Toxicity Test of Copper (Cu) and Chromium (Cr) on the Growth of Mung Bean (Vigna radiata L.) Sprouts

Industries that are growing rapidly contribute a big role in environmental pollution, one of which is the production of heavy metal waste. One example of heavy metals that are toxic when present in large quantities is Copper (Cu) and Chromium (Cr). The purpose of this experiment is to test the toxicity of the two types of heavy metals Cu and Cr against the seed growth of the Vigna radiata L. plant, find out the type of metal that gives the highest percentage of inhibition to the growth of V. radiata L., and find out the relationship between the two types of heavy metals. The method to determine the level of toxicity and the relationship between the two metals can be known by toxicity tests which include preliminary tests, actual tests and mixed tests on the seeds of V. radiata L. while data analysis is carried out by linear regression and the data is presented in the form of tables and graphs. Preliminary test to determine the ideal range of toxicity concentrations to be continued with the actual test with the addition of Cu and Cr metal toxins to the seeds of V. radiata L. The results of the study on the actual test showed that the IC50 of Cu metal was 127.4 ppm while the IC50 of Cr metal was 615.23 ppm with Cu metal having a more significant negative impact than Cr. In the mixed test, it showed that Cu metal IC50 was 247.5 ppm while Cr metal IC50 was 579.85 ppm with the highest inhibition value at Cu:Cr concentration of 90:10. The toxicant relationship of Cu and Cr based on the additive index is the additive (value S = 0).