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Jurnal Ilmu Nutrisi dan Teknologi Pakan Pub Date : 2021-08-31 DOI:10.29244/jintp.19.2.59-65

D. Harianja, M. H. Karti, I. Prihantoro, Jurnal Ilmu Nutrisi, dan Teknologi Pakan

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引用次数: 3

摘要

苜蓿在干燥胁迫条件下不能达到最佳生长状态。在许多栽培系统中，干旱抑制了苜蓿的生长，减少了苜蓿的产量。伽玛射线辐照育种和聚乙二醇体外选择发现了适应干旱条件的体细胞无性系变异。本研究旨在确定在干燥胁迫条件下具有最佳形态的突变类型。采用体外传代培养法对辐照后的紫花苜蓿突变体进行培养。在PEG培养基中培养的苜蓿突变体，根据处理。本实验采用完全随机因子设计，有2个因素，即γ射线辐照剂量(0 Gy、100 Gy、200 Gy、300 Gy、400 Gy和500 Gy)和PEG浓度(5%、10%、15%和20%)。观察到的变量包括活力、株高、叶片数、湿重、中等收缩、叶片萎蔫和叶片颜色。结果表明，在干胁迫条件下，γ辐照突变体的形态优于对照(0 Gy) (p<0.05)。500 Gy和20% PEG浓度组合产生的突变体类型最佳。关键词:紫花苜蓿，离体，伽马射线，聚乙二醇

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Morfologi Mutan Alfalfa (Medicago sativa L.) Hasil Iradiasi Sinar Gamma Pada Cekaman Kering

Alfalfa in dry stress conditions cannot grow optimally. Drought inhibits growth and reduces alfalfa production in many cultivation systems. Plants breeding with gamma-ray irradiation and in vitro selection using PEG found somaclonal variants adapted to grow well in drought conditions. This study aims to determine the type of mutation with the best morphology in dry stress conditions. Alfalfa mutants resulting from gamma irradiation were sub-cultured in vitro. Alfalfa mutants cultured in PEG medium, according to treatments. This experiment used completely randomized factorial design with 2 factors, namely the gamma-ray irradiation doses (0 Gy, 100 Gy, 200 Gy, 300 Gy, 400 and 500 Gy), and PEG concentrations (5%, 10%, 15%, and 20% ). The variables observed were viability, plant height, number of leaves, wet weight, medium shrinkage, leaf wilting, and leaf color. The results showed that the gamma-irradiated mutant had a better (p<0.05) morphology than the control (0 Gy) in the face of dry stress conditions. Combination of 500 Gy and 20% PEG concentration resulted the best type of mutant. Key words: alfalfa, in vitro, gamma rays, PEG

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Jurnal Ilmu Nutrisi dan Teknologi Pakan

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