{"title":"不同施氮量对玉米耐氮基因ZmNR1基因表达的影响","authors":"Sara I. okab, Ziyad A. Abed","doi":"10.21931/rb/css/2023.08.01.93","DOIUrl":null,"url":null,"abstract":"A field experiment was carried out in the College of Agricultural Engineering Sciences - University of Baghdad, during the fall season of 2021 to find\nout which cultivated cultivars of maize are efficient under nitrogen fertilization.\nThe experiment was applied according to an RCBD (split-plot design with three\nreplications). The cultivars of the experiment (Baghdad, 5018, Sarah) supply\nthree levels of nitrogen fertilizer, which are N1 (100 kg.N/ha), N2 (200 kg.N/ha)\nand N3 (300 kg.N/ha). The statistical analysis results showed the superiority of\nthe Sarah genotype, which gave the highest value of SOD and CAT enzymes,\nreaching 11.59 units mg-1 and 10.76 units mg-1\n. Protein sequentially, while cultivar5018 outperformed as it gave the highest value of POD enzyme, which was\n5.43 units mg-1.protein, and there were no significant differences between genotypes in NR enzyme. The increase in nitrogen fertilizer caused an increase in the\nvalues of oxidation and reduction enzymes, as the nitrogen level N3 gave the\nhighest value for SOD, POD, CAT and nitrate reduction enzymes NR, reaching\n11.59 mg-1\nand 6.94 mg-1\nunits. Protein and 16.40 mg-1 units. Protein and 6.30\nmg-1 units. Protein sequentially. The results of the molecular analysis using the\nReal-Time PCR technique showed the expression of the ZmNR1 gene. The analysis showed that the cultivated genotypes contained the gene in varying proportions as the gene expression increased in the compositions to which the nitrogen\nfertilizer was added. The value ranged from (0.16) to (49.46) times (a copy of the\ngene), where the highest expression of the gene was (49.46) for the Sarah cultivar\nwhen The nitrogen level N2 also gave the same gene expression ZmNR1 (15.01)\nfolds. The cultivars of maize varied among them in their tolerance to excess or\ndeficiency of nitrogen and in their ability to express the ZmNR1 gene, one of the\nmost important nitrogen-carrying genes for maize crops.\nKeywords: maize, gene expression, nitrate reductase, antioxidant enzymes","PeriodicalId":443152,"journal":{"name":"Sumer 1","volume":"37 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2023-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Gene expression of a nitrogen tolerance gene ZmNR1 under the influence of different levels of nitrogen in maize\",\"authors\":\"Sara I. okab, Ziyad A. Abed\",\"doi\":\"10.21931/rb/css/2023.08.01.93\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A field experiment was carried out in the College of Agricultural Engineering Sciences - University of Baghdad, during the fall season of 2021 to find\\nout which cultivated cultivars of maize are efficient under nitrogen fertilization.\\nThe experiment was applied according to an RCBD (split-plot design with three\\nreplications). The cultivars of the experiment (Baghdad, 5018, Sarah) supply\\nthree levels of nitrogen fertilizer, which are N1 (100 kg.N/ha), N2 (200 kg.N/ha)\\nand N3 (300 kg.N/ha). The statistical analysis results showed the superiority of\\nthe Sarah genotype, which gave the highest value of SOD and CAT enzymes,\\nreaching 11.59 units mg-1 and 10.76 units mg-1\\n. Protein sequentially, while cultivar5018 outperformed as it gave the highest value of POD enzyme, which was\\n5.43 units mg-1.protein, and there were no significant differences between genotypes in NR enzyme. The increase in nitrogen fertilizer caused an increase in the\\nvalues of oxidation and reduction enzymes, as the nitrogen level N3 gave the\\nhighest value for SOD, POD, CAT and nitrate reduction enzymes NR, reaching\\n11.59 mg-1\\nand 6.94 mg-1\\nunits. Protein and 16.40 mg-1 units. Protein and 6.30\\nmg-1 units. Protein sequentially. The results of the molecular analysis using the\\nReal-Time PCR technique showed the expression of the ZmNR1 gene. The analysis showed that the cultivated genotypes contained the gene in varying proportions as the gene expression increased in the compositions to which the nitrogen\\nfertilizer was added. The value ranged from (0.16) to (49.46) times (a copy of the\\ngene), where the highest expression of the gene was (49.46) for the Sarah cultivar\\nwhen The nitrogen level N2 also gave the same gene expression ZmNR1 (15.01)\\nfolds. The cultivars of maize varied among them in their tolerance to excess or\\ndeficiency of nitrogen and in their ability to express the ZmNR1 gene, one of the\\nmost important nitrogen-carrying genes for maize crops.\\nKeywords: maize, gene expression, nitrate reductase, antioxidant enzymes\",\"PeriodicalId\":443152,\"journal\":{\"name\":\"Sumer 1\",\"volume\":\"37 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2023-08-15\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Sumer 1\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.21931/rb/css/2023.08.01.93\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Sumer 1","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.21931/rb/css/2023.08.01.93","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
研究人员于2021年秋季在巴格达大学农业工程科学学院进行了一项田间试验,以确定哪些栽培玉米品种在氮肥施用下效率较高。试验采用RCBD (split-plot design with three duplicate)设计。试验品种(巴格达、5018、萨拉)施氮水平分别为N1 (100 kg.N/ha)、N2 (200 kg.N/ha)和N3 (300 kg.N/ha)。统计分析结果显示Sarah基因型的优势,其SOD和CAT酶最高,分别达到11.59单位mg-1和10.76单位mg-1。而cultivar5018的POD酶含量最高,为5.43单位mg-1。NR酶基因型间差异不显著。氮肥用量的增加导致氧化还原酶值的增加,其中氮肥水平N3使SOD、POD、CAT和硝酸盐还原酶NR值最高,分别达到11.59 mg-1和6.94 mg-1units。蛋白质和16.40毫克-1单位。蛋白质和6.30毫克-1单位。蛋白质的顺序。real - time PCR技术分子分析结果显示ZmNR1基因表达。分析表明,随着氮肥组合物中该基因表达量的增加,培养的基因型中含有该基因的比例也不同。该基因的表达量为(0.16)~(49.46)倍(1个拷贝),其中氮水平N2也使该基因的表达量达到最高(49.46)倍(15.01)倍。不同玉米品种对氮素过剩或缺乏的耐受性以及表达ZmNR1基因的能力存在差异,ZmNR1基因是玉米作物最重要的携氮基因之一。关键词:玉米,基因表达,硝酸还原酶,抗氧化酶
Gene expression of a nitrogen tolerance gene ZmNR1 under the influence of different levels of nitrogen in maize
A field experiment was carried out in the College of Agricultural Engineering Sciences - University of Baghdad, during the fall season of 2021 to find
out which cultivated cultivars of maize are efficient under nitrogen fertilization.
The experiment was applied according to an RCBD (split-plot design with three
replications). The cultivars of the experiment (Baghdad, 5018, Sarah) supply
three levels of nitrogen fertilizer, which are N1 (100 kg.N/ha), N2 (200 kg.N/ha)
and N3 (300 kg.N/ha). The statistical analysis results showed the superiority of
the Sarah genotype, which gave the highest value of SOD and CAT enzymes,
reaching 11.59 units mg-1 and 10.76 units mg-1
. Protein sequentially, while cultivar5018 outperformed as it gave the highest value of POD enzyme, which was
5.43 units mg-1.protein, and there were no significant differences between genotypes in NR enzyme. The increase in nitrogen fertilizer caused an increase in the
values of oxidation and reduction enzymes, as the nitrogen level N3 gave the
highest value for SOD, POD, CAT and nitrate reduction enzymes NR, reaching
11.59 mg-1
and 6.94 mg-1
units. Protein and 16.40 mg-1 units. Protein and 6.30
mg-1 units. Protein sequentially. The results of the molecular analysis using the
Real-Time PCR technique showed the expression of the ZmNR1 gene. The analysis showed that the cultivated genotypes contained the gene in varying proportions as the gene expression increased in the compositions to which the nitrogen
fertilizer was added. The value ranged from (0.16) to (49.46) times (a copy of the
gene), where the highest expression of the gene was (49.46) for the Sarah cultivar
when The nitrogen level N2 also gave the same gene expression ZmNR1 (15.01)
folds. The cultivars of maize varied among them in their tolerance to excess or
deficiency of nitrogen and in their ability to express the ZmNR1 gene, one of the
most important nitrogen-carrying genes for maize crops.
Keywords: maize, gene expression, nitrate reductase, antioxidant enzymes