677-w","DOIUrl":null,"url":null,"abstract":"<div><p>Miniaturization of biological and chemical analytical devices with microelectromechanical systems (MEMS) technology is important for medical diagnosis, microbial recognition, and other biological analysis. Current or emerging infectious diseases increase the need for point-of-care testing (POCT) to increase timely diagnosis and treatment. Among the various nucleic acid amplification methods, polymerase chain reaction (PCR) has been the most used method due to its simplicity. MEMS and microfluidic technologies enable PCR processes to be miniaturized in a chip. A miniaturized microfluidic chip is a small device that can limit and flow a specific volume of fluid into micro-sized channels. Microfluidic chip has potential benefits such as speed, cost, portability, efficiency, and automation. In addition to these benefits, multifunctional POCT PCR devices based on microfluidics technology can help with clinical diagnosis in underdeveloped nations that need a centralized health care system. In this review, conventional PCR method and the recent advances in microfluidic PCR amplification technologies, including its usage for POCT, are discussed. Current studies in commercialization of microfluidic PCR devices are presented. POCT has become very important during the Coronavirus disease (COVID-19) epidemic; therefore, the applications that evolved with combined use of POCT, PCR, and microfluidics during the COVID-19 pandemic were also covered.</p></div>","PeriodicalId":706,"journal":{"name":"Microfluidics and Nanofluidics","volume":null,"pages":null},"PeriodicalIF":2.3000,"publicationDate":"2023-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Micro-polymerase chain reaction for point-of-care detection and beyond: a review microfluidics and nanofluidics\",\"authors\":\"Gamze Tilbe İnce, Mehmet Yüksekkaya, Orhan Erdem Haberal\",\"doi\":\"10.1007/s10404-023-02677-w\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Miniaturization of biological and chemical analytical devices with microelectromechanical systems (MEMS) technology is important for medical diagnosis, microbial recognition, and other biological analysis. Current or emerging infectious diseases increase the need for point-of-care testing (POCT) to increase timely diagnosis and treatment. Among the various nucleic acid amplification methods, polymerase chain reaction (PCR) has been the most used method due to its simplicity. MEMS and microfluidic technologies enable PCR processes to be miniaturized in a chip. A miniaturized microfluidic chip is a small device that can limit and flow a specific volume of fluid into micro-sized channels. Microfluidic chip has potential benefits such as speed, cost, portability, efficiency, and automation. In addition to these benefits, multifunctional POCT PCR devices based on microfluidics technology can help with clinical diagnosis in underdeveloped nations that need a centralized health care system. In this review, conventional PCR method and the recent advances in microfluidic PCR amplification technologies, including its usage for POCT, are discussed. Current studies in commercialization of microfluidic PCR devices are presented. POCT has become very important during the Coronavirus disease (COVID-19) epidemic; therefore, the applications that evolved with combined use of POCT, PCR, and microfluidics during the COVID-19 pandemic were also covered.</p></div>\",\"PeriodicalId\":706,\"journal\":{\"name\":\"Microfluidics and Nanofluidics\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2023-09-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microfluidics and Nanofluidics\",\"FirstCategoryId\":\"5\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s10404-023-02677-w\",\"RegionNum\":4,\"RegionCategory\":\"工程技术\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"INSTRUMENTS & INSTRUMENTATION\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microfluidics and Nanofluidics","FirstCategoryId":"5","ListUrlMain":"https://link.springer.com/article/10.1007/s10404-023-02677-w","RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"INSTRUMENTS & INSTRUMENTATION","Score":null,"Total":0}
Micro-polymerase chain reaction for point-of-care detection and beyond: a review microfluidics and nanofluidics
Miniaturization of biological and chemical analytical devices with microelectromechanical systems (MEMS) technology is important for medical diagnosis, microbial recognition, and other biological analysis. Current or emerging infectious diseases increase the need for point-of-care testing (POCT) to increase timely diagnosis and treatment. Among the various nucleic acid amplification methods, polymerase chain reaction (PCR) has been the most used method due to its simplicity. MEMS and microfluidic technologies enable PCR processes to be miniaturized in a chip. A miniaturized microfluidic chip is a small device that can limit and flow a specific volume of fluid into micro-sized channels. Microfluidic chip has potential benefits such as speed, cost, portability, efficiency, and automation. In addition to these benefits, multifunctional POCT PCR devices based on microfluidics technology can help with clinical diagnosis in underdeveloped nations that need a centralized health care system. In this review, conventional PCR method and the recent advances in microfluidic PCR amplification technologies, including its usage for POCT, are discussed. Current studies in commercialization of microfluidic PCR devices are presented. POCT has become very important during the Coronavirus disease (COVID-19) epidemic; therefore, the applications that evolved with combined use of POCT, PCR, and microfluidics during the COVID-19 pandemic were also covered.
期刊介绍:
Microfluidics and Nanofluidics is an international peer-reviewed journal that aims to publish papers in all aspects of microfluidics, nanofluidics and lab-on-a-chip science and technology. The objectives of the journal are to (1) provide an overview of the current state of the research and development in microfluidics, nanofluidics and lab-on-a-chip devices, (2) improve the fundamental understanding of microfluidic and nanofluidic phenomena, and (3) discuss applications of microfluidics, nanofluidics and lab-on-a-chip devices. Topics covered in this journal include:
1.000 Fundamental principles of micro- and nanoscale phenomena like,
flow, mass transport and reactions
3.000 Theoretical models and numerical simulation with experimental and/or analytical proof
4.000 Novel measurement & characterization technologies
5.000 Devices (actuators and sensors)
6.000 New unit-operations for dedicated microfluidic platforms
7.000 Lab-on-a-Chip applications
8.000 Microfabrication technologies and materials
Please note, Microfluidics and Nanofluidics does not publish manuscripts studying pure microscale heat transfer since there are many journals that cover this field of research (Journal of Heat Transfer, Journal of Heat and Mass Transfer, Journal of Heat and Fluid Flow, etc.).