植物黄萎病毒力的DNA甲基化表观遗传调控。

IF 4.6 4区 农林科学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Yun-Ya Chen, Chen Zhu, Jian-Hua Zhao, Ting Liu, Feng Gao, Ying-Chao Zhang, Cheng-Guo Duan
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引用次数: 0

摘要

DNA胞嘧啶甲基化作为一种保守的表观遗传标记,在5'位置(5- mc)起着重要的作用,在包括植物免疫在内的多个生物过程中发挥着重要作用。然而,DNA甲基化在植物致病真菌毒力决定因素中的作用仍然难以捉摸。在这项研究中,我们分析了植物病原真菌黄萎病(Verticillium dahliae)的DNA甲基化模式,并探讨了其在真菌致病性中的作用。黄萎病是造成许多作物损失的主要病原菌之一。我们发现DNA甲基化修饰存在于大丽花中,并且是其在宿主植物中完全毒力所必需的。鉴定了大丽花DNA甲基化的主要酶。我们提供的证据表明,DNA甲基化酶介导的DNA甲基化模式的建立正调节真菌毒力,主要通过抑制一个保守的蛋白激酶vdrim15介导的Ca2+信号和ROS的产生,这是大丽花的渗透活性所必需的。此外,我们进一步证明了真核生物中与5-mC密切相关的另一种异染色质标记H3赖氨酸9三甲基化(H3K9me3)也通过类似的机制参与了大丽花致病性的调控。更重要的是,DNA甲基转移酶基因VdRid、VdDnmt5以及H3K9me3甲基转移酶基因在感染早期被大量诱导,这意味着有效感染需要对5-mC和H3K9me3稳态进行动态调控。总的来说,我们的发现揭示了植物致病性真菌毒力调控的表观遗传机制。补充信息:在线版本包含补充资料,下载地址:10.1007/s42994-023-00117-5。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
DNA methylation-dependent epigenetic regulation of Verticillium dahliae virulence in plants

As a conserved epigenetic mark, DNA cytosine methylation, at the 5’ position (5-mC), plays important roles in multiple biological processes, including plant immunity. However, the involvement of DNA methylation in the determinants of virulence of phytopathogenic fungi remains elusive. In this study, we profiled the DNA methylation patterns of the phytopathogenic fungus Verticillium dahliae, one of the major causal pathogens of Verticillium wilt disease that causes great losses in many crops, and explored its contribution in fungal pathogenicity. We reveal that DNA methylation modification is present in V. dahliae and is required for its full virulence in host plants. The major enzymes responsible for the establishment of DNA methylation in V. dahliae were identified. We provided evidence that DNA methyltransferase-mediated establishment of DNA methylation pattern positively regulates fungal virulence, mainly through repressing a conserved protein kinase VdRim15-mediated Ca2+ signaling and ROS production, which is essential for the penetration activity of V. dahliae. In addition, we further demonstrated that histone H3 lysine 9 trimethylation (H3K9me3), another heterochromatin marker that is closely associated with 5-mC in eukaryotes, also participates in the regulation of V. dahliae pathogenicity, through a similar mechanism. More importantly, DNA methyltransferase genes VdRid, VdDnmt5, as well as H3K9me3 methyltransferase genes, were greatly induced during the early infection phase, implying that a dynamic regulation of 5-mC and H3K9me3 homeostasis is required for an efficient infection. Collectively, our findings uncover an epigenetic mechanism in the regulation of phytopathogenic fungal virulence.

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来源期刊
CiteScore
7.70
自引率
2.80%
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