热疗对A549和H1299肺癌细胞生长影响的研究

D. Jiang, Shi-ying Zheng, Jun Zhao, J. Ge, Hong Li
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引用次数: 1

摘要

观察不同温度下高温对人肺癌细胞A549、H1299生长和凋亡的影响,探讨不同活性p53诱导肺癌细胞凋亡的可能机制。将人肺癌细胞A549、H1299分别在40℃、42℃、44℃条件下分别水浴加热2小时。不同温度热疗后,MTT检测A549细胞和H1299细胞的抑制能力,FACS分析A549细胞和H1299细胞的凋亡和细胞周期,RT-PCR检测凋亡相关基因p53和caspase-3的表达。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The research on the effect of hyperthermia on growth of A549 and H1299 lung cancer cells
To observe the influence of hyperther- mia under different temperature on the growth, apoptosis of Human Lung Cancer cell of A549, H1299 to investi- gate the possible mechanism of apoptosis in lung cancer cell caused by p53 with different activity. Human Lung Cancer cell of A549, H1299 was heated under 40°C 42°C 44°C separately for 2 hours each in the water bath case. After the hyperthermia under different temperature, the inhibition ability of A549 cells and H1299 cells were determined by MTT, the apoptosis and cell cycle of A549 cells and H1299 cells were analyzed by FACS, and RT-PCR was adopted to detect the expression of the apoptosis related gene of p53 and caspase-3.
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