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引用次数: 0
摘要
为了检验大鼠GOX-1 (HAO-1)和XDH-1多态性是由一个位点决定还是由两个不同的紧密相连的位点决定，我们进行了生化和遗传学研究。除了电泳上相似的迁移行为外，这两种多态性的表型具有主要的生化特性，例如对50和70℃和等电点热失活的响应。这表明它们只代表一种酶。对50个回交群体[(SPRD-Cu3 × BN)F1 × SPRD Cu3]和14个LXB重组自交系(祖菌株LEW和BN)的后代进行的连锁分析支持了单酶假设，其中没有观察到单一的重组事件。后者的发现与在93个自交系和亚系中所调查的GOX-1/XDH-1分布模式非常吻合(BENDER et al. 1984)。由于这些结果以及与乙醇酸的优先反应性，我们建议将这种多态性仅命名为乙醇酸氧化酶(GOX-1)。
Comparative biochemical and genetic studies of the GOX-1 (HAO-1) and the XDH-1 polymorphism in the rat (Rattus norvegicus).
Biochemical and genetic studies have been carried out to test whether the GOX-1 (HAO-1) and the XDH-1 polymorphism in the rat are determined by one locus or by two different closely linked loci. Besides similar migration behaviour upon electrophoresis the phenotypes of both polymorphisms share major biochemical properties such as responses to heat inactivation at 50 and 70 degrees C and isoelectric points. This suggests that they represent only one enzyme. The one-enzyme hypothesis is supported by linkage analyses performed in 50 offspring of a backcross population [(SPRD-Cu3 x BN)F1 x SPRD Cu3] and 14 LXB recombinant inbred strains (progenitor strains LEW and BN) in which not a single recombinational event can be observed. The latter finding agrees well with the GOX-1/XDH-1 distribution pattern investigated in 93 inbred strains and substrains (BENDER et al. 1984). Because of these results and due to a preferential reactivity with glycolic acid we propose to designate this polymorphism only as glycolate oxidase (GOX-1).
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