寻找内啡肽-1,4-β-葡聚糖酶活性生产者用于植物残体的生物破坏

Chabaniuk Ya. V., Brovko I. S., Melnikova I. O., Spataru K. V.
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Hole method based on the interaction between Congo red dye and polysaccharide containing β (1.4) or β (1.3) bonds (mannitol-yeast medium was applied for deep cultivation of B. subtilis and P. polymyxa, corn-molasses — for C. globosum and T. harzianum), and spectrophotometric method based on colorimetric determination of the optical density of ferricyanide solution, the excess of which remains after reaction with reducing substances present in the culture fluid (microorganisms were cultured on corn-molasses medium). Results. Both hole and spectrophotometric methods showed that the studied micromycete strains had higher endo-1,4-β-glucanase activity than bacterial strains. The activity of endo-1,4-β-glucanase of microorganisms is as follows: B. subtilis eko/206 — 0.0499 IU/ml, T. harzianum eko/101 — 0.0667 IU/ml; C. globosum eko/108 — 0.0673 IU/ml. 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引用次数: 0

摘要

目标。评估土壤微生物枯草芽孢杆菌、多粘芽孢杆菌、球毛菌和哈茨木霉的内切-1,4-β-葡聚糖酶的活性,探讨它们作为生物技术生产中的酶源和创建植物残留物生物破坏者的潜力。方法。采用孔洞法,利用刚果红染料与含有β(1.4)或β(1.3)键的多糖(甘露醇-酵母培养基)相互作用,对枯草芽孢杆菌(B. subtilis)和多粘菌(P. polymyxa)、玉米-糖蜜-进行深度培养对于C. globosum和T. harzianum),以及基于比色法测定铁氰化物溶液光密度的分光光度法,过量的铁氰化物溶液与培养液中存在的还原物质反应后残留(微生物在玉米糖蜜培养基上培养)。结果。空穴法和分光光度法均表明,所研究的微菌菌株具有较高的内切-1,4-β-葡聚糖酶活性。微生物endo-1,4-β-葡聚糖酶活性分别为:枯草芽孢杆菌eko/206 ~ 0.0499 IU/ml,哈氏芽孢杆菌eko/101 ~ 0.0667 IU/ml;C. globosum eko/108 - 0.0673 IU/ml。启迪带的平均直径为:哈兹兰(T. harzianum) /101 ~ 27.00 mm;C. globosum eko/108 - 28.14 mm;枯草芽孢杆菌eko/206 - 20.25毫米。多粘菌eko/204未检测到内切葡聚糖酶活性。结论。结果表明,C. globosum eko/108和T. harzianum eko/101菌株的酶活性最高,表明利用这些菌株进行生物技术制备endo-1,4-β-葡聚糖酶具有良好的应用前景。虽然枯草芽孢杆菌eko/206具有产生纤维素水解酶的能力,但其数量相对较少,因此将其用作内切-1,4-β-葡聚糖酶的生产者不太合适。P. polymyxa eko/204不显示内切葡聚糖酶活性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
SEARCHING ENDO-1,4-β-GLUCANASE ACTIVE PRODUCERS FOR BIODESTRUCTION OF PLANT RESIDUES
Objective. Evaluate the activity of endo-1,4-β-glucanase in soil microorganisms Bacillus subtilis, Paenibacillus polymyxa, Chaetomium globosum and Trichoderma harzianum for their potential use as an enzyme source in biotechnological production and to create a biodestroyer of plant residues. Methods. Hole method based on the interaction between Congo red dye and polysaccharide containing β (1.4) or β (1.3) bonds (mannitol-yeast medium was applied for deep cultivation of B. subtilis and P. polymyxa, corn-molasses — for C. globosum and T. harzianum), and spectrophotometric method based on colorimetric determination of the optical density of ferricyanide solution, the excess of which remains after reaction with reducing substances present in the culture fluid (microorganisms were cultured on corn-molasses medium). Results. Both hole and spectrophotometric methods showed that the studied micromycete strains had higher endo-1,4-β-glucanase activity than bacterial strains. The activity of endo-1,4-β-glucanase of microorganisms is as follows: B. subtilis eko/206 — 0.0499 IU/ml, T. harzianum eko/101 — 0.0667 IU/ml; C. globosum eko/108 — 0.0673 IU/ml. The average diameters of the enlightenment zones are as follows: T. harzianum eko/101 — 27.00 mm; C. globosum eko/108 — 28.14 mm; B. subtilis eko/206 — 20.25 mm. No endoglucanase activity was detected in P. polymyxa eko/204. Conclusion. The study of endo-1,4-β- glucanase activity in strains of microorganisms showed that the highest enzymatic activity is observed in C. globosum eko/108 and T. harzianum eko/101, suggesting the prospects of using these strains to obtain endo-1,4-β-glucanase via biotechnology. Although B. subtilis eko/206 has the ability to produce cellulolytic enzymes but their number is relatively small, so its use as a producer of endo-1,4-β-glucanase is less appropriate. P. polymyxa eko/204 did not show endoglucanase activity.
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