{"title":"猪细小病毒DNA的分子克隆，以期在细菌中合成病毒抗原。","authors":"A D Zaberezhny, A L Konorova","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>An expression of porcine parvovirus 1.05 kb DNA fragment was obtained in Escherichia coli under the control both of Pr-lambda and lac-promoters. The product of expression under Pr-control was demonstrated to show PPV-specific antigenic properties. Its electrophoretic mobility corresponded to a molecular weight of approximately 45 KD.</p>","PeriodicalId":8263,"journal":{"name":"Archiv fur experimentelle Veterinarmedizin","volume":"44 6","pages":"891-6"},"PeriodicalIF":0.0000,"publicationDate":"1990-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Molecular cloning of porcine parvovirus DNA for the purpose of obtaining viral antigen synthesis in bacteria.\",\"authors\":\"A D Zaberezhny, A L Konorova\",\"doi\":\"\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>An expression of porcine parvovirus 1.05 kb DNA fragment was obtained in Escherichia coli under the control both of Pr-lambda and lac-promoters. The product of expression under Pr-control was demonstrated to show PPV-specific antigenic properties. Its electrophoretic mobility corresponded to a molecular weight of approximately 45 KD.</p>\",\"PeriodicalId\":8263,\"journal\":{\"name\":\"Archiv fur experimentelle Veterinarmedizin\",\"volume\":\"44 6\",\"pages\":\"891-6\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1990-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Archiv fur experimentelle Veterinarmedizin\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Archiv fur experimentelle Veterinarmedizin","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Molecular cloning of porcine parvovirus DNA for the purpose of obtaining viral antigen synthesis in bacteria.
An expression of porcine parvovirus 1.05 kb DNA fragment was obtained in Escherichia coli under the control both of Pr-lambda and lac-promoters. The product of expression under Pr-control was demonstrated to show PPV-specific antigenic properties. Its electrophoretic mobility corresponded to a molecular weight of approximately 45 KD.
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