亚微流体装置量化细胞表面和电荷密度

2019 IEEE 2nd Ukraine Conference on Electrical and Computer Engineering (UKRCON) Pub Date : 2019-07-02 DOI:10.1109/UKRCON.2019.8879988

O. Borikov, O. M. Morozova, V. Berest

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引用次数: 1

摘要

本研究的目的是建立一个实验装置的原型，通过测量细胞的电泳迁移率和估计zeta电位来研究生物细胞表面的电学特性。改进了亚微流控室中细胞电泳的显微观察方法，采用实时视频采集技术。提出了对接收到的视频图像进行分析的基本方法，以便正确地确定细胞的线速度和电泳速度，从而进一步计算zeta电位。对获得的实验数据进行自动化处理，可以更快地对细胞群内参数分布进行统计分析。红细胞的测试测量和设备的操作指南已经开发出来。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Sub-Microfluidic Setup to Quantify Cell Surface & Charge Density

The purpose of this study is to create a prototype of an experimental setup for studying the electrical properties of biological cell surface by measuring electrophoretic mobility of cells and estimating zeta potential. We improved the microscopic method of observing cell electrophoresis in sub-microfluidic chamber with real-time video capture. The basic approaches to the analysis of received video images were figured out in order to correctly determine the linear and electrophoretic velocity of cells for further calculation of zeta potential. Automated processing of obtained experimental data allows faster statistical analysis of distribution of parameters within a cell population. Test measurements on erythrocytes and guidelines for the operation of the device have been developed.

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2019 IEEE 2nd Ukraine Conference on Electrical and Computer Engineering (UKRCON)

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