微聚合酶链反应用于即时检测及其他:微流体和纳米流体的综述

IF 2.3 4区 工程技术 Q2 INSTRUMENTS & INSTRUMENTATION
Gamze Tilbe İnce, Mehmet Yüksekkaya, Orhan Erdem Haberal
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引用次数: 0

摘要

采用微机电系统(MEMS)技术的生物和化学分析设备的小型化对于医学诊断、微生物识别和其他生物分析非常重要。当前或新出现的传染病增加了对护理点检测(POCT)的需求,以增加及时诊断和治疗。在各种核酸扩增方法中,聚合酶链反应(PCR)因其简便而成为应用最多的方法。MEMS和微流体技术使PCR过程在芯片中小型化。微型微流控芯片是一种可以限制特定体积的流体流入微尺寸通道的小型装置。微流控芯片具有速度快、成本低、便携、高效、自动化等优点。除了这些好处之外,基于微流体技术的多功能POCT PCR装置可以帮助需要集中医疗保健系统的不发达国家进行临床诊断。本文综述了传统PCR方法和微流控PCR扩增技术的最新进展,包括其在POCT中的应用。介绍了目前微流控PCR装置商业化的研究情况。POCT在冠状病毒病(COVID-19)流行期间变得非常重要;因此,在COVID-19大流行期间,POCT、PCR和微流控技术的结合应用也被涵盖。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Micro-polymerase chain reaction for point-of-care detection and beyond: a review microfluidics and nanofluidics

Micro-polymerase chain reaction for point-of-care detection and beyond: a review microfluidics and nanofluidics

Miniaturization of biological and chemical analytical devices with microelectromechanical systems (MEMS) technology is important for medical diagnosis, microbial recognition, and other biological analysis. Current or emerging infectious diseases increase the need for point-of-care testing (POCT) to increase timely diagnosis and treatment. Among the various nucleic acid amplification methods, polymerase chain reaction (PCR) has been the most used method due to its simplicity. MEMS and microfluidic technologies enable PCR processes to be miniaturized in a chip. A miniaturized microfluidic chip is a small device that can limit and flow a specific volume of fluid into micro-sized channels. Microfluidic chip has potential benefits such as speed, cost, portability, efficiency, and automation. In addition to these benefits, multifunctional POCT PCR devices based on microfluidics technology can help with clinical diagnosis in underdeveloped nations that need a centralized health care system. In this review, conventional PCR method and the recent advances in microfluidic PCR amplification technologies, including its usage for POCT, are discussed. Current studies in commercialization of microfluidic PCR devices are presented. POCT has become very important during the Coronavirus disease (COVID-19) epidemic; therefore, the applications that evolved with combined use of POCT, PCR, and microfluidics during the COVID-19 pandemic were also covered.

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来源期刊
Microfluidics and Nanofluidics
Microfluidics and Nanofluidics 工程技术-纳米科技
CiteScore
4.80
自引率
3.60%
发文量
97
审稿时长
2 months
期刊介绍: Microfluidics and Nanofluidics is an international peer-reviewed journal that aims to publish papers in all aspects of microfluidics, nanofluidics and lab-on-a-chip science and technology. The objectives of the journal are to (1) provide an overview of the current state of the research and development in microfluidics, nanofluidics and lab-on-a-chip devices, (2) improve the fundamental understanding of microfluidic and nanofluidic phenomena, and (3) discuss applications of microfluidics, nanofluidics and lab-on-a-chip devices. Topics covered in this journal include: 1.000 Fundamental principles of micro- and nanoscale phenomena like, flow, mass transport and reactions 3.000 Theoretical models and numerical simulation with experimental and/or analytical proof 4.000 Novel measurement & characterization technologies 5.000 Devices (actuators and sensors) 6.000 New unit-operations for dedicated microfluidic platforms 7.000 Lab-on-a-Chip applications 8.000 Microfabrication technologies and materials Please note, Microfluidics and Nanofluidics does not publish manuscripts studying pure microscale heat transfer since there are many journals that cover this field of research (Journal of Heat Transfer, Journal of Heat and Mass Transfer, Journal of Heat and Fluid Flow, etc.).
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