农畜微生物规模化培养实用培养基及发酵技术的开发

Jun-Kyung Park, Sun-il Seo, G. Han, Kong-Min Kim, Dae-Hyuk Kim, Jaekyeong Song, P. Kim
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引用次数: 1

摘要

包括细菌、酵母菌和真菌在内的农畜微生物已被用作促进植物生长、抑制疾病和减少气味的生态友好型生物制剂。本研究优化了枯草芽孢杆菌、酿酒酵母菌和干酪乳杆菌3种可作为有效生物制剂对作物产生促生长因子的培养条件。它们还能减少牲畜粪便产生的有害气味。确定枯草芽孢杆菌、酿酒酵母和干酪乳杆菌的最佳培养基配方,分别为MMS(葡萄糖0.5%、豆粉0.8%、NaCl 0.15%、K2HPO4 0.25%、Na2CO3 0.05%、MgSO4 0.1%)、YSM(葡萄糖1%、豆粕0.5%、酵母膏0.5%)和LSM(葡萄糖2%、豆粕0.5%、酪蛋白乳0.5%、NaAc 0.5%、KH2PO4 0.2%、MgSO4 0.002%、MnSO4 0.05%)。测定了三种微生物在500 L发酵罐中30小时的培养周期。枯草芽孢杆菌、酿酒酵母菌和干酪乳杆菌的最大细胞生长分别为1.4×10 cfu/mL、6.4×10 cfu/mL、3.3×10 cfu/mL。从以上结果来看,我们表明实用的培养基和培养技术的发展可以大大节省农业和牲畜微生物大规模生产的成本。关键词:枯草芽孢杆菌,酿酒酵母菌,干酪乳杆菌,实用培养基,发酵技术
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Development of Practical Media and Fermentative Technique for Mass Cultivation from Agricultural and Livestock Microorganism
Agricultural and livestock microorganisms including bacteria, yeast and fungi have been used as eco-friendly biological agent in plant growth promoting, disease suppression and odor reduction. In this study, we have optimized culture conditions of three species, such as Bacillus subtilis, Saccharomyces cerevisiae and Lactobacillus casei that could act as effective biological agent by producing growth-promoting factors to crops. They also reduce harmful odors generated from livestock manure. We made up the optimal media recipes for Bacillus subtilis, Saccharomyces cerevisae and Lactobacillus casei named as MMS (glucose 0.5%, soy bean flour 0.8%, NaCl 0.15%, K2HPO4 0.25%, Na2CO3 0.05%, MgSO4 0.1%), YSM (dextrose 1%, soytone 0.5%, yeast extract 0.5%) and LSM (glucose 2%, soytone 0.5%, casein milk 0.5%, NaAc 0.5%, KH2PO4 0.2%, MgSO4 0.002%, MnSO4 0.05%), respectively. The culture period of three microorganisms were determined on 30 hours in 500 L fermenter. In addition, the maximal cell growth of Bacillus subtilis, Saccharomyces cerevisae and Lactobacillus casei was measured as 1.4×10 cfu/mL, 6.4×10 cfu/mL, 3.3×10 cfu/mL, respectively. From above results, we represent that development of practical media and cultural techniques can economize heavily the cost for mass production of agricultural and livestock microorganisms. Additional key words: Bacillus subtilis, Saccharomyces cerevisiae, Lactobacillus casei, Practical media, Fermentative technique 서 론 농업현장에 있어 농약과 비료는 근대 과학기술의
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