{"title":"红树根内生细菌纤维素降解菌株的筛选","authors":"Silvera Devy","doi":"10.37859/jp.v12i2.3371","DOIUrl":null,"url":null,"abstract":"Cellulose agricultural waste is a source of environmental pollution and an alternative solution to overcome it is to degrade this waste with the help of cellulolytic enzymes. The Biochemistry Laboratory of FMIPA Riau University has a number of endophytic bacterial isolates from the roots of Mangrove Ceriops tagal (Perr) C. B. Rob in the Bengkalis area whose potential to produce cellulolytic enzymes is not yet known. Qualitative selection in carboxy methyl cellulose (CMC) solid media from 24 isolates of endophytic bacteria that were able to live because they produced cellulolytic enzymes, only 22 isolates. Quantitatively using the DNS (Dinitro Salicylic Acid) method, 3 of these 22 isolates had relatively higher activity than other isolates. The activity of isolate C-9 (LBKURCC332) was 572x10-4±0.0069U/mL, isolate C-23(LBKURCC346) was 423x10-4±0.0027U/mL and isolate C-30 (LBKURCC353) was 384x10-4± 0.0003 U/mL and statistically these 3 cellulolytic activities were significantly different","PeriodicalId":219962,"journal":{"name":"Photon: Jurnal Sain dan Kesehatan","volume":"1 1","pages":"0"},"PeriodicalIF":0.0000,"publicationDate":"2022-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"SELECTION OF ISOLATES OF LOCAL ENDOPHYTIC BACTERIA CELLULOLYTIC STRAINS FROM MANGROVE ROOTS Ceriops tagal (Perr) C. B. Rob\",\"authors\":\"Silvera Devy\",\"doi\":\"10.37859/jp.v12i2.3371\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Cellulose agricultural waste is a source of environmental pollution and an alternative solution to overcome it is to degrade this waste with the help of cellulolytic enzymes. The Biochemistry Laboratory of FMIPA Riau University has a number of endophytic bacterial isolates from the roots of Mangrove Ceriops tagal (Perr) C. B. Rob in the Bengkalis area whose potential to produce cellulolytic enzymes is not yet known. Qualitative selection in carboxy methyl cellulose (CMC) solid media from 24 isolates of endophytic bacteria that were able to live because they produced cellulolytic enzymes, only 22 isolates. Quantitatively using the DNS (Dinitro Salicylic Acid) method, 3 of these 22 isolates had relatively higher activity than other isolates. The activity of isolate C-9 (LBKURCC332) was 572x10-4±0.0069U/mL, isolate C-23(LBKURCC346) was 423x10-4±0.0027U/mL and isolate C-30 (LBKURCC353) was 384x10-4± 0.0003 U/mL and statistically these 3 cellulolytic activities were significantly different\",\"PeriodicalId\":219962,\"journal\":{\"name\":\"Photon: Jurnal Sain dan Kesehatan\",\"volume\":\"1 1\",\"pages\":\"0\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-07-14\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Photon: Jurnal Sain dan Kesehatan\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.37859/jp.v12i2.3371\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Photon: Jurnal Sain dan Kesehatan","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.37859/jp.v12i2.3371","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
纤维素农业废物是环境污染的一个来源，克服它的另一种解决方案是在纤维素水解酶的帮助下降解这些废物。廖内大学生物化学实验室从Bengkalis地区的红树林Ceriops tagal (Perr) C. B. Rob的根部分离出了一些内生细菌，这些细菌产生纤维素水解酶的潜力尚不清楚。在羧甲基纤维素(CMC)固体培养基中定性筛选出24株能产生纤维素水解酶而存活的内生细菌，仅有22株。采用二硝基水杨酸(Dinitro Salicylic Acid, DNS)法定量分析，22株分离株中有3株活性较高。分离物C-9 (LBKURCC332)的活性为572x10-4±0.0069U/mL，分离物C-23(LBKURCC346)的活性为423x10-4±0.0027U/mL，分离物C-30 (LBKURCC353)的活性为384x10-4±0.0003 U/mL，具有统计学差异
SELECTION OF ISOLATES OF LOCAL ENDOPHYTIC BACTERIA CELLULOLYTIC STRAINS FROM MANGROVE ROOTS Ceriops tagal (Perr) C. B. Rob
Cellulose agricultural waste is a source of environmental pollution and an alternative solution to overcome it is to degrade this waste with the help of cellulolytic enzymes. The Biochemistry Laboratory of FMIPA Riau University has a number of endophytic bacterial isolates from the roots of Mangrove Ceriops tagal (Perr) C. B. Rob in the Bengkalis area whose potential to produce cellulolytic enzymes is not yet known. Qualitative selection in carboxy methyl cellulose (CMC) solid media from 24 isolates of endophytic bacteria that were able to live because they produced cellulolytic enzymes, only 22 isolates. Quantitatively using the DNS (Dinitro Salicylic Acid) method, 3 of these 22 isolates had relatively higher activity than other isolates. The activity of isolate C-9 (LBKURCC332) was 572x10-4±0.0069U/mL, isolate C-23(LBKURCC346) was 423x10-4±0.0027U/mL and isolate C-30 (LBKURCC353) was 384x10-4± 0.0003 U/mL and statistically these 3 cellulolytic activities were significantly different
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