D. Jiang, Shi-ying Zheng, Jun Zhao, J. Ge, Hong Li
{"title":"热疗对A549和H1299肺癌细胞生长影响的研究","authors":"D. Jiang, Shi-ying Zheng, Jun Zhao, J. Ge, Hong Li","doi":"10.1109/FBIE.2009.5405886","DOIUrl":null,"url":null,"abstract":"To observe the influence of hyperther- mia under different temperature on the growth, apoptosis of Human Lung Cancer cell of A549, H1299 to investi- gate the possible mechanism of apoptosis in lung cancer cell caused by p53 with different activity. Human Lung Cancer cell of A549, H1299 was heated under 40°C 42°C 44°C separately for 2 hours each in the water bath case. After the hyperthermia under different temperature, the inhibition ability of A549 cells and H1299 cells were determined by MTT, the apoptosis and cell cycle of A549 cells and H1299 cells were analyzed by FACS, and RT-PCR was adopted to detect the expression of the apoptosis related gene of p53 and caspase-3.","PeriodicalId":333255,"journal":{"name":"2009 International Conference on Future BioMedical Information Engineering (FBIE)","volume":"838 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2009-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"The research on the effect of hyperthermia on growth of A549 and H1299 lung cancer cells\",\"authors\":\"D. Jiang, Shi-ying Zheng, Jun Zhao, J. Ge, Hong Li\",\"doi\":\"10.1109/FBIE.2009.5405886\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"To observe the influence of hyperther- mia under different temperature on the growth, apoptosis of Human Lung Cancer cell of A549, H1299 to investi- gate the possible mechanism of apoptosis in lung cancer cell caused by p53 with different activity. Human Lung Cancer cell of A549, H1299 was heated under 40°C 42°C 44°C separately for 2 hours each in the water bath case. After the hyperthermia under different temperature, the inhibition ability of A549 cells and H1299 cells were determined by MTT, the apoptosis and cell cycle of A549 cells and H1299 cells were analyzed by FACS, and RT-PCR was adopted to detect the expression of the apoptosis related gene of p53 and caspase-3.\",\"PeriodicalId\":333255,\"journal\":{\"name\":\"2009 International Conference on Future BioMedical Information Engineering (FBIE)\",\"volume\":\"838 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2009-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"2009 International Conference on Future BioMedical Information Engineering (FBIE)\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1109/FBIE.2009.5405886\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"2009 International Conference on Future BioMedical Information Engineering (FBIE)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/FBIE.2009.5405886","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
The research on the effect of hyperthermia on growth of A549 and H1299 lung cancer cells
To observe the influence of hyperther- mia under different temperature on the growth, apoptosis of Human Lung Cancer cell of A549, H1299 to investi- gate the possible mechanism of apoptosis in lung cancer cell caused by p53 with different activity. Human Lung Cancer cell of A549, H1299 was heated under 40°C 42°C 44°C separately for 2 hours each in the water bath case. After the hyperthermia under different temperature, the inhibition ability of A549 cells and H1299 cells were determined by MTT, the apoptosis and cell cycle of A549 cells and H1299 cells were analyzed by FACS, and RT-PCR was adopted to detect the expression of the apoptosis related gene of p53 and caspase-3.