D. Angeli, I. C. M. Andrei, A. Dekker, Claudio Takeo Ueno, L. F. Dias
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Estudo da hidrólise ácida e enzimática da borra de café como alternativa de uso deste resíduo
The soluble coffee industry produces a large amount of coffee grounds waste with many applications. The aim of this study was to assess the effectiveness of acid hydrolysis (HCl) and enzymatic (Viscozyme L) of coffee grounds waste. The acid hydrolysis was performed in different conditions of temperature and pressure using water bath and autoclave. The optimization of the parameters of enzymatic hydrolysis (pH, time, temperature) were determined by central composite design (CCRD) using as enzyme substrate, Whatman no1 paper. The best conditions obtained for hydrolysis of cellulose filter paper were pH 4.0 at 45 °C / hour, equivalent to 50 FBG (β-glucanase fungal) but the coffee grounds enzymatically hydrolyzed under these conditions, did not provide satisfactory results. Thus, it was decided by a combination of enzymatic hydrolysis followed by acid hydrolysis using hydrochloric acid (120 °C / 2 h) to yield a significant amount of glucose compared to acid hydrolysis. PALAVRAS-CHAVE: Resíduo lignocelulósico, Viscozyme L, Planejamento experimental.
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