无花果叶、叶柄和果实提取物中生物活性物质的筛选

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引用次数: 0

摘要

对无花果水提取物、乙醇提取物和无花果乳进行了初步的植物化学筛选和抗致病菌试验。定性分析结果显示,单宁、皂苷、黄酮类、萜类、酞菁类和甾体类均为阳性。定量分析表明,无花果乳中生物碱含量最高,为41%,植物叶片中生物碱含量最高,为39%。此外,用圆盘扩散法测定了无花果奶的粗提取物和植物提取物的抑菌活性。水提物和乙醇提物对一些致病性革兰氏阳性菌金黄色葡萄球菌和肺炎链球菌的影响铜绿假单胞菌、肺炎克雷伯菌、大肠埃希菌、普通变形杆菌、SPP沙门氏菌和产气克雷伯菌两种革兰氏阴性菌。通过测定抑菌带直径,比较微生物对植物提取物的敏感性,并与指定的抗生素进行比较,孵育后以mm为单位测定抑菌带,抑菌效果较好,抑菌范围大于6 mm,表明植物叶片提取物和无花果奶中的生物活性化合物均具有有效的抑菌活性。此外,还讨论了植物叶提取物和无花果乳的抑菌活性,它们的植物化学成分对6种被试菌株都有良好的抑菌带。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Screening for Bioactive Compounds in Extracts from Leaves, Leafstalks, and Fruits of Ficus Carica
Aqueous, Ethanol extracts and Fig's milk were preliminary phytochemically screened and tested against some pathogenic bacteria. Qualitatively analysis revealed Tannin, Saponin, Flavonoids and Terpenoids and phlobatannins and Steroids gave positive results. Quantitative analysis showed that the highest percentage of alkaloid was 41% in fig milk and 39% in plant leaves. In addition, the crude extracts of fig's milk as well as of the raw plant extracts were tested (using the Disc Diffusion Method) for their antimicrobial activity against the bacterial pathogens. The influences of aqueous and ethanol extracts on some pathogenic: two strains of gram-positive bacteria include Staphylococcus aureus and Streptococcus pneumonia.., and two strains of gram-negative bacteria including Pseudomonas aeruginosa, Klebsiella pneumoniae, Escherichia coli, Proteus vulgaris, Salmonella SPP and Klebsiella aerogenes. , and the sensitivity of the microorganisms to the extracts of plant’s species were compared with each other and with designated antibiotics by measuring the diameter of the inhibition zones, After incubation, the zone of inhibition was measured in mm, a good inhibition of more than 6 mm were observed indicating the effective antibacterial activity of the bioactive compounds in both of the plants leaves extracts and fig's milk. Moreover, the including results of the antibacterial activities of plants leaves extracts and fig's milk were discussed regarding their phytochemical components which exhibited that have a good inhibition zone against six tested bacterial strains.
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