自由生和共生缓生日本根瘤菌对琥珀酸利用的反应

L. Rybachenko, S. Kots, P. Pukhtaievych, O. R. Rybachenko, S. Omelchuk
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摘要

目标。研究不同浓度琥珀酸对缓生根瘤菌生长强度及其参与形成的共生系统活性的影响。方法。微生物学,生理学,统计学,气相色谱。结果。结果发现,无论浓度如何,T21-2菌株对琥珀酸的反应都比PC08菌株更明显。在根瘤菌培养基中添加浓度为0.01和0.02 g/L的琥珀酸可使T21- 2和PC08菌株的细胞滴度升高,而浓度为0.2 g/L的琥珀酸对所研究的菌株有毒性作用。在种子处理和接种悬浮液中添加浓度为0.01 g/L的琥珀酸,显著激活了T21-2根瘤菌形成的大豆共生系统的形成和功能过程。当浓度为0.02 g/L时,在所研究的菌株中,细菌细胞的生长速度最高,并且在培养的第3天和第4天,在不添加酸的情况下,细菌细胞的滴度相对于根瘤菌都有所增加。同时,无论采用何种施用方式,该浓度的琥珀酸均部分抑制了T21-2菌株的结瘤活性及其参与形成的共生系统的固氮活性。结论。基于已发现的琥珀酸对缓生根瘤菌纯培养生长和共生系统形成及功能的影响,建议在接种悬浮液中添加浓度为0.01 g/L的琥珀酸,并在播前处理中添加琥珀酸,以提高大豆-根瘤菌共生系统的活性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
REACTION OF FREE-LIVING AND SYMBIOTIC BRADYRHIZOBIUM JAPONICUM ON THE USE OF SUCCINIC ACID
Objective. Find out the influence of different concentrations of succinic acid on the intensity of growth of Bradyrhizobium japonicim and the activity of symbiotic systems created with their participation. Methods. Microbiological, physiological, statistical, gas chromatography. Results. It was found that the T21-2 strain had a more pronounced reaction to the use of succinic acid than the PC08 strain, regardless of its concentration. Addition of succinic acid to the culture medium of rhizobia at a concentration of 0.01 and 0.02 g/L led to an increase in the titre of bacterial cells of T21- 2 and PC08 strains, while at a concentration of 0.2 g/L it had a toxic effect on the studied strains. Succinic acid at a concentration of 0.01 g/L, both during seed treatment and as a component of the inoculation suspension, significantly activated the processes of formation and functioning of soybean symbiotic systems formed by the T21-2 rhizobia strain. At a concentration of 0.02 g/L, it provided the highest rate of growth of bacterial cells among the studied variants and led to an increase in their titre relative to rhizobia without adding acid, both on day three and four of cultivation. At the same time, regardless of the method of application, this concentration of succinic acid partially inhibited the nodulation activity of T21-2 strain and the nitrogen-fixing activity of the symbiotic systems formed with their participation. Conclusion. Based on the revealed effect of succinic acid on the growth of Bradyrhizobium japonicum in pure culture and on the formation and functioning of symbiosis, we recommend adding succinic acid at a concentration of 0.01 g/L, both as a component of the inoculation suspension, and for pre-sowing seed treatment to increase the activity of soybean-rhizobia symbiotic systems.
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