In-vitro evaluation of intermediate irrigants for removal of para-chloroaniline from the tooth during root canal treatment

Introduction: The need for root canal treatment is to eliminate bacteria from the infected canal system and to prevent recontamination. In this case, most common irrigant used in root canal treatment are sodium hypochlorite (NaOCl) and 2% chlorhexidinegluconate (CHX) have been recommended as a less toxic alternative. This treatment of using chemical irrigants for eliminating bacteria leads to a colour formation in the tooth by formation of parachloroaniline (PCA). Our study focuses on the spectrophotometric analysis of PCA formation during the NaOCl and CHX are mixed and thereby using intermediate irrigant solutions to prevention of orange brown precipitate. Spectrophotometer works on the principle of Beer Lambert’s Law in which - max determination of PCA was done using a wavelength scan between 350nm to 550nm. Furthermore, three different intermediate irrigant solutions were tested to analyse the efficient solution system for prevention of the orange brown precipitate. Group A fresh lime juice extract, group B 14.25% EDTA and group C 70% ethanol was used.Group A (lime group) exhibited the least OD value among all the experimental groups followed by group B (14.25% EDTA) and group C(70% Ethanol) and also statistically proved. Spectrophotometric analysis showed freshly prepared lime juice extract was found to be very effective in prevention of PCA precipitate followed by 14.25% EDTA and 70% ethanol. Thus, this study gives and insight about the analysis and novel ways of prevention of PCA during root canal treatment