OPTIMIZATION OF THE PROCEDURE OF LACTATE AND PYRUVATE DETERMINATION IN BLOOD SERUM USING A BIOSENSOR SYSTEM

The purpose of this work was to optimize the procedure for simultaneous determination of concentrations of lactate and pyruvate in human serum using a biosensor system. The system consisted of two amperometric biosensors based on pyruvate oxidase and lactate oxidase, respectively, that were connected to one measuring circuit and simultaneously operated in a single measuring cell. In the work, the dependence of the responses of the biosensor system on the dilution of serum in the working cell was tested. The reproducibility of the responses and the change in the calibration curves of the biosensor system when working with serum were studied. The concentrations of lactate, pyruvate and their ratio in blood serum samples were determined and a high correlation of the results with the data of the control method was shown. It is proved that the proposed biosensor system is suitable for the rapid determination of lactate, pyruvate and their ratio and can be used in the medical diagnostics of the relevant diseases.