Adara Barbosa de Sousa, Paula Rohr, Henrique C S Silveira
{"title":"巴西职业农药暴露农民线粒体DNA拷贝数变异分析。","authors":"Adara Barbosa de Sousa, Paula Rohr, Henrique C S Silveira","doi":"10.1080/09603123.2023.2280147","DOIUrl":null,"url":null,"abstract":"<p><p>The use of pesticide use has been linked to the higher production of reactive oxygen species, resulting in oxidative stress, which in turn can cause genomic instability. A marker for instability is the copy number variation of the mitochondrial genome (mtDNAcn), which has been found to be altered in diverse human diseases, including tumors. This research aimed to examine the variation of mtDNAcn in individuals occupationally exposed to pesticides. Real-time PCR assays were conducted on 154 individuals (78 exposed and 76 non-exposed). Pesticide-exposed ndividuals exhibited a significant reduction in mtDNAcn (1.11 ± 0.37mtDNAcn/genome) compared to non-exposed individuals (1.30 ± 0.33mtDNAcn/genome; <i>p</i> = 0.001). The multivariate analysis indicated that individuals who reported using haloxyfop and copper sulfate demonstrated an increase (β = 0.200, <i>p</i> = 0.053) and a decrease (β=-0.2, <i>p</i> = 0.021), respectively, in mtDNAcn. In conclusion, our findings suggest that chronic exposure to pesticides results in changes in mtDNAcn.</p>","PeriodicalId":14039,"journal":{"name":"International Journal of Environmental Health Research","volume":null,"pages":null},"PeriodicalIF":2.2000,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Analysis of mitochondrial DNA copy number variation in Brazilian farmers occupationally exposed to pesticides.\",\"authors\":\"Adara Barbosa de Sousa, Paula Rohr, Henrique C S Silveira\",\"doi\":\"10.1080/09603123.2023.2280147\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The use of pesticide use has been linked to the higher production of reactive oxygen species, resulting in oxidative stress, which in turn can cause genomic instability. A marker for instability is the copy number variation of the mitochondrial genome (mtDNAcn), which has been found to be altered in diverse human diseases, including tumors. This research aimed to examine the variation of mtDNAcn in individuals occupationally exposed to pesticides. Real-time PCR assays were conducted on 154 individuals (78 exposed and 76 non-exposed). Pesticide-exposed ndividuals exhibited a significant reduction in mtDNAcn (1.11 ± 0.37mtDNAcn/genome) compared to non-exposed individuals (1.30 ± 0.33mtDNAcn/genome; <i>p</i> = 0.001). The multivariate analysis indicated that individuals who reported using haloxyfop and copper sulfate demonstrated an increase (β = 0.200, <i>p</i> = 0.053) and a decrease (β=-0.2, <i>p</i> = 0.021), respectively, in mtDNAcn. In conclusion, our findings suggest that chronic exposure to pesticides results in changes in mtDNAcn.</p>\",\"PeriodicalId\":14039,\"journal\":{\"name\":\"International Journal of Environmental Health Research\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2024-08-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Journal of Environmental Health Research\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1080/09603123.2023.2280147\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2023/11/15 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"ENVIRONMENTAL SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Environmental Health Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1080/09603123.2023.2280147","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/11/15 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
引用次数: 0
摘要
农药的使用与活性氧产生的增加有关,导致氧化应激,这反过来又会导致基因组不稳定。不稳定性的一个标志是线粒体基因组的拷贝数变异(mtDNAcn),它在包括肿瘤在内的多种人类疾病中被发现发生改变。本研究旨在探讨职业农药暴露个体mtDNAcn的变异。对154名个体(78名暴露者和76名未暴露者)进行了Real-time PCR检测。与未暴露个体(1.30±0.33mtDNAcn/基因组)相比,暴露个体mtDNAcn显著降低(1.11±0.37mtDNAcn/基因组);p = 0.001)。多因素分析表明,使用haloxyfop和硫酸铜的个体mtDNAcn分别升高(β= 0.200, p = 0.053)和降低(β=-0.2, p = 0.021)。总之,我们的研究结果表明,长期暴露于农药会导致mtDNAcn的变化。
Analysis of mitochondrial DNA copy number variation in Brazilian farmers occupationally exposed to pesticides.
The use of pesticide use has been linked to the higher production of reactive oxygen species, resulting in oxidative stress, which in turn can cause genomic instability. A marker for instability is the copy number variation of the mitochondrial genome (mtDNAcn), which has been found to be altered in diverse human diseases, including tumors. This research aimed to examine the variation of mtDNAcn in individuals occupationally exposed to pesticides. Real-time PCR assays were conducted on 154 individuals (78 exposed and 76 non-exposed). Pesticide-exposed ndividuals exhibited a significant reduction in mtDNAcn (1.11 ± 0.37mtDNAcn/genome) compared to non-exposed individuals (1.30 ± 0.33mtDNAcn/genome; p = 0.001). The multivariate analysis indicated that individuals who reported using haloxyfop and copper sulfate demonstrated an increase (β = 0.200, p = 0.053) and a decrease (β=-0.2, p = 0.021), respectively, in mtDNAcn. In conclusion, our findings suggest that chronic exposure to pesticides results in changes in mtDNAcn.
期刊介绍:
International Journal of Environmental Health Research ( IJEHR ) is devoted to the rapid publication of research in environmental health, acting as a link between the diverse research communities and practitioners in environmental health. Published articles encompass original research papers, technical notes and review articles. IJEHR publishes articles on all aspects of the interaction between the environment and human health. This interaction can broadly be divided into three areas: the natural environment and health – health implications and monitoring of air, water and soil pollutants and pollution and health improvements and air, water and soil quality standards; the built environment and health – occupational health and safety, exposure limits, monitoring and control of pollutants in the workplace, and standards of health; and communicable diseases – disease spread, control and prevention, food hygiene and control, and health aspects of rodents and insects. IJEHR is published in association with the International Federation of Environmental Health and includes news from the Federation of international meetings, courses and environmental health issues.