Development of Practical Media and Fermentative Technique for Mass Cultivation from Agricultural and Livestock Microorganism

Agricultural and livestock microorganisms including bacteria, yeast and fungi have been used as eco-friendly biological agent in plant growth promoting, disease suppression and odor reduction. In this study, we have optimized culture conditions of three species, such as Bacillus subtilis, Saccharomyces cerevisiae and Lactobacillus casei that could act as effective biological agent by producing growth-promoting factors to crops. They also reduce harmful odors generated from livestock manure. We made up the optimal media recipes for Bacillus subtilis, Saccharomyces cerevisae and Lactobacillus casei named as MMS (glucose 0.5%, soy bean flour 0.8%, NaCl 0.15%, K2HPO4 0.25%, Na2CO3 0.05%, MgSO4 0.1%), YSM (dextrose 1%, soytone 0.5%, yeast extract 0.5%) and LSM (glucose 2%, soytone 0.5%, casein milk 0.5%, NaAc 0.5%, KH2PO4 0.2%, MgSO4 0.002%, MnSO4 0.05%), respectively. The culture period of three microorganisms were determined on 30 hours in 500 L fermenter. In addition, the maximal cell growth of Bacillus subtilis, Saccharomyces cerevisae and Lactobacillus casei was measured as 1.4×10 cfu/mL, 6.4×10 cfu/mL, 3.3×10 cfu/mL, respectively. From above results, we represent that development of practical media and cultural techniques can economize heavily the cost for mass production of agricultural and livestock microorganisms. Additional key words: Bacillus subtilis, Saccharomyces cerevisiae, Lactobacillus casei, Practical media, Fermentative technique 서 론 농업현장에 있어 농약과 비료는 근대 과학기술의