未灭活VTM与灭活VTM上SARS-COV-2 CT - RT-PCR值的差异

Dewi Inderiati, Retno Martini, Kiki Dinata, Citra Amaniah Anhar
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引用次数: 0

摘要

RT-PCR检测SARS-CoV-2的鼻咽拭子采集需要病毒转运介质(VTM),使用VTM非活化和灭活。本研究确定了非激活VTM和灭活VTM中SARS CoV-2 RT-PCR检测的CT值差异。本研究采用横断面法对RSU 20例确诊的SARS-CoV-2阳性样本进行对比分析。Suyoto博士采用SPSS进行T-Independent检验(N基因)和Mann-Whitney检验(ORF1ab基因),α=0.05。结果显示,未灭活VTM的N基因和ORF1ab基因的平均CT值分别为33.56±1.39和35.4±1.94,而灭活VTM的N和ORF1ab基因的平均CT值分别为32.24±1.44和33.93±3.22。采用t独立检验(N基因)和Mann Whitney检验(ORF1ab基因)对未灭活的VTM和灭活的VTM进行SARS - CoV-2 RT-PCR检测,p值为0.05,Ct基因N (p=0.006)和ORF1ab基因(p=0.028)的平均差异。这表明非激活VTM和灭活VTM的SARS CoV-2 RT-PCR检测的平均C - CT值存在差异。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Difference in CT RT-PCR Value of SARS-COV-2 on VTM Non-inactivated with VTM Inavtivated
Nasopharyngeal swab collection for SARS-CoV-2 examination by RT-PCR requires Viral Transport Medium (VTM) using VTM non-activated and inactivated. This study determined the difference in CT values for SARS CoV-2 RT-PCR examination in non-activated VTM and inactivated VTM. This study is a comparative analytical study with a cross-sectional method using 20 confirmed positive samples for SARS-CoV-2 at RSU. Dr. Suyoto was analyzed by the T-Independent test (N gene) and Mann-Whitney test (ORF1ab gene) using SPSS with α=0.05. The results showed that VTM non-inactivated had a mean CT value of the N gene 33.56 ± 1.39 and the ORF1ab gene 35.4 ± 1.94, while VTM inactivated had an average CT value of 32.24 ± 1.44 for the N and ORF1ab gene 33.93 ± 3.22. Statistical analysis was tested by the T-independent test (N gene), and the Mann Whitney test (ORF1ab gene) between the use of VTM non-inactivated and VTM inactivated in the SARS CoV-2 RT-PCR examination, obtained p-value 0.05 with a mean difference the results of Ct gene n (p=0.006) and gene ORF1ab (p=0.028). This indicated a difference in the mean C CT value of the SARS CoV-2 RT-PCR examination in non-activated VTM and inactivated VTM.
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