牙周健康与疾病患者牙龈卟啉单胞菌计数及唾液抗牙龈卟啉单胞菌脂多糖免疫球蛋白A的比较

Abhinav S Baheti, P. Pudakalkatti, K. Bhat
{"title":"牙周健康与疾病患者牙龈卟啉单胞菌计数及唾液抗牙龈卟啉单胞菌脂多糖免疫球蛋白A的比较","authors":"Abhinav S Baheti, P. Pudakalkatti, K. Bhat","doi":"10.4103/dmr.dmr_9_21","DOIUrl":null,"url":null,"abstract":"Background: Porphyromonas gingivalis (P. gingivalis) have been implicated to play a role in the pathogenesis of periodontal disease. Aim: The aim of this study was to compare P. gingivalis in subgingival plaque samples and salivary (immunoglobulin A [IgA]) antibodies against lipopolysaccharide (LPS) of P. gingivalis in periodontal health and disease. Materials and Methods: Totally sixty subjects were included, thirty subjects in healthy group and thirty subjects in chronic periodontitis group. Subgingival plaque and unstimulated saliva samples were obtained from each study subject. P. gingivalis (colony-forming units [CFUs]) were detected using culture method, and salivary IgA levels were assessed using enzyme-linked immunosorbent assay technique. Comparisons between the healthy and chronic periodontitis groups were done for P. gingivalis CFUs/ml (CFUs/milliliter) as well as for salivary IgA levels using Mann–Whitney U-test. Correlation between CFUs/ml of P. gingivalis and salivary IgA levels was also assessed in both the groups. Results: Although P. gingivalis CFUs/ml count was more in the chronic periodontitis group, difference was not statistically significant. Salivary IgA levels were significantly higher in the chronic periodontitis group compared to the healthy group. No correlation was observed between CFUs/ml of P. gingivalis and salivary IgA levels in the healthy group as well as the chronic periodontitis group. Conclusions: Increased levels of specific salivary antibodies (IgA) against LPS from P. gingivalis are associated with periodontal disease. Salivary IgA level against LPS from P. gingivalis can be a promising indicator in the serological diagnosis of periodontal disease.","PeriodicalId":413497,"journal":{"name":"Dentistry and Medical Research","volume":"19 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Comparison of Porphyromonas gingivalis count and salivary immunoglobulin A against lipopolysaccharide of Porphyromonas gingivalis in periodontal health and disease\",\"authors\":\"Abhinav S Baheti, P. Pudakalkatti, K. Bhat\",\"doi\":\"10.4103/dmr.dmr_9_21\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Background: Porphyromonas gingivalis (P. gingivalis) have been implicated to play a role in the pathogenesis of periodontal disease. Aim: The aim of this study was to compare P. gingivalis in subgingival plaque samples and salivary (immunoglobulin A [IgA]) antibodies against lipopolysaccharide (LPS) of P. gingivalis in periodontal health and disease. Materials and Methods: Totally sixty subjects were included, thirty subjects in healthy group and thirty subjects in chronic periodontitis group. Subgingival plaque and unstimulated saliva samples were obtained from each study subject. P. gingivalis (colony-forming units [CFUs]) were detected using culture method, and salivary IgA levels were assessed using enzyme-linked immunosorbent assay technique. Comparisons between the healthy and chronic periodontitis groups were done for P. gingivalis CFUs/ml (CFUs/milliliter) as well as for salivary IgA levels using Mann–Whitney U-test. Correlation between CFUs/ml of P. gingivalis and salivary IgA levels was also assessed in both the groups. Results: Although P. gingivalis CFUs/ml count was more in the chronic periodontitis group, difference was not statistically significant. Salivary IgA levels were significantly higher in the chronic periodontitis group compared to the healthy group. No correlation was observed between CFUs/ml of P. gingivalis and salivary IgA levels in the healthy group as well as the chronic periodontitis group. Conclusions: Increased levels of specific salivary antibodies (IgA) against LPS from P. gingivalis are associated with periodontal disease. Salivary IgA level against LPS from P. gingivalis can be a promising indicator in the serological diagnosis of periodontal disease.\",\"PeriodicalId\":413497,\"journal\":{\"name\":\"Dentistry and Medical Research\",\"volume\":\"19 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Dentistry and Medical Research\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4103/dmr.dmr_9_21\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Dentistry and Medical Research","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4103/dmr.dmr_9_21","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

背景:牙龈卟啉单胞菌(P. gingivalis)已被认为在牙周病的发病机制中发挥作用。目的:本研究的目的是比较牙龈下菌斑样本中的牙龈假单胞菌和唾液中抗牙龈假单胞菌脂多糖(LPS)的抗体(免疫球蛋白A [IgA])在牙周健康和疾病中的作用。材料与方法:共60例受试者,健康组30例,慢性牙周炎组30例。每位研究对象的牙龈下菌斑和未受刺激的唾液样本均被采集。采用培养法检测牙龈卟啉单胞菌(菌落形成单位[cfu]),采用酶联免疫吸附法检测唾液IgA水平。比较健康组和慢性牙周炎组牙龈P. CFUs/ml (CFUs/毫升)和唾液IgA水平,使用Mann-Whitney u检验。还评估了两组牙龈假单胞菌CFUs/ml与唾液IgA水平的相关性。结果:虽然慢性牙周炎组牙龈假单胞菌cfu /ml计数较多,但差异无统计学意义。慢性牙周炎组的唾液IgA水平明显高于健康组。健康组和慢性牙周炎组牙龈假单胞菌CFUs/ml与唾液IgA水平无相关性。结论:牙龈卟啉单胞菌的特异性唾液抗体(IgA)水平升高与牙周病有关。唾液中抗牙龈卟啉单胞菌LPS的IgA水平可作为牙周病血清学诊断的重要指标。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparison of Porphyromonas gingivalis count and salivary immunoglobulin A against lipopolysaccharide of Porphyromonas gingivalis in periodontal health and disease
Background: Porphyromonas gingivalis (P. gingivalis) have been implicated to play a role in the pathogenesis of periodontal disease. Aim: The aim of this study was to compare P. gingivalis in subgingival plaque samples and salivary (immunoglobulin A [IgA]) antibodies against lipopolysaccharide (LPS) of P. gingivalis in periodontal health and disease. Materials and Methods: Totally sixty subjects were included, thirty subjects in healthy group and thirty subjects in chronic periodontitis group. Subgingival plaque and unstimulated saliva samples were obtained from each study subject. P. gingivalis (colony-forming units [CFUs]) were detected using culture method, and salivary IgA levels were assessed using enzyme-linked immunosorbent assay technique. Comparisons between the healthy and chronic periodontitis groups were done for P. gingivalis CFUs/ml (CFUs/milliliter) as well as for salivary IgA levels using Mann–Whitney U-test. Correlation between CFUs/ml of P. gingivalis and salivary IgA levels was also assessed in both the groups. Results: Although P. gingivalis CFUs/ml count was more in the chronic periodontitis group, difference was not statistically significant. Salivary IgA levels were significantly higher in the chronic periodontitis group compared to the healthy group. No correlation was observed between CFUs/ml of P. gingivalis and salivary IgA levels in the healthy group as well as the chronic periodontitis group. Conclusions: Increased levels of specific salivary antibodies (IgA) against LPS from P. gingivalis are associated with periodontal disease. Salivary IgA level against LPS from P. gingivalis can be a promising indicator in the serological diagnosis of periodontal disease.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信